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Application: Flow: 5μL/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Product Properties
Isotype
Human IgG1
Light Chain Type
kappa
Purification Method
Protein A purified
Form
Liquid
Concentration
5μL/test
Storage Temp
Store at 2-8°C,Protected from light,Freeze prohibited
Shipped In
Wet ice
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C for one year. Do not freeze. Protect from light.
Images
Recombinant CD30 Antibody (APC) (Ab176339) - Flow Cytometry Flow cytometry analysis of CD30 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD30 Antibody (APC) (Ab176339) at 5μL/test for 1 hour at 4°C. Blue - Isotype control. Black - Unlabelled control, cells without incubation with primary antibody.
Associated Targets(Human)
TNFRSF8TclinTumor necrosis factor receptor superfamily member 8 (0 Activities)
1.Reusch U, Burkhardt C, Fucek I, Le Gall F, Le Gall M, Hoffmann K, Knackmuss SH, Kiprijanov S, Little M, Zhukovsky EA. (2014) A novel tetravalent bispecific TandAb (CD30/CD16A) efficiently recruits NK cells for the lysis of CD30+ tumor cells.. MAbs, 6 (3):(728-39). [PMID:24670809]
2.Dürkop, H H and 5 more authors.. (1992) Molecular cloning and expression of a new member of the nerve growth factor receptor family that is characteristic for Hodgkin's disease.. Cell, (7):[PMID:1310894]
3.Jung, W W and 6 more authors.. (1994) Opposite effects of the CD30 ligand are not due to CD30 mutations: results from cDNA cloning and sequence comparison of the CD30 antigen from different sources.. Molecular immunology, [PMID:7527901]
4.Smith, C A CA and 9 more authors.. (1993) CD30 antigen, a marker for Hodgkin's lymphoma, is a receptor whose ligand defines an emerging family of cytokines with homology to TNF.. Cell, (2):[PMID:8391931]
5.Lee, S Y SY, Park, C G CG and Choi, Y Y.. (1996) T cell receptor-dependent cell death of T cell hybridomas mediated by the CD30 cytoplasmic domain in association with tumor necrosis factor receptor-associated factors.. The Journal of experimental medicine, (1):[PMID:8627180]
6.Horie, R R and 9 more authors.. (1996) A variant CD30 protein lacking extracellular and transmembrane domains is induced in HL-60 by tetradecanoylphorbol acetate and is expressed in alveolar macrophages.. Blood, (1):[PMID:8839832]
7.Aizawa, S S and 9 more authors.. (1997) Tumor necrosis factor receptor-associated factor (TRAF) 5 and TRAF2 are involved in CD30-mediated NFkappaB activation.. The Journal of biological chemistry, (24):[PMID:8999898]
8.Boucher, L M LM, Marengère, L E LE, Lu, Y Y, Thukral, S S and Mak, T W TW.. (1997) Binding sites of cytoplasmic effectors TRAF1, 2, and 3 on CD30 and other members of the TNF receptor superfamily.. Biochemical and biophysical research communications, (28):[PMID:9168896]
9.Mizushima, S S and 8 more authors.. (1998) Cloning and characterization of a cDNA encoding the human homolog of tumor necrosis factor receptor-associated factor 5 (TRAF5).. Gene, (30):[PMID:9511754]
10.Francisco, Joseph A JA and 14 more authors.. (2003) cAC10-vcMMAE, an anti-CD30-monomethyl auristatin E conjugate with potent and selective antitumor activity.. Blood, (15):[PMID:12714494]
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