| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab096857-10μl | 10μl | In stock | $69.90 | |
| Ab096857-50μl | 50μl | In stock | $199.90 | |
| Ab096857-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab096857-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human CLIC4 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Recombinant CLIC4 Antibody - Primary antibody, specific to CLIC4, Rabbit IgG |
|---|---|
| Synonyms | MC3S5 | Chloride intracellular channel 4 | Chloride intracellular channel 4 (mitochondrial) | Chloride intracellular channel 4 like | Chloride intracellular channel protein 4 | Clic4 | CLIC4_HUMAN | CLIC4L | DKFZP566G223 | FLJ38640 | H1 antibody HUH1 | In |
| Specifications & Purity | Recombinant, ExactAb™, Validated, KD, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | CLIC4 |
| Immunogen | A synthetic peptide derived from human CLIC4 (AA 3-16) |
| Positive Control | WB: HeLa, HEK293, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human Cholangiocarcinoma tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, KD Validation, Recombinant, Validated |
| Product Description |
Rabbit anti Human CLIC4 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant CLIC4 Antibody (Ab096857) - Western Blot
All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 28 kDa
Exposure time: 21.9 s
Recombinant CLIC4 Antibody (Ab096857) - Western Blot
All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 28 kDa
Exposure time: 35.0 s
Recombinant CLIC4 Antibody (Ab096857) - IF/ICC
Immunocytochemistry analysis of CLIC4 (green) in HeLa using Recombinant CLIC4 Antibody (Ab096857), and cells were counterstained with DAPI (blue).
Recombinant CLIC4 Antibody (Ab096857) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant CLIC4 Antibody (Ab096857). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant CLIC4 Antibody (Ab096857) - Western Blot
All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 28 kDa
Exposure time: 0.5 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303176 | Certificate of Analysis | Mar 13, 2024 | Ab096857 |
| ZJ24F0303175 | Certificate of Analysis | Mar 13, 2024 | Ab096857 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. and Edwards, J C JC.. (1999) A novel p64-related Cl- channel: subcellular distribution and nephron segment-specific expression.. The American journal of physiology, [PMID:10070163] |
| 4. Chuang, J Z JZ, Milner, T A TA, Zhu, M M and Sung, C H CH.. (1999) A 29 kDa intracellular chloride channel p64H1 is associated with large dense-core vesicles in rat hippocampal neurons.. The Journal of neuroscience : the official journal of the Society for Neuroscience, (15): [PMID:10191309] |
| 5. Berryman, M M and Bretscher, A A.. (2000) Identification of a novel member of the chloride intracellular channel gene family (CLIC5) that associates with the actin cytoskeleton of placental microvilli.. Molecular biology of the cell, [PMID:10793131] |
| 6. Wiemann, S S and 23 more authors.. (2001) Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.. Genome research, [PMID:11230166] |
| 7. Rønnov-Jessen, Lone L, Villadsen, René R, Edwards, John C JC and Petersen, Ole W OW.. (2002) Differential expression of a chloride intracellular channel gene, CLIC4, in transforming growth factor-beta1-mediated conversion of fibroblasts to myofibroblasts.. The American journal of pathology, [PMID:12163372] |
| 8. Shanks, Ryan A RA and 6 more authors.. (2002) AKAP350 at the Golgi apparatus. II. Association of AKAP350 with a novel chloride intracellular channel (CLIC) family member.. The Journal of biological chemistry, (25): [PMID:12163479] |
| 9. Berryman, Mark A MA and Goldenring, James R JR.. (2003) CLIC4 is enriched at cell-cell junctions and colocalizes with AKAP350 at the centrosome and midbody of cultured mammalian cells.. Cell motility and the cytoskeleton, [PMID:14569596] |
| 10. Littler, Dene R DR and 10 more authors.. (2005) Crystal structure of the soluble form of the redox-regulated chloride ion channel protein CLIC4.. The FEBS journal, [PMID:16176272] |
| 11. Bohman, Svante S and 6 more authors.. (2005) Proteomic analysis of vascular endothelial growth factor-induced endothelial cell differentiation reveals a role for chloride intracellular channel 4 (CLIC4) in tubular morphogenesis.. The Journal of biological chemistry, (23): [PMID:16239224] |
| 12. Suh, Kwang S KS and 8 more authors.. (2007) Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers.. Clinical cancer research : an official journal of the American Association for Cancer Research, (1): [PMID:17200346] |
| 13. Suh, Kwang S KS and 8 more authors.. (2007) CLIC4 mediates and is required for Ca2+-induced keratinocyte differentiation.. Journal of cell science, (1): [PMID:17636002] |
| 14. Tung, Jennifer J JJ, Hobert, Oliver O, Berryman, Mark M and Kitajewski, Jan J.. (2009) Chloride intracellular channel 4 is involved in endothelial proliferation and morphogenesis in vitro.. Angiogenesis, [PMID:19247789] |
| 15. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 16. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 17. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 18. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
