Recombinant CDCA5 Antibody - Primary antibody, specific to CDCA5, Rabbit IgG
Synonyms
Cell division cycle-associated protein 5 | p35
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.85 mg/mL
Host species
Rabbit
Specificity
CDCA5
Immunogen
A synthetic peptide derived from human CDCA5 (AA 200-252)
Positive Control
WB: Jurkat, K562, Hep G2 and HEK293 cell lysates. IHC: Human colon tissues. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human CDCA5 Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPAL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.85 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
1.Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)):(4493-4501). [PMID:19413330]
2. and Walker, M G MG.. (2001) Drug target discovery by gene expression analysis: cell cycle genes.. Current cancer drug targets, [PMID:12188893]
3.Rankin, Susannah S, Ayad, Nagi G NG and Kirschner, Marc W MW.. (2005) Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates.. Molecular cell, (15):[PMID:15837422]
4.Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243]
5.Schmitz, Julia J, Watrin, Erwan E, Lénárt, Péter P, Mechtler, Karl K and Peters, Jan-Michael JM.. (2007) Sororin is required for stable binding of cohesin to chromatin and for sister chromatid cohesion in interphase.. Current biology : CB, (3):[PMID:17349791]
6.Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5):[PMID:18669648]
7.Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18):[PMID:19690332]
8.Nishiyama, Tomoko T and 10 more authors.. (2010) Sororin mediates sister chromatid cohesion by antagonizing Wapl.. Cell, (24):[PMID:21111234]
9.Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15):[PMID:21406692]
10.Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4):[PMID:23186163]
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