Grouped product items

SKU

Size

Availability

Price

Qty

Ab096938-10μl

10μl

In stock

$66.90

Ab096938-50μl

50μl

In stock

$199.90

Ab096938-100μl

100μl

Available within 8-12 weeks(?)

$299.90

Ab096938-1ml

1ml

Available within 8-12 weeks(?)

$2,499.90

Basic Description

Product Name	Recombinant Clusterin Antibody - Primary antibody, specific to Clusterin, Rabbit IgG
Synonyms	40 antibody \| AAG 4 antibody \| AAG4 antibody \| Aging associated protein 4 antibody \| Aging-associated gene 4 protein antibody \| AI893575 antibody \| APO J antibody \| Apo-J antibody \| APOJ antibody \| ApoJalpha antibody \| ApoJbeta antibody \| Apolipoprotein J
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.1 mg/mL
Host species	Rabbit
Specificity	Clusterin
Immunogen	A synthetic peptide derived from human Clusterin (AA 400-449)
Positive Control	WB: Human serum, U-2 OS and HeLa lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human Clusterin Antibody, Recombinant, could be used for WB and so on. Application WB: 1/500-1/1000 Protein Function Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	0.1 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Clusterin Antibody (Ab096938) - Western Blot
All lanes: Recombinant Clusterin Antibody (Ab096938) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 37, 66 kDa
Exposure time: 32.1 s

Associated Targets(Human)

CLU Tbio Clusterin (0 Activities)

Discover Target: CLU

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0505743	Certificate of Analysis	May 16, 2024	Ab096938
ZJ24F0505742	Certificate of Analysis	May 16, 2024	Ab096938

FAQs and Articles

FAQ

Protocol of Western Blotting For High Molecular Weights

Categories: Protocols

Tags:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

References

1. Choi-Miura, N H NH, Takahashi, Y Y, Nakano, Y Y, Tobe, T T and Tomita, M M.. (1992) Identification of the disulfide bonds in human plasma protein SP-40,40 (apolipoprotein-J).. Journal of biochemistry, [PMID:1491011]
2. Kirszbaum, L L, Bozas, S E SE and Walker, I D ID.. (1992) SP-40,40, a protein involved in the control of the complement pathway, possesses a unique array of disulphide bridges.. FEBS letters, (3): [PMID:1551440]
3. James, R W RW and 5 more authors.. () Characterization of a human high density lipoprotein-associated protein, NA1/NA2. Identity with SP-40,40, an inhibitor of complement-mediated cytolysis.. Arteriosclerosis and thrombosis : a journal of vascular biology, [PMID:1903064]
4. Danik, M M and 6 more authors.. (1991) Human gliomas and epileptic foci express high levels of a mRNA related to rat testicular sulfated glycoprotein 2, a purported marker of cell death.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1924317]
5. de Silva, H V HV, Harmony, J A JA, Stuart, W D WD, Gil, C M CM and Robbins, J J.. (1990) Apolipoprotein J: structure and tissue distribution.. Biochemistry, (5): [PMID:1974459]
6. de Silva, H V HV and 7 more authors.. (1990) Purification and characterization of apolipoprotein J.. The Journal of biological chemistry, (25): [PMID:2387851]
7. Choi, N H NH, Mazda, T T and Tomita, M M.. (1989) A serum protein SP40,40 modulates the formation of membrane attack complex of complement on erythrocytes.. Molecular immunology, [PMID:2601725]
8. Kirszbaum, L L and 6 more authors.. (1989) Molecular cloning and characterization of the novel, human complement-associated protein, SP-40,40: a link between the complement and reproductive systems.. The EMBO journal, [PMID:2721499]
9. Jenne, D E DE and Tschopp, J J.. (1989) Molecular structure and functional characterization of a human complement cytolysis inhibitor found in blood and seminal plasma: identity to sulfated glycoprotein 2, a constituent of rat testis fluid.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2780565]
10. Hochstrasser, A C AC and 6 more authors.. (1988) HDL particle associated proteins in plasma and cerebrospinal fluid: identification and partial sequencing.. Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society, [PMID:3154963]
11. Wong, P P and 5 more authors.. (1994) Molecular characterization of human TRPM-2/clusterin, a gene associated with sperm maturation, apoptosis and neurodegeneration.. European journal of biochemistry, (1): [PMID:8181474]
12. Kelso, G J GJ and 5 more authors.. (1994) Apolipoprotein J is associated with paraoxonase in human plasma.. Biochemistry, (25): [PMID:8292612]
13. Ghiso, J J and 6 more authors.. (1993) The cerebrospinal-fluid soluble form of Alzheimer's amyloid beta is complexed to SP-40,40 (apolipoprotein J), an inhibitor of the complement membrane-attack complex.. The Biochemical journal, (1): [PMID:8328966]
14. Reddy, K B KB, Karode, M C MC, Harmony, A K AK and Howe, P H PH.. (1996) Interaction of transforming growth factor beta receptors with apolipoprotein J/clusterin.. Biochemistry, (9): [PMID:8555189]
15. Kapron, J T JT and 6 more authors.. (1997) Identification and characterization of glycosylation sites in human serum clusterin.. Protein science : a publication of the Protein Society, [PMID:9336835]
16. Poon, S S, Easterbrook-Smith, S B SB, Rybchyn, M S MS, Carver, J A JA and Wilson, M R MR.. (2000) Clusterin is an ATP-independent chaperone with very broad substrate specificity that stabilizes stressed proteins in a folding-competent state.. Biochemistry, (26): [PMID:11123922]
17. Hatters, Danny M DM, Wilson, Mark R MR, Easterbrook-Smith, Simon B SB and Howlett, Geoffrey J GJ.. (2002) Suppression of apolipoprotein C-II amyloid formation by the extracellular chaperone, clusterin.. European journal of biochemistry, [PMID:12047389]
18. Poon, Stephen S and 5 more authors.. (2002) Mildly acidic pH activates the extracellular molecular chaperone clusterin.. The Journal of biological chemistry, (18): [PMID:12176985]
19. Leskov, Konstantin S KS, Klokov, Dmitry Y DY, Li, Jing J, Kinsella, Timothy J TJ and Boothman, David A DA.. (2003) Synthesis and functional analyses of nuclear clusterin, a cell death protein.. The Journal of biological chemistry, (28): [PMID:12551933]
20. Zhang, Hui H, Li, Xiao-Jun XJ, Martin, Daniel B DB and Aebersold, Ruedi R.. (2003) Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.. Nature biotechnology, [PMID:12754519]
21. Santilli, Giorgia G, Aronow, Bruce J BJ and Sala, Arturo A.. (2003) Essential requirement of apolipoprotein J (clusterin) signaling for IkappaB expression and regulation of NF-kappaB activity.. The Journal of biological chemistry, (3): [PMID:12882985]
22. Bunkenborg, Jakob J, Pilch, Bartosz J BJ, Podtelejnikov, Alexandre V AV and Wiśniewski, Jacek R JR.. (2004) Screening for N-glycosylated proteins by liquid chromatography mass spectrometry.. Proteomics, [PMID:14760718]
23. Takahashi, Meiko M, Lin, Yu-Min YM, Nakamura, Yusuke Y and Furukawa, Yoichi Y.. (2004) Isolation and characterization of a novel gene CLUAP1 whose expression is frequently upregulated in colon cancer.. Oncogene, (9): [PMID:15480429]
24. Zhang, Honglai H and 5 more authors.. (2005) Clusterin inhibits apoptosis by interacting with activated Bax.. Nature cell biology, [PMID:16113678]
25. Lewandrowski, Urs U, Moebius, Jan J, Walter, Ulrich U and Sickmann, Albert A.. (2006) Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach.. Molecular & cellular proteomics : MCP, [PMID:16263699]
26. Liu, Tao T and 6 more authors.. () Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952]
27. Nusbaum, Chad C and 75 more authors.. (2006) DNA sequence and analysis of human chromosome 8.. Nature, (19): [PMID:16421571]
28. Leong, Wai Fook WF and Chow, Vincent T K VT.. (2006) Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection.. Cellular microbiology, [PMID:16548883]
29. Ramachandran, Prasanna P and 5 more authors.. (2006) Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry.. Journal of proteome research, [PMID:16740002]
30. Trougakos, Ioannis P IP, Pawelec, Graham G, Tzavelas, Christos C, Ntouroupi, Triantaphillia T and Gonos, Efstathios S ES.. () Clusterin/Apolipoprotein J up-regulation after zinc exposure, replicative senescence or differentiation of human haematopoietic cells.. Biogerontology, [PMID:16955214]
31. Cochrane, Dawn R DR, Wang, Zhou Z, Muramaki, Motosugu M, Gleave, Martin E ME and Nelson, Colleen C CC.. (2007) Differential regulation of clusterin and its isoforms by androgens in prostate cells.. The Journal of biological chemistry, (26): [PMID:17148459]
32. Stewart, Elise M EM and 6 more authors.. (2007) Effects of glycosylation on the structure and function of the extracellular chaperone clusterin.. Biochemistry, (6): [PMID:17260971]
33. Andersen, Claus Lindbjerg CL and 7 more authors.. (2007) Clusterin expression in normal mucosa and colorectal cancer.. Molecular & cellular proteomics : MCP, [PMID:17322305]
34. Kumita, Janet R JR and 9 more authors.. (2007) The extracellular chaperone clusterin potently inhibits human lysozyme amyloid formation by interacting with prefibrillar species.. Journal of molecular biology, (25): [PMID:17407782]
35. Yerbury, Justin J JJ and 6 more authors.. (2007) The extracellular chaperone clusterin influences amyloid formation and toxicity by interacting with prefibrillar structures.. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, [PMID:17412999]
36. Nizard, Philippe P and 6 more authors.. (2007) Stress-induced retrotranslocation of clusterin/ApoJ into the cytosol.. Traffic (Copenhagen, Denmark), [PMID:17451556]
37. Wang, Zengjun Z, Widgren, Esther E EE, Richardson, Richard T RT and O'Rand, Michael G MG.. (2007) Characterization of an eppin protein complex from human semen and spermatozoa.. Biology of reproduction, [PMID:17567961]
38. Ranney, Melissa K MK, Ahmed, Ikhlas S A IS, Potts, Kelly R KR and Craven, Rolf J RJ.. (2007) Multiple pathways regulating the anti-apoptotic protein clusterin in breast cancer.. Biochimica et biophysica acta, [PMID:17689225]
39. Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31): [PMID:17974005]
40. Rizzi, Federica F, Caccamo, Alessandro Emanuele AE, Belloni, Lucia L and Bettuzzi, Saverio S.. (2009) Clusterin is a short half-life, poly-ubiquitinated protein, which controls the fate of prostate cancer cells.. Journal of cellular physiology, [PMID:19137541]
41. Wyatt, Amy R AR, Yerbury, Justin J JJ and Wilson, Mark R MR.. (2009) Structural characterization of clusterin-chaperone client protein complexes.. The Journal of biological chemistry, (14): [PMID:19535339]
42. Wyatt, Amy R AR and Wilson, Mark R MR.. (2010) Identification of human plasma proteins as major clients for the extracellular chaperone clusterin.. The Journal of biological chemistry, (5): [PMID:19996109]
43. Zoubeidi, Amina A and 8 more authors.. (2010) Clusterin facilitates COMMD1 and I-kappaB degradation to enhance NF-kappaB activity in prostate cancer cells.. Molecular cancer research : MCR, [PMID:20068069]
44. Wyatt, Amy R AR and 6 more authors.. (2011) Clusterin facilitates in vivo clearance of extracellular misfolded proteins.. Cellular and molecular life sciences : CMLS, [PMID:21505792]
45. Kim, Nayoung N and 13 more authors.. (2012) Human nuclear clusterin mediates apoptosis by interacting with Bcl-XL through C-terminal coiled coil domain.. Journal of cellular physiology, [PMID:21567405]
46. Li, N N, Zoubeidi, A A, Beraldi, E E and Gleave, M E ME.. (2013) GRP78 regulates clusterin stability, retrotranslocation and mitochondrial localization under ER stress in prostate cancer.. Oncogene, (11): [PMID:22689054]
47. Prochnow, Hans H and 5 more authors.. (2013) Non-secreted clusterin isoforms are translated in rare amounts from distinct human mRNA variants and do not affect Bax-mediated apoptosis or the NF-κB signaling pathway.. PloS one, [PMID:24073260]
48. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569]
49. Leeb, Christian C, Eresheim, Christine C and Nimpf, Johannes J.. (2014) Clusterin is a ligand for apolipoprotein E receptor 2 (ApoER2) and very low density lipoprotein receptor (VLDLR) and signals via the Reelin-signaling pathway.. The Journal of biological chemistry, (14): [PMID:24381170]
50. Rohne, Philipp P, Prochnow, Hans H, Wolf, Steven S, Renner, Benjamin B and Koch-Brandt, Claudia C.. (2014) The chaperone activity of clusterin is dependent on glycosylation and redox environment.. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, [PMID:25402950]

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Recombinant Clusterin Antibody - Primary antibody, specific to Clusterin, Rabbit IgG