| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab178034-10T (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10T | In stock | $19.90 | |
| Ab178034-25T | 25T | In stock | $29.90 | |
| Ab178034-100T | 100T | In stock | $99.90 |
Recombinant; CD3 Antibody (APC); Flow
| Product Name | Recombinant CD3 Antibody (APC) - Primary antibody, specific to CD3, Mouse IgG2a |
|---|---|
| Synonyms | CD_antigen: CD3e | CD3 antigen, delta subunit | CD3 | CD3d antigen | CD3d antigen, delta polypeptide (TiT3 complex) | CD3d molecule, delta (CD3-TCR complex) | CD3-DELTA | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, eps |
| Specifications & Purity | ExactAb™, Validated, Azide Free, Recombinant, 5μL/ test |
| Host species | Mouse |
| Specificity | CD3 |
| Positive Control | Flow: Jurkat |
| Conjugation | APC |
| Grade | Azide Free, ExactAb™, Recombinant, Validated |
| Product Description | Application: |
| Isotype | Mouse IgG2a |
|---|---|
| Light Chain Type | kappa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 5μL/ test |
| Storage Temp | Store at 2-8°C,Protected from light,Freeze prohibited |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C for one year. Do not freeze. Protect from light. |
Recombinant CD3 Antibody (APC) (Ab178034) - Flow Cytometry
Flow cytometry analysis of CD3 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD3 Antibody (APC) (Ab178034) at 5μL/test for 1 hour at 4°C. Blue - Isotype control. Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry |
5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. "> 5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0102314 | Certificate of Analysis | Jan 08, 2024 | Ab178034 |
| ZJ24F0102303 | Certificate of Analysis | Jan 08, 2024 | Ab178034 |
| ZJ24F0102302 | Certificate of Analysis | Jan 08, 2024 | Ab178034 |
| ZJ23R1200201 | Certificate of Analysis | Dec 21, 2023 | Ab178034 |
| 1. Linke R, Klein A, Seimetz D. (2010) Catumaxomab: clinical development and future directions.. MAbs, 2 (2): (129-36). [PMID:20190561] [10.1021/op500134e] |
| 2. Soudais C, de Villartay JP, Le Deist F, Fischer A, Lisowska-Grospierre B. (1993) Independent mutations of the human CD3-epsilon gene resulting in a T cell receptor/CD3 complex immunodeficiency.. Nat Genet, 3 (1): (77-81). [PMID:8490660] [10.1021/op500134e] |
| 3. de Saint Basile G, Geissmann F, Flori E, Uring-Lambert B, Soudais C, Cavazzana-Calvo M, Durandy A, Jabado N, Fischer A, Le Deist F. (2004) Severe combined immunodeficiency caused by deficiency in either the delta or the epsilon subunit of CD3.. J Clin Invest, 114 (10): (1512-7). [PMID:15546002] [10.1021/op500134e] |
| 4. Borroto A, Reyes-Garau D, Jiménez MA, Carrasco E, Moreno B, Martínez-Pasamar S, Cortés JR, Perona A, Abia D, Blanco S et al.. (2016) First-in-class inhibitor of the T cell receptor for the treatment of autoimmune diseases.. Sci Transl Med, 8 (370): (370ra184). [PMID:28003549] [10.1021/op500134e] |
| 5. Sun LL, Ellerman D, Mathieu M, Hristopoulos M, Chen X, Li Y, Yan X, Clark R, Reyes A, Stefanich E et al.. (2015) Anti-CD20/CD3 T cell-dependent bispecific antibody for the treatment of B cell malignancies.. Sci Transl Med, 7 (287): (287ra70). [PMID:25972002] [10.1021/op500134e] |
| 6. Lanier, L L LL, Chang, C C, Spits, H H and Phillips, J H JH.. (1992) Expression of cytoplasmic CD3 epsilon proteins in activated human adult natural killer (NK) cells and CD3 gamma, delta, epsilon complexes in fetal NK cells. Implications for the relationship of NK and T lymphocytes.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:1387664] |
| 7. Barber, E K EK, Dasgupta, J D JD, Schlossman, S F SF, Trevillyan, J M JM and Rudd, C E CE.. (1989) The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2470098] |
| 8. Gold, D P DP and 6 more authors.. (1986) Isolation of cDNA clones encoding the 20K non-glycosylated polypeptide chain of the human T-cell receptor/T3 complex.. Nature, [PMID:3012357] |
| 9. Salomon, Arthur R AR and 10 more authors.. (2003) Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry.. Proceedings of the National Academy of Sciences of the United States of America, (21): [PMID:12522270] |
| 10. Brill, Laurence M LM and 5 more authors.. (2004) Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.. Analytical chemistry, (15): [PMID:15144186] |
| 11. Gimferrer, Idoia I and 8 more authors.. (2004) Relevance of CD6-mediated interactions in T cell activation and proliferation.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:15294938] |
| 12. Arnett, Kelly L KL, Harrison, Stephen C SC and Wiley, Don C DC.. (2004) Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment.. Proceedings of the National Academy of Sciences of the United States of America, (16): [PMID:15534202] |
| 13. Gaglia, Jason and Kissler, Stephan.. (2019) Anti-CD3 Antibody for the Prevention of Type 1 Diabetes: A Story of Perseverance.. Biochemistry, (8): [PMID:31523950] |
| 14. Clevers, H C HC, Dunlap, S S, Wileman, T E TE and Terhorst, C C.. (1988) Human CD3-epsilon gene contains three miniexons and is transcribed from a non-TATA promoter.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3267235] |
| 15. Fütterer, K K, Wong, J J, Grucza, R A RA, Chan, A C AC and Waksman, G G.. (1998) Structural basis for Syk tyrosine kinase ubiquity in signal transduction pathways revealed by the crystal structure of its regulatory SH2 domains bound to a dually phosphorylated ITAM peptide.. Journal of molecular biology, (21): [PMID:9698567] |
| 16. Mathur D, Root AR, Bugaj-Gaweda B, Bisulco S, Tan X, Fang W, Kearney JC, Lucas J, Guffroy M, Golas J et al.. (2020) A Novel GUCY2C-CD3 T-Cell Engaging Bispecific Construct (PF-07062119) for the Treatment of Gastrointestinal Cancers.. Clin Cancer Res, 26 (9): (2188-2202). [PMID:31996389] [10.1021/op500134e] |
