| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab096038-10μl | 10μl | In stock | $66.90 | |
| Ab096038-50μl | 50μl | In stock | $199.90 | |
| Ab096038-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab096038-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,499.90 |
Recombinant; Rabbit anti Human CENPA Antibody; WB; Unconjugated
| Product Name | Recombinant CENPA Antibody - Primary antibody, specific to CENPA, Rabbit IgG |
|---|---|
| Synonyms | CENP A antibody | CENP-A antibody | cenpa antibody | CENPA_HUMAN antibody | Centromere autoantigen A antibody | Centromere protein A 17kDa antibody | Centromere protein A antibody | Histone H3 like centromeric protein A antibody | Histone H3-like centrome |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.6 mg/mL |
| Host species | Rabbit |
| Specificity | CENPA |
| Immunogen | A synthetic peptide derived from human CENPA (AA 122-140) |
| Positive Control | WB: K562, HeLa, A375 and HT-29 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human CENPA Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant CENPA Antibody (Ab096038) - Western Blot
All lanes: Recombinant CENPA Antibody (Ab096038) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 15, 17 kDa
Exposure time: 30.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303166 | Certificate of Analysis | Mar 13, 2024 | Ab096038 |
| ZJ24F0303165 | Certificate of Analysis | Mar 13, 2024 | Ab096038 |
| 1. Sullivan, K F KF, Hechenberger, M M and Masri, K K.. (1994) Human CENP-A contains a histone H3 related histone fold domain that is required for targeting to the centromere.. The Journal of cell biology, [PMID:7962047] |
| 2. Shelby, R D RD, Vafa, O O and Sullivan, K F KF.. (1997) Assembly of CENP-A into centromeric chromatin requires a cooperative array of nucleosomal DNA contact sites.. The Journal of cell biology, (10): [PMID:9024683] |
| 3. Muro, Y Y and 6 more authors.. (2000) Autoepitopes on autoantigen centromere protein-A (CENP-A) are restricted to the N-terminal region, which has no homology with histone H3.. Clinical and experimental immunology, [PMID:10759786] |
| 4. Lomonte, P P, Sullivan, K F KF and Everett, R D RD.. (2001) Degradation of nucleosome-associated centromeric histone H3-like protein CENP-A induced by herpes simplex virus type 1 protein ICP0.. The Journal of biological chemistry, (23): [PMID:11053442] |
| 5. Zeitlin, S G SG, Shelby, R D RD and Sullivan, K F KF.. (2001) CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis.. The Journal of cell biology, (24): [PMID:11756469] |
| 6. Kunitoku, Naoko N and 9 more authors.. (2003) CENP-A phosphorylation by Aurora-A in prophase is required for enrichment of Aurora-B at inner centromeres and for kinetochore function.. Developmental cell, [PMID:14667408] |
| 7. Black, Ben E BE and 5 more authors.. (2004) Structural determinants for generating centromeric chromatin.. Nature, (29): [PMID:15282608] |
| 8. Sullivan, Beth A BA and Karpen, Gary H GH.. (2004) Centromeric chromatin exhibits a histone modification pattern that is distinct from both euchromatin and heterochromatin.. Nature structural & molecular biology, [PMID:15475964] |
| 9. Irvine, Danielle V DV and 6 more authors.. (2004) Chromosome size and origin as determinants of the level of CENP-A incorporation into human centromeres.. Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, [PMID:15702419] |
| 10. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 11. Foltz, Daniel R DR and 5 more authors.. (2006) The human CENP-A centromeric nucleosome-associated complex.. Nature cell biology, [PMID:16622419] |
| 12. Alonso, Alicia A and 8 more authors.. (2007) Co-localization of CENP-C and CENP-H to discontinuous domains of CENP-A chromatin at human neocentromeres.. Genome biology, [PMID:17651496] |
| 13. Orthaus, Sandra S and 6 more authors.. (2008) Assembly of the inner kinetochore proteins CENP-A and CENP-B in living human cells.. Chembiochem : a European journal of chemical biology, (4): [PMID:18072184] |
| 14. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 15. Foltz, Daniel R DR and 7 more authors.. (2009) Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP.. Cell, (1): [PMID:19410544] |
| 16. Dunleavy, Elaine M EM and 8 more authors.. (2009) HJURP is a cell-cycle-dependent maintenance and deposition factor of CENP-A at centromeres.. Cell, (1): [PMID:19410545] |
| 17. Hellwig, Daniela D and 5 more authors.. (2008) Live-cell imaging reveals sustained centromere binding of CENP-T via CENP-A and CENP-B.. Journal of biophotonics, [PMID:19412974] |
| 18. Trazzi, Stefania S and 6 more authors.. (2009) The C-terminal domain of CENP-C displays multiple and critical functions for mammalian centromere formation.. PloS one, (8): [PMID:19503796] |
| 19. Kim, Hyejin H and 7 more authors.. (2009) Cancer-upregulated gene 2 (CUG2), a new component of centromere complex, is required for kinetochore function.. Molecules and cells, (30): [PMID:19533040] |
| 20. Carroll, Christopher W CW, Silva, Mariana C C MC, Godek, Kristina M KM, Jansen, Lars E T LE and Straight, Aaron F AF.. (2009) Centromere assembly requires the direct recognition of CENP-A nucleosomes by CENP-N.. Nature cell biology, [PMID:19543270] |
| 21. Sekulic, Nikolina N, Bassett, Emily A EA, Rogers, Danielle J DJ and Black, Ben E BE.. (2010) The structure of (CENP-A-H4)(2) reveals physical features that mark centromeres.. Nature, (16): [PMID:20739937] |
| 22. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 23. Hu, Hao H and 11 more authors.. (2011) Structure of a CENP-A-histone H4 heterodimer in complex with chaperone HJURP.. Genes & development, (1): [PMID:21478274] |
| 24. Tachiwana, Hiroaki H and 11 more authors.. (2011) Crystal structure of the human centromeric nucleosome containing CENP-A.. Nature, (10): [PMID:21743476] |
| 25. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 26. Bailey, Aaron O AO and 11 more authors.. (2013) Posttranslational modification of CENP-A influences the conformation of centromeric chromatin.. Proceedings of the National Academy of Sciences of the United States of America, (16): [PMID:23818633] |
| 27. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 28. Dornblut, Carsten C and 11 more authors.. (2014) A CENP-S/X complex assembles at the centromere in S and G2 phases of the human cell cycle.. Open biology, (12): [PMID:24522885] |
| 29. Arimura, Yasuhiro Y and 8 more authors.. (2014) Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3.. Scientific reports, (19): [PMID:25408271] |
| 30. Wu, Ruoxi R, Yue, Yuan Y, Zheng, Xiangdong X and Li, Haitao H.. (2015) Molecular basis for histone N-terminal methylation by NRMT1.. Genes & development, (15): [PMID:26543159] |
| 31. Falk, Samantha J SJ and 5 more authors.. (2016) CENP-C directs a structural transition of CENP-A nucleosomes mainly through sliding of DNA gyres.. Nature structural & molecular biology, [PMID:26878239] |
| 32. Roulland, Yohan Y and 19 more authors.. (2016) The Flexible Ends of CENP-A Nucleosome Are Required for Mitotic Fidelity.. Molecular cell, (18): [PMID:27499292] |
