Recombinant CLIC4 Antibody - Primary antibody, specific to CLIC4, Rabbit IgG

Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human CLIC4 Antibody; WB, IHC, IF, ICC; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • IF/ICC
  • IHC
  • WB
Item Number
Ab096857
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab096857-10μl
10μl
In stock
$69.90
Ab096857-50μl
50μl
In stock
$199.90
Ab096857-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab096857-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human CLIC4 Antibody; WB, IHC, IF, ICC; Unconjugated

Basic Description

Product NameRecombinant CLIC4 Antibody - Primary antibody, specific to CLIC4, Rabbit IgG
SynonymsMC3S5 | Chloride intracellular channel 4 | Chloride intracellular channel 4 (mitochondrial) | Chloride intracellular channel 4 like | Chloride intracellular channel protein 4 | Clic4 | CLIC4_HUMAN | CLIC4L | DKFZP566G223 | FLJ38640 | H1 antibody HUH1 | In
Specifications & PurityRecombinant, ExactAb™, Validated, KD, 0.5 mg/mL
Host speciesRabbit
SpecificityCLIC4
ImmunogenA synthetic peptide derived from human CLIC4 (AA 3-16)
Positive ControlWB: HeLa, HEK293, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human Cholangiocarcinoma tissue. ICC/IF: HeLa cells.
ConjugationUnconjugated
GradeExactAb™, KD Validation, Recombinant, Validated
Product Description

Rabbit anti Human CLIC4 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant CLIC4 Antibody (Ab096857) - Western Blot
All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 29 kDa
Observed band size: 28 kDa
Exposure time: 21.9 s

Recombinant CLIC4 Antibody (Ab096857) - Western Blot
All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 29 kDa
Observed band size: 28 kDa
Exposure time: 35.0 s

Recombinant CLIC4 Antibody (Ab096857) - IF/ICC
Immunocytochemistry analysis of CLIC4 (green) in HeLa using Recombinant CLIC4 Antibody (Ab096857), and cells were counterstained with DAPI (blue). 

Recombinant CLIC4 Antibody (Ab096857) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant CLIC4 Antibody (Ab096857). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Recombinant CLIC4 Antibody (Ab096857) - Western Blot
All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 29 kDa
Observed band size: 28 kDa
Exposure time: 0.5 s

Associated Targets(Human)

CLIC4 Tbio Chloride intracellular channel protein 4 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

ICC/IF

1/50-1/200

IHC

1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303176Certificate of AnalysisMar 13, 2024 Ab096857
ZJ24F0303175Certificate of AnalysisMar 13, 2024 Ab096857

Related Documents

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861]
2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
3. and Edwards, J C JC..  (1999)  A novel p64-related Cl- channel: subcellular distribution and nephron segment-specific expression..  The American journal of physiology,      [PMID:10070163]
4. Chuang, J Z JZ, Milner, T A TA, Zhu, M M and Sung, C H CH..  (1999)  A 29 kDa intracellular chloride channel p64H1 is associated with large dense-core vesicles in rat hippocampal neurons..  The Journal of neuroscience : the official journal of the Society for Neuroscience,    (15):   [PMID:10191309]
5. Berryman, M M and Bretscher, A A..  (2000)  Identification of a novel member of the chloride intracellular channel gene family (CLIC5) that associates with the actin cytoskeleton of placental microvilli..  Molecular biology of the cell,      [PMID:10793131]
6. Wiemann, S S and 23 more authors..  (2001)  Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs..  Genome research,      [PMID:11230166]
7. Rønnov-Jessen, Lone L, Villadsen, René R, Edwards, John C JC and Petersen, Ole W OW..  (2002)  Differential expression of a chloride intracellular channel gene, CLIC4, in transforming growth factor-beta1-mediated conversion of fibroblasts to myofibroblasts..  The American journal of pathology,      [PMID:12163372]
8. Shanks, Ryan A RA and 6 more authors..  (2002)  AKAP350 at the Golgi apparatus. II. Association of AKAP350 with a novel chloride intracellular channel (CLIC) family member..  The Journal of biological chemistry,    (25):   [PMID:12163479]
9. Berryman, Mark A MA and Goldenring, James R JR..  (2003)  CLIC4 is enriched at cell-cell junctions and colocalizes with AKAP350 at the centrosome and midbody of cultured mammalian cells..  Cell motility and the cytoskeleton,      [PMID:14569596]
10. Littler, Dene R DR and 10 more authors..  (2005)  Crystal structure of the soluble form of the redox-regulated chloride ion channel protein CLIC4..  The FEBS journal,      [PMID:16176272]
11. Bohman, Svante S and 6 more authors..  (2005)  Proteomic analysis of vascular endothelial growth factor-induced endothelial cell differentiation reveals a role for chloride intracellular channel 4 (CLIC4) in tubular morphogenesis..  The Journal of biological chemistry,    (23):   [PMID:16239224]
12. Suh, Kwang S KS and 8 more authors..  (2007)  Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers..  Clinical cancer research : an official journal of the American Association for Cancer Research,    (1):   [PMID:17200346]
13. Suh, Kwang S KS and 8 more authors..  (2007)  CLIC4 mediates and is required for Ca2+-induced keratinocyte differentiation..  Journal of cell science,    (1):   [PMID:17636002]
14. Tung, Jennifer J JJ, Hobert, Oliver O, Berryman, Mark M and Kitajewski, Jan J..  (2009)  Chloride intracellular channel 4 is involved in endothelial proliferation and morphogenesis in vitro..  Angiogenesis,      [PMID:19247789]
15. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,    (15):   [PMID:21406692]
16. Bienvenut, Willy V WV and 6 more authors..  (2012)  Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features..  Molecular & cellular proteomics : MCP,      [PMID:22223895]
17. Bian, Yangyang Y and 9 more authors..  (2014)  An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome..  Journal of proteomics,    (16):   [PMID:24275569]
18. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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