Recombinant; Rabbit anti Human CGBP Antibody; WB, IHC, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab096198-10μl | 10μl | In stock | $69.90 | |
| Ab096198-50μl | 50μl | In stock | $199.90 | |
| Ab096198-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab096198-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human CGBP Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Recombinant CGBP Antibody - Primary antibody, specific to CXXC1, Rabbit IgG |
|---|---|
| Synonyms | 2410002I16Rik | 5830420C16Rik | AI426635 | CFP 1 | CFP1 | CpG binding protein | CpG-binding protein | CXXC 1 | CXXC finger 1 | CXXC finger 1 PHD domain | CXXC finger protein 1 | CXXC type zinc finger protein 1 | CXXC-type zinc finger protein 1 | CXXC1 | C |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.7 mg/mL |
| Host species | Rabbit |
| Specificity | CXXC1 |
| Immunogen | A synthetic peptide derived from human CGBP (AA 331-350) |
| Positive Control | WB: HeLa, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human CGBP Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.7 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant CGBP Antibody (Ab096198) - Western Blot
All lanes: Recombinant CGBP Antibody (Ab096198) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 82 kDa
Exposure time: 5.0 s
Recombinant CGBP Antibody (Ab096198) - IF/ICC
Immunocytochemistry analysis of CGBP (green) in HeLa using Recombinant CGBP Antibody (Ab096198), and cells were counterstained with DAPI (blue).
Recombinant CGBP Antibody (Ab096198) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant CGBP Antibody (Ab096198). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303168 | Certificate of Analysis | Mar 13, 2024 | Ab096198 |
| ZJ24F0303167 | Certificate of Analysis | Mar 13, 2024 | Ab096198 |
| 1. Voo, K S KS, Carlone, D L DL, Jacobsen, B M BM, Flodin, A A and Skalnik, D G DG.. (2000) Cloning of a mammalian transcriptional activator that binds unmethylated CpG motifs and shares a CXXC domain with DNA methyltransferase, human trithorax, and methyl-CpG binding domain protein 1.. Molecular and cellular biology, [PMID:10688657] |
| 2. Fujino, T T and 5 more authors.. (2000) PCCX1, a novel DNA-binding protein with PHD finger and CXXC domain, is regulated by proteolysis.. Biochemical and biophysical research communications, (10): [PMID:10799292] |
| 3. Wiemann, S S and 23 more authors.. (2001) Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.. Genome research, [PMID:11230166] |
| 4. Lee, J H JH, Voo, K S KS and Skalnik, D G DG.. (2001) Identification and characterization of the DNA binding domain of CpG-binding protein.. The Journal of biological chemistry, (30): [PMID:11572867] |
| 5. Lee, Jeong-Heon JH and Skalnik, David G DG.. (2002) CpG-binding protein is a nuclear matrix- and euchromatin-associated protein localized to nuclear speckles containing human trithorax. Identification of nuclear matrix targeting signals.. The Journal of biological chemistry, (1): [PMID:12200428] |
| 6. Nusbaum, Chad C and 54 more authors.. (2005) DNA sequence and analysis of human chromosome 18.. Nature, (22): [PMID:16177791] |
| 7. Lee, Jeong-Heon JH and Skalnik, David G DG.. (2005) CpG-binding protein (CXXC finger protein 1) is a component of the mammalian Set1 histone H3-Lys4 methyltransferase complex, the analogue of the yeast Set1/COMPASS complex.. The Journal of biological chemistry, (16): [PMID:16253997] |
| 8. Lee, Jeong-Heon JH, Tate, Courtney M CM, You, Jin-Sam JS and Skalnik, David G DG.. (2007) Identification and characterization of the human Set1B histone H3-Lys4 methyltransferase complex.. The Journal of biological chemistry, (4): [PMID:17355966] |
| 9. Lee, Jeong-Heon JH and Skalnik, David G DG.. (2008) Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes.. Molecular and cellular biology, [PMID:17998332] |
| 10. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 11. Wu, Min M and 6 more authors.. (2008) Molecular regulation of H3K4 trimethylation by Wdr82, a component of human Set1/COMPASS.. Molecular and cellular biology, [PMID:18838538] |
| 12. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 13. Xu, Chao C, Bian, Chuanbing C, Lam, Robert R, Dong, Aiping A and Min, Jinrong J.. (2011) The structural basis for selective binding of non-methylated CpG islands by the CFP1 CXXC domain.. Nature communications, [PMID:21407193] |
| 14. Yang, Yawei J YJ and 17 more authors.. (2012) Microcephaly gene links trithorax and REST/NRSF to control neural stem cell proliferation and differentiation.. Cell, (21): [PMID:23178126] |
| 15. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
