| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab169186-25T (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 25T | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $151.90 | |
| Ab169186-100T | 100T | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $284.90 |
Recombinant; CD20 antibody (PE); Flow
| Product Name | Recombinant CD20 Antibody (PE) - Primary antibody, for Flow various applications, from Human, generated in Human, specific to Human CD20 / MS4A1, Human IgG1 |
|---|---|
| Synonyms | B-lymphocyte surface antigen B1 | Bp35 | Leukocyte surface antigen Leu-16 | Membrane-spanning 4-domains subfamily A member 1 |
| Specifications & Purity | ExactAb™, Validated, Azide Free, Recombinant, 0.05mg/mL; 10uL/Test |
| Host Species | Human |
| Host species | Human |
| Specificity | Human CD20 / MS4A1 |
| Application | Flow |
| Positive Control | Flow: Raji |
| Conjugation | PE |
| Grade | Azide Free, ExactAb™, Recombinant, Validated |
| Product Description | Background: |
| Antibody Type | Primary antibody |
|---|---|
| Isotype | Human IgG1 |
| Form | Liquid |
| Concentration | 0.05mg/mL; 10uL/Test |
| Storage Temp | Store at 2-8°C,Protected from light,Freeze prohibited,Avoid repeated freezing and thawing |
| Shipped In | Wet ice |
| Stability And Storage | Shipped at 4°C. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
Recombinant CD20 Antibody (PE) (Ab169186)-Flow
Raji Cells are stained with Recombinant CD20 Antibody (PE) (Ab169186) (Red). Unstained Raji Cells (Gray) are used as control.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry | 10µL/Test For flow cytometric staining, the suggested use of this reagent is 10 µL per million cells in 100 µL staining volume or 10 µL per 100 µL of whole blood. |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0700601 | Certificate of Analysis | Jul 14, 2023 | Ab169186 |
| ZJ23F0700600 | Certificate of Analysis | Jul 14, 2023 | Ab169186 |
| 1. Kuijpers TW, Bende RJ, Baars PA, Grummels A, Derks IA, Dolman KM, Beaumont T, Tedder TF, van Noesel CJ, Eldering E et al.. (2010) CD20 deficiency in humans results in impaired T cell-independent antibody responses.. J Clin Invest, 120 (1): (214-22). [PMID:20038800] [10.1021/op500134e] |
| 2. Stein R, Qu Z, Chen S, Rosario A, Shi V, Hayes M, Horak ID, Hansen HJ, Goldenberg DM. (2004) Characterization of a new humanized anti-CD20 monoclonal antibody, IMMU-106, and Its use in combination with the humanized anti-CD22 antibody, epratuzumab, for the therapy of non-Hodgkin's lymphoma.. Clin Cancer Res, 10 (8): (2868-78). [PMID:15102696] [10.1021/op500134e] |
| 3. Le Garff-Tavernier M, Decocq J, de Romeuf C, Parizot C, Dutertre CA, Chapiro E, Davi F, Debré P, Prost JF, Teillaud JL et al.. (2011) Analysis of CD16+CD56dim NK cells from CLL patients: evidence supporting a therapeutic strategy with optimized anti-CD20 monoclonal antibodies.. Leukemia, 25 (1): (101-9). [PMID:20975664] [10.1021/op500134e] |
| 4. Li B, Xu L, Pi C, Yin Y, Xie K, Tao F, Li R, Gu H, Fang J. (2017) CD89-mediated recruitment of macrophages via a bispecific antibody enhances anti-tumor efficacy.. Oncoimmunology, 7 (1): (e1380142). [PMID:29296544] [10.1021/op500134e] |
| 5. Sun LL, Ellerman D, Mathieu M, Hristopoulos M, Chen X, Li Y, Yan X, Clark R, Reyes A, Stefanich E et al.. (2015) Anti-CD20/CD3 T cell-dependent bispecific antibody for the treatment of B cell malignancies.. Sci Transl Med, 7 (287): (287ra70). [PMID:25972002] [10.1021/op500134e] |
| 6. Tedder, T F TF, Streuli, M M, Schlossman, S F SF and Saito, H H.. (1988) Isolation and structure of a cDNA encoding the B1 (CD20) cell-surface antigen of human B lymphocytes.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2448768] |
| 7. Einfeld, D A DA, Brown, J P JP, Valentine, M A MA, Clark, E A EA and Ledbetter, J A JA.. (1988) Molecular cloning of the human B cell CD20 receptor predicts a hydrophobic protein with multiple transmembrane domains.. The EMBO journal, [PMID:2456210] |
| 8. Tedder, T F TF, Klejman, G G, Schlossman, S F SF and Saito, H H.. (1989) Structure of the gene encoding the human B lymphocyte differentiation antigen CD20 (B1).. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:2466899] |
| 9. Stamenkovic, I I and Seed, B B.. (1988) Analysis of two cDNA clones encoding the B lymphocyte antigen CD20 (B1, Bp35), a type III integral membrane protein.. The Journal of experimental medicine, (1): [PMID:3260267] |
| 10. Tedder, T F TF, Boyd, A W AW, Freedman, A S AS, Nadler, L M LM and Schlossman, S F SF.. (1985) The B cell surface molecule B1 is functionally linked with B cell activation and differentiation.. Journal of immunology (Baltimore, Md. : 1950), [PMID:3925015] |
| 11. Bubien, J K JK, Zhou, L J LJ, Bell, P D PD, Frizzell, R A RA and Tedder, T F TF.. (1993) Transfection of the CD20 cell surface molecule into ectopic cell types generates a Ca2+ conductance found constitutively in B lymphocytes.. The Journal of cell biology, [PMID:7684739] |
| 12. and Goldenberg, D M DM.. () The role of radiolabeled antibodies in the treatment of non-Hodgkin's lymphoma: the coming of age of radioimmunotherapy.. Critical reviews in oncology/hematology, [PMID:11418316] |
| 13. and Dillman, R O RO.. () Monoclonal antibody therapy for lymphoma: an update.. Cancer practice, [PMID:11879282] |
| 14. . (2003) Iodine-131 Tositumomab: (131)I-anti-B1 antibody, (131)I-tositumomab, anti-CD20 murine monoclonal antibody-I-131, B1, Bexxar, (131)I-anti-B1 antibody, iodine-131 tositumomab, iodine-131 anti-B1 antibody, tositumomab.. BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy, [PMID:12899647] |
| 15. Li, Haidong H, Ayer, Linda M LM, Lytton, Jonathan J and Deans, Julie P JP.. (2003) Store-operated cation entry mediated by CD20 in membrane rafts.. The Journal of biological chemistry, (24): [PMID:12920111] |
| 16. Du, Jiamu J and 8 more authors.. (2008) Crystal structure of chimeric antibody C2H7 Fab in complex with a CD20 peptide.. Molecular immunology, [PMID:18346788] |
| 17. Dörner, Thomas T and Burmester, Gerd R GR.. (2008) New approaches of B-cell-directed therapy: beyond rituximab.. Current opinion in rheumatology, [PMID:18388516] |
| 18. Polyak, Maria J MJ, Li, Haidong H, Shariat, Neda N and Deans, Julie P JP.. (2008) CD20 homo-oligomers physically associate with the B cell antigen receptor. Dissociation upon receptor engagement and recruitment of phosphoproteins and calmodulin-binding proteins.. The Journal of biological chemistry, (4): [PMID:18474602] |
| 19. Niederfellner, Gerhard G and 16 more authors.. (2011) Epitope characterization and crystal structure of GA101 provide insights into the molecular basis for type I/II distinction of CD20 antibodies.. Blood, (14): [PMID:21444918] |
| 20. Ivaldi, Corinne C and 6 more authors.. (2012) Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23.. PloS one, [PMID:22615937] |
| 21. Phuah, Jiayao J and 10 more authors.. (2016) Effects of B Cell Depletion on Early Mycobacterium tuberculosis Infection in Cynomolgus Macaques.. Infection and immunity, [PMID:26883591] |
| 22. Hayashi, Kazumi and 6 more authors.. (2016) Gemcitabine enhances rituximab-mediated complement-dependent cytotoxicity to B cell lymphoma by CD20 upregulation.. Cancer science, [PMID:26920337] |
