A synthetic peptide derived from human CD27 (AA 244-258).
Positive Control
WB: Raji, Ramos lysates
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human CD27 Antibody, Recombinant, could be used for WB and so on.
Application: WB: 1/500-1/1000
Protein Function Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
1.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant CD27 Antibody (Ab094349) - Western Blot All lanes: Recombinant CD27 Antibody (Ab094349) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa Observed band size: 50 kDa Exposure time: 8.4 s
1.Loenen, W A WA and 5 more authors.. (1992) Genomic organization and chromosomal localization of the human CD27 gene.. Journal of immunology (Baltimore, Md. : 1950), (15):[PMID:1334106]
2.Camerini, D D, Walz, G G, Loenen, W A WA, Borst, J J and Seed, B B.. (1991) The T cell activation antigen CD27 is a member of the nerve growth factor/tumor necrosis factor receptor gene family.. Journal of immunology (Baltimore, Md. : 1950), (1):[PMID:1655907]
3.Prasad, K V KV and 6 more authors.. (1997) CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein.. Proceedings of the National Academy of Sciences of the United States of America, (10):[PMID:9177220]
4.Gravestein, L A LA and 5 more authors.. (1998) The TNF receptor family member CD27 signals to Jun N-terminal kinase via Traf-2.. European journal of immunology, [PMID:9692890]
5.Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161]
6.Scherer, Steven E SE and 216 more authors.. (2006) The finished DNA sequence of human chromosome 12.. Nature, (16):[PMID:16541075]
7.van Montfrans, Joris M JM and 13 more authors.. (2012) CD27 deficiency is associated with combined immunodeficiency and persistent symptomatic EBV viremia.. The Journal of allergy and clinical immunology, [PMID:22197273]
8.Salzer, Elisabeth E and 14 more authors.. (2013) Combined immunodeficiency with life-threatening EBV-associated lymphoproliferative disorder in patients lacking functional CD27.. Haematologica, [PMID:22801960]
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