Recombinant Cdc34 Antibody - Primary antibody, specific to Cdc34, Rabbit IgG

    Application:
  • IF/ICC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab095618
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab095618-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$79.90
Ab095618-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$239.90
Ab095618-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$379.90
Ab095618-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,099.90

Recombinant; Rabbit anti Human Cdc34 Antibody; WB, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant Cdc34 Antibody - Primary antibody, specific to Cdc34, Rabbit IgG
SynonymsCdc 34 antibody | Cdc34 antibody | Cell division cycle 34 antibody | Cell division cycle 34 homolog (S. cerevisiae) antibody | Cell division cycle 34 homolog antibody | E2 CDC34antibody | UB2R1_HUMAN antibody | UBC 3 antibody | UBC3 antibody | UBCH3 antib
Specifications & PurityExactAb™, Validated, Recombinant, Lot by Lot
Host speciesRabbit
SpecificityCdc34
ImmunogenA synthetic peptide derived from human Cdc34 (AA 2-236).
Positive ControlWB: Jurkat, Hep G2, K562, A549, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Cdc34 Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/2000
ICC/IF: 1/50-1/200
Protein Function:
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase throught its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Cdc34 Antibody (Ab095618) - Western Blot
All lanes: Recombinant Cdc34 Antibody (Ab095618) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 27 kDa
Observed band size: 31 kDa
Exposure time: 42.7 s

Recombinant Cdc34 Antibody (Ab095618) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Cdc34 Antibody (Ab095618).

Recombinant Cdc34 Antibody (Ab095618) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Cdc34 Antibody (Ab095618) at 1/25 dilution.

Recombinant Cdc34 Antibody (Ab095618) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Cdc34 Antibody (Ab095618) at 1/100 dilution.

Associated Targets(Human)

CDC34 Tbio Ubiquitin-conjugating enzyme E2 R1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/2000

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1/500-1/2000

IF/ICC 1/50-1/200

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1/50-1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0607513Certificate of AnalysisJun 27, 2024 Ab095618
ZJ24F0607512Certificate of AnalysisJun 27, 2024 Ab095618

Related Documents

Solution Calculators