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Application: Flow: 5μL/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Product Properties
Isotype
Human IgG1
Light Chain Type
kappa
Purification Method
Protein A purified
Form
Liquid
Concentration
5μL/test
Storage Temp
Store at 2-8°C,Protected from light,Freeze prohibited
Shipped In
Wet ice
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C for one year. Do not freeze. Protect from light.
Images
Recombinant CD30 Antibody (PE) (Ab176338) - Flow Cytometry Flow cytometry analysis of CD30 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD30 Antibody (PE) (Ab176338) at 5μL/test for 1 hour at 4°C. Blue - Isotype control. Black - Unlabelled control, cells without incubation with primary antibody.
Associated Targets(Human)
TNFRSF8TclinTumor necrosis factor receptor superfamily member 8 (0 Activities)
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. ">
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Certificates
Certificate of Analysis(COA)
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
1.Reusch U, Burkhardt C, Fucek I, Le Gall F, Le Gall M, Hoffmann K, Knackmuss SH, Kiprijanov S, Little M, Zhukovsky EA. (2014) A novel tetravalent bispecific TandAb (CD30/CD16A) efficiently recruits NK cells for the lysis of CD30+ tumor cells.. MAbs, 6 (3):(728-39). [PMID:24670809][10.1021/op500134e]
2.Dürkop, H H and 5 more authors.. (1992) Molecular cloning and expression of a new member of the nerve growth factor receptor family that is characteristic for Hodgkin's disease.. Cell, (7):[PMID:1310894]
3.Jung, W W and 6 more authors.. (1994) Opposite effects of the CD30 ligand are not due to CD30 mutations: results from cDNA cloning and sequence comparison of the CD30 antigen from different sources.. Molecular immunology, [PMID:7527901]
4.Smith, C A CA and 9 more authors.. (1993) CD30 antigen, a marker for Hodgkin's lymphoma, is a receptor whose ligand defines an emerging family of cytokines with homology to TNF.. Cell, (2):[PMID:8391931]
5.Lee, S Y SY, Park, C G CG and Choi, Y Y.. (1996) T cell receptor-dependent cell death of T cell hybridomas mediated by the CD30 cytoplasmic domain in association with tumor necrosis factor receptor-associated factors.. The Journal of experimental medicine, (1):[PMID:8627180]
6.Horie, R R and 9 more authors.. (1996) A variant CD30 protein lacking extracellular and transmembrane domains is induced in HL-60 by tetradecanoylphorbol acetate and is expressed in alveolar macrophages.. Blood, (1):[PMID:8839832]
7.Aizawa, S S and 9 more authors.. (1997) Tumor necrosis factor receptor-associated factor (TRAF) 5 and TRAF2 are involved in CD30-mediated NFkappaB activation.. The Journal of biological chemistry, (24):[PMID:8999898]
8.Boucher, L M LM, Marengère, L E LE, Lu, Y Y, Thukral, S S and Mak, T W TW.. (1997) Binding sites of cytoplasmic effectors TRAF1, 2, and 3 on CD30 and other members of the TNF receptor superfamily.. Biochemical and biophysical research communications, (28):[PMID:9168896]
9.Mizushima, S S and 8 more authors.. (1998) Cloning and characterization of a cDNA encoding the human homolog of tumor necrosis factor receptor-associated factor 5 (TRAF5).. Gene, (30):[PMID:9511754]
10.Francisco, Joseph A JA and 14 more authors.. (2003) cAC10-vcMMAE, an anti-CD30-monomethyl auristatin E conjugate with potent and selective antitumor activity.. Blood, (15):[PMID:12714494]
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