Recombinant CLP1 Antibody - Primary antibody, specific to CLP1, Rabbit IgG
Synonyms
Cleavage and polyadenylation factor I subunit 1 antibody | CLP 1 antibody | clp1 antibody | CLP1 cleavage and polyadenylation factor I subunit homolog antibody | CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae) antibody | CLP1,
Specifications & Purity
ExactAb™, Validated, KD, Recombinant, Lot by Lot
Host species
Rabbit
Specificity
CLP1
Immunogen
A synthetic peptide derived from human CLP1 (AA 1-25).
Positive Control
WB: HeLa, Hep G2, HEK293 and Jurkat cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, KD Validation, Recombinant, Validated
Product Description
Rabbit anti Human CLP1 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/2000 Protein Function: Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant CLP1 Antibody (Ab096908) - Western Blot All lanes: Recombinant CLP1 Antibody (Ab096908) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 48 kDa Observed band size: 43 kDa Exposure time: 200.0 s