| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab096509-10μl | 10μl | In stock | $66.90 | |
| Ab096509-50μl | 50μl | In stock | $189.90 | |
| Ab096509-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab096509-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human cIAP1 Antibody; WB, IF, ICC; Unconjugated
| Product Name | Recombinant cIAP1 Antibody - Primary antibody, specific to BIRC2, Rabbit IgG |
|---|---|
| Synonyms | API 1 antibody | API1 antibody | Apoptosis inhibitor 1 antibody | Baculoviral IAP repeat containing 2 antibody | Baculoviral IAP repeat containing protein 2 antibody | Baculoviral IAP repeat-containing protein 2 antibody | BIRC 2 antibody | BIRC2 antibody |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | BIRC2 |
| Immunogen | A synthetic peptide derived from human cIAP1 (AA 29-42). |
| Positive Control | WB: HeLa, HUVEC, HT-29 and LNCaP cell lysates. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human cIAP1 Antibody, Recombinant, could be used for WB, IF/ICC and so on. Protein Function: |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant cIAP1 Antibody (Recombinant cIAP1 Antibody (Ab096509) - Western Blot
All lanes: Recombinant cIAP1 Antibody (Ab096509) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 70 kDa
Observed band size: 65 kDa
Exposure time: 284.5 s
Recombinant cIAP1 Antibody (Ab096509) - IF/ICC
Immunocytochemistry analysis of Ciap1 (green) in HeLa using Recombinant cIAP1 Antibody (Ab096509), and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC | 1/50 - 1/200 "> 1/50 - 1/200 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303170 | Certificate of Analysis | Mar 13, 2024 | Ab096509 |
| ZJ24F0303169 | Certificate of Analysis | Mar 13, 2024 | Ab096509 |
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| 2. Allensworth JL, Sauer SJ, Lyerly HK, Morse MA, Devi GR. (2013) Smac mimetic Birinapant induces apoptosis and enhances TRAIL potency in inflammatory breast cancer cells in an IAP-dependent and TNF-α-independent mechanism.. Breast Cancer Res Treat, 137 (2): (359-71). [PMID:23225169] [10.1021/op500134e] |
| 3. Cai Q, Sun H, Peng Y, Lu J, Nikolovska-Coleska Z, McEachern D, Liu L, Qiu S, Yang CY, Miller R et al.. (2011) A potent and orally active antagonist (SM-406/AT-406) of multiple inhibitor of apoptosis proteins (IAPs) in clinical development for cancer treatment.. J Med Chem, 54 (8): (2714-26). [PMID:21443232] [10.1021/op500134e] |
| 4. Flygare JA, Beresini M, Budha N, Chan H, Chan IT, Cheeti S, Cohen F, Deshayes K, Doerner K, Eckhardt SG et al.. (2012) Discovery of a potent small-molecule antagonist of inhibitor of apoptosis (IAP) proteins and clinical candidate for the treatment of cancer (GDC-0152).. J Med Chem, 55 (9): (4101-13). [PMID:22413863] [10.1021/op500134e] |
| 5. Donnell AF, Michoud C, Rupert KC, Han X, Aguilar D, Frank KB, Fretland AJ, Gao L, Goggin B, Hogg JH et al.. (2013) Benzazepinones and benzoxazepinones as antagonists of inhibitor of apoptosis proteins (IAPs) selective for the second baculovirus IAP repeat (BIR2) domain.. J Med Chem, 56 (20): (7772-87). [PMID:24083782] [10.1021/op500134e] |
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| 7. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811] |
| 8. Zhang B, Nikolovska-Coleska Z, Zhang Y, Bai L, Qiu S, Yang CY, Sun H, Wang S, Wu Y.. (2008) Design, synthesis, and evaluation of tricyclic, conformationally constrained small-molecule mimetics of second mitochondria-derived activator of caspases.. J Med Chem, 51 (23): (7352-7355). [PMID:19012392] [10.1021/jm801146d] |
| 9. Sun W, Nikolovska-Coleska Z, Qin D, Sun H, Yang CY, Bai L, Qiu S, Wang Y, Ma D, Wang S.. (2009) Design, synthesis, and evaluation of potent, nonpeptidic mimetics of second mitochondria-derived activator of caspases.. J Med Chem, 52 (3): (593-596). [PMID:19138149] [10.1021/jm801101z] |
| 10. Cohen F, Alicke B, Elliott LO, Flygare JA, Goncharov T, Keteltas SF, Franklin MC, Frankovitz S, Stephan JP, Tsui V, Vucic D, Wong H, Fairbrother WJ.. (2009) Orally bioavailable antagonists of inhibitor of apoptosis proteins based on an azabicyclooctane scaffold.. J Med Chem, 52 (6): (1723-1730). [PMID:19228017] [10.1021/jm801450c] |
| 11. Sun H, Liu L, Lu J, Qiu S, Yang CY, Yi H, Wang S.. (2010) Cyclopeptide Smac mimetics as antagonists of IAP proteins.. Bioorg Med Chem Lett, 20 (10): (3043-3046). [PMID:20443226] [10.1016/j.bmcl.2010.03.114] |
| 12. Sun H, Liu L, Lu J, Bai L, Li X, Nikolovska-Coleska Z, McEachern D, Yang CY, Qiu S, Yi H, Sun D, Wang S.. (2011) Potent bivalent Smac mimetics: effect of the linker on binding to inhibitor of apoptosis proteins (IAPs) and anticancer activity.. J Med Chem, 54 (9): (3306-3318). [PMID:21462933] [10.1021/jm101651b] |
| 13. Sheng R, Sun H, Liu L, Lu J, McEachern D, Wang G, Wen J, Min P, Du Z, Lu H, Kang S, Guo M, Yang D, Wang S.. (2013) A potent bivalent Smac mimetic (SM-1200) achieving rapid, complete, and durable tumor regression in mice.. J Med Chem, 56 (10): (3969-3979). [PMID:23651223] [10.1021/jm400216d] |
| 14. Kester RF, Donnell AF, Lou Y, Remiszewski SW, Lombardo LJ, Chen S, Le NT, Lo J, Moliterni JA, Han X, Hogg JH, Liang W, Michoud C, Rupert KC, Mischke S, Le K, Weisel M, Janson CA, Lukacs CM, Fretland AJ, Hong K, Polonskaia A, Gao L, Li S, Solis DS, Aguilar D, Tardell C, Dvorozniak M, Tannu S, Lee EC, Schutt AD, Goggin B.. (2013) Optimization of benzodiazepinones as selective inhibitors of the X-linked inhibitor of apoptosis protein (XIAP) second baculovirus IAP repeat (BIR2) domain.. J Med Chem, 56 (20): (7788-7803). [PMID:24093940] [10.1021/jm400732v] |
| 15. Perez HL, Chaudhry C, Emanuel SL, Fanslau C, Fargnoli J, Gan J, Kim KS, Lei M, Naglich JG, Traeger SC, Vuppugalla R, Wei DD, Vite GD, Talbott RL, Borzilleri RM.. (2015) Discovery of potent heterodimeric antagonists of inhibitor of apoptosis proteins (IAPs) with sustained antitumor activity.. J Med Chem, 58 (3): (1556-1562). [PMID:25584393] [10.1021/jm501482t] |
| 16. Demizu Y, Shibata N, Hattori T, Ohoka N, Motoi H, Misawa T, Shoda T, Naito M, Kurihara M.. (2016) Development of BCR-ABL degradation inducers via the conjugation of an imatinib derivative and a cIAP1 ligand.. Bioorg Med Chem Lett, 26 (20): (4865-4869). [PMID:27666635] [10.1016/j.bmcl.2016.09.041] |
| 17. Tamanini E, Buck IM, Chessari G, Chiarparin E, Day JEH, Frederickson M, Griffiths-Jones CM, Hearn K, Heightman TD, Iqbal A, Johnson CN, Lewis EJ, Martins V, Peakman T, Reader M, Rich SJ, Ward GA, Williams PA, Wilsher NE.. (2017) Discovery of a Potent Nonpeptidomimetic, Small-Molecule Antagonist of Cellular Inhibitor of Apoptosis Protein 1 (cIAP1) and X-Linked Inhibitor of Apoptosis Protein (XIAP).. J Med Chem, 60 (11): (4611-4625). [PMID:28492317] [10.1021/acs.jmedchem.6b01877] |
| 18. Shibata N, Nagai K, Morita Y, Ujikawa O, Ohoka N, Hattori T, Koyama R, Sano O, Imaeda Y, Nara H, Cho N, Naito M.. (2018) Development of Protein Degradation Inducers of Androgen Receptor by Conjugation of Androgen Receptor Ligands and Inhibitor of Apoptosis Protein Ligands.. J Med Chem, 61 (2): (543-575). [PMID:28594553] [10.1021/acs.jmedchem.7b00168] |
| 19. Baggio C, Gambini L, Udompholkul P, Salem AF, Aronson A, Dona A, Troadec E, Pichiorri F, Pellecchia M.. (2018) Design of Potent pan-IAP and Lys-Covalent XIAP Selective Inhibitors Using a Thermodynamics Driven Approach.. J Med Chem, 61 (14): (6350-6363). [PMID:29940121] [10.1021/acs.jmedchem.8b00810] |
| 20. Yokoo H,Shibata N,Naganuma M,Murakami Y,Fujii K,Ito T,Aritake K,Naito M,Demizu Y. (2021) Development of a Hematopoietic Prostaglandin D Synthase-Degradation Inducer.. ACS Med Chem Lett, 12 (2): (236-241). [PMID:33603969] [10.1021/acsmedchemlett.0c00605] |
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| 22. Liston, P P and 10 more authors.. (1996) Suppression of apoptosis in mammalian cells by NAIP and a related family of IAP genes.. Nature, (25): [PMID:8552191] |
| 23. Uren, A G AG, Pakusch, M M, Hawkins, C J CJ, Puls, K L KL and Vaux, D L DL.. (1996) Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors.. Proceedings of the National Academy of Sciences of the United States of America, (14): [PMID:8643514] |
| 24. Hinds, M G MG, Norton, R S RS, Vaux, D L DL and Day, C L CL.. (1999) Solution structure of a baculoviral inhibitor of apoptosis (IAP) repeat.. Nature structural biology, [PMID:10404221] |
| 25. Samuel, Temesgen T and 5 more authors.. (2005) cIAP1 Localizes to the nuclear compartment and modulates the cell cycle.. Cancer research, (1): [PMID:15665297] |
| 26. Zobel, Kerry K and 10 more authors.. (2006) Design, synthesis, and biological activity of a potent Smac mimetic that sensitizes cancer cells to apoptosis by antagonizing IAPs.. ACS chemical biology, (19): [PMID:17168540] |
| 27. Xu, Lei L and 7 more authors.. (2007) c-IAP1 cooperates with Myc by acting as a ubiquitin ligase for Mad1.. Molecular cell, (14): [PMID:18082613] |
| 28. Dubrez-Daloz, Laurence L, Dupoux, Alban A and Cartier, Jessy J.. (2008) IAPs: more than just inhibitors of apoptosis proteins.. Cell cycle (Georgetown, Tex.), (15): [PMID:18414036] |
| 29. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 30. Sun, M M, Song, L L, Li, Y Y, Zhou, T T and Jope, R S RS.. (2008) Identification of an antiapoptotic protein complex at death receptors.. Cell death and differentiation, [PMID:18846110] |
| 31. Kulathila, Raviraj R and 8 more authors.. (2009) The structure of the BIR3 domain of cIAP1 in complex with the N-terminal peptides of SMAC and caspase-9.. Acta crystallographica. Section D, Biological crystallography, [PMID:19153467] |
| 32. Ndubaku, Chudi C and 15 more authors.. (2009) Antagonism of c-IAP and XIAP proteins is required for efficient induction of cell death by small-molecule IAP antagonists.. ACS chemical biology, (17): [PMID:19492850] |
| 33. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 34. Lopez, Juanita J and Meier, Pascal P.. (2010) To fight or die - inhibitor of apoptosis proteins at the crossroad of innate immunity and death.. Current opinion in cell biology, [PMID:20888210] |
| 35. Damgaard, Rune B RB and Gyrd-Hansen, Mads M.. (2011) Inhibitor of apoptosis (IAP) proteins in regulation of inflammation and innate immunity.. Discovery medicine, [PMID:21447281] |
| 36. Lopez, Juanita J and 10 more authors.. (2011) CARD-mediated autoinhibition of cIAP1's E3 ligase activity suppresses cell proliferation and migration.. Molecular cell, (10): [PMID:21549626] |
| 37. Cartier, Jessy J and 12 more authors.. (2011) Cellular inhibitor of apoptosis protein-1 (cIAP1) can regulate E2F1 transcription factor-mediated control of cyclin transcription.. The Journal of biological chemistry, (29): [PMID:21653699] |
| 38. Mei, Yide Y, Hahn, Allison Alcivar AA, Hu, Shimin S and Yang, Xiaolu X.. (2011) The USP19 deubiquitinase regulates the stability of c-IAP1 and c-IAP2.. The Journal of biological chemistry, (14): [PMID:21849505] |
| 39. Bertrand, Mathieu J M MJ and 8 more authors.. (2011) cIAP1/2 are direct E3 ligases conjugating diverse types of ubiquitin chains to receptor interacting proteins kinases 1 to 4 (RIP1-4).. PloS one, [PMID:21931591] |
| 40. Darding, M M and Meier, P P.. (2012) IAPs: guardians of RIPK1.. Cell death and differentiation, [PMID:22095281] |
| 41. Zhou, Alicia Y AY and 6 more authors.. (2013) IKKε-mediated tumorigenesis requires K63-linked polyubiquitination by a cIAP1/cIAP2/TRAF2 E3 ubiquitin ligase complex.. Cell reports, (28): [PMID:23453969] |
| 42. Synoradzki, Kamil K and Bieganowski, Pawel P.. (2015) Middle domain of human Hsp90 isoforms differentially binds Aha1 in human cells and alters Hsp90 activity in yeast.. Biochimica et biophysica acta, [PMID:25486457] |
| 43. Wang, Yu-Bo YB and 13 more authors.. (2015) Ubiquitin-associated domain-containing ubiquitin regulatory X (UBX) protein UBXN1 is a negative regulator of nuclear factor κB (NF-κB) signaling.. The Journal of biological chemistry, (17): [PMID:25681446] |
| 44. Anderson NA, Cryan J, Ahmed A, Dai H, McGonagle GA, Rozier C, Benowitz AB.. (2020) Selective CDK6 degradation mediated by cereblon, VHL, and novel IAP-recruiting PROTACs.. Bioorg Med Chem Lett, 30 (9): (127106-127106). [PMID:32184044] [10.1016/j.bmcl.2020.127106] |
