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Ab096938-10μl

10μl

In stock

$66.90

Ab096938-50μl

50μl

In stock

$199.90

Ab096938-100μl

100μl

Available within 8-12 weeks(?)

$299.90

Ab096938-1ml

1ml

Available within 8-12 weeks(?)

$2,499.90

Basic Description

Product Name	Recombinant Clusterin Antibody - Primary antibody, specific to Clusterin, Rabbit IgG
Synonyms	40 antibody \| AAG 4 antibody \| AAG4 antibody \| Aging associated protein 4 antibody \| Aging-associated gene 4 protein antibody \| AI893575 antibody \| APO J antibody \| Apo-J antibody \| APOJ antibody \| ApoJalpha antibody \| ApoJbeta antibody \| Apolipoprotein J
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.1 mg/mL
Host species	Rabbit
Specificity	Clusterin
Immunogen	A synthetic peptide derived from human Clusterin (AA 400-449)
Positive Control	WB: Human serum, U-2 OS and HeLa lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human Clusterin Antibody, Recombinant, could be used for WB and so on. Application WB: 1/500-1/1000 Protein Function Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	0.1 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Clusterin Antibody (Ab096938) - Western Blot
All lanes: Recombinant Clusterin Antibody (Ab096938) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 37, 66 kDa
Exposure time: 32.1 s

Associated Targets(Human)

CLU Tbio Clusterin (0 Activities)

Discover Target: CLU

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000 "> 1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0505743	Certificate of Analysis	May 16, 2024	Ab096938
ZJ24F0505742	Certificate of Analysis	May 16, 2024	Ab096938

FAQs and Articles

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Protocol of Western Blotting For High Molecular Weights

Categories: Protocols

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

References

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2. Kirszbaum, L L, Bozas, S E SE and Walker, I D ID.. (1992) SP-40,40, a protein involved in the control of the complement pathway, possesses a unique array of disulphide bridges.. FEBS letters, (3): [PMID:1551440]
3. James, R W RW and 5 more authors.. () Characterization of a human high density lipoprotein-associated protein, NA1/NA2. Identity with SP-40,40, an inhibitor of complement-mediated cytolysis.. Arteriosclerosis and thrombosis : a journal of vascular biology, [PMID:1903064]
4. Danik, M M and 6 more authors.. (1991) Human gliomas and epileptic foci express high levels of a mRNA related to rat testicular sulfated glycoprotein 2, a purported marker of cell death.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1924317]
5. de Silva, H V HV, Harmony, J A JA, Stuart, W D WD, Gil, C M CM and Robbins, J J.. (1990) Apolipoprotein J: structure and tissue distribution.. Biochemistry, (5): [PMID:1974459]
6. de Silva, H V HV and 7 more authors.. (1990) Purification and characterization of apolipoprotein J.. The Journal of biological chemistry, (25): [PMID:2387851]
7. Choi, N H NH, Mazda, T T and Tomita, M M.. (1989) A serum protein SP40,40 modulates the formation of membrane attack complex of complement on erythrocytes.. Molecular immunology, [PMID:2601725]
8. Kirszbaum, L L and 6 more authors.. (1989) Molecular cloning and characterization of the novel, human complement-associated protein, SP-40,40: a link between the complement and reproductive systems.. The EMBO journal, [PMID:2721499]
9. Jenne, D E DE and Tschopp, J J.. (1989) Molecular structure and functional characterization of a human complement cytolysis inhibitor found in blood and seminal plasma: identity to sulfated glycoprotein 2, a constituent of rat testis fluid.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2780565]
10. Hochstrasser, A C AC and 6 more authors.. (1988) HDL particle associated proteins in plasma and cerebrospinal fluid: identification and partial sequencing.. Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society, [PMID:3154963]
11. Wong, P P and 5 more authors.. (1994) Molecular characterization of human TRPM-2/clusterin, a gene associated with sperm maturation, apoptosis and neurodegeneration.. European journal of biochemistry, (1): [PMID:8181474]
12. Kelso, G J GJ and 5 more authors.. (1994) Apolipoprotein J is associated with paraoxonase in human plasma.. Biochemistry, (25): [PMID:8292612]
13. Ghiso, J J and 6 more authors.. (1993) The cerebrospinal-fluid soluble form of Alzheimer's amyloid beta is complexed to SP-40,40 (apolipoprotein J), an inhibitor of the complement membrane-attack complex.. The Biochemical journal, (1): [PMID:8328966]
14. Reddy, K B KB, Karode, M C MC, Harmony, A K AK and Howe, P H PH.. (1996) Interaction of transforming growth factor beta receptors with apolipoprotein J/clusterin.. Biochemistry, (9): [PMID:8555189]
15. Kapron, J T JT and 6 more authors.. (1997) Identification and characterization of glycosylation sites in human serum clusterin.. Protein science : a publication of the Protein Society, [PMID:9336835]
16. Poon, S S, Easterbrook-Smith, S B SB, Rybchyn, M S MS, Carver, J A JA and Wilson, M R MR.. (2000) Clusterin is an ATP-independent chaperone with very broad substrate specificity that stabilizes stressed proteins in a folding-competent state.. Biochemistry, (26): [PMID:11123922]
17. Hatters, Danny M DM, Wilson, Mark R MR, Easterbrook-Smith, Simon B SB and Howlett, Geoffrey J GJ.. (2002) Suppression of apolipoprotein C-II amyloid formation by the extracellular chaperone, clusterin.. European journal of biochemistry, [PMID:12047389]
18. Poon, Stephen S and 5 more authors.. (2002) Mildly acidic pH activates the extracellular molecular chaperone clusterin.. The Journal of biological chemistry, (18): [PMID:12176985]
19. Leskov, Konstantin S KS, Klokov, Dmitry Y DY, Li, Jing J, Kinsella, Timothy J TJ and Boothman, David A DA.. (2003) Synthesis and functional analyses of nuclear clusterin, a cell death protein.. The Journal of biological chemistry, (28): [PMID:12551933]
20. Zhang, Hui H, Li, Xiao-Jun XJ, Martin, Daniel B DB and Aebersold, Ruedi R.. (2003) Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.. Nature biotechnology, [PMID:12754519]
21. Santilli, Giorgia G, Aronow, Bruce J BJ and Sala, Arturo A.. (2003) Essential requirement of apolipoprotein J (clusterin) signaling for IkappaB expression and regulation of NF-kappaB activity.. The Journal of biological chemistry, (3): [PMID:12882985]
22. Bunkenborg, Jakob J, Pilch, Bartosz J BJ, Podtelejnikov, Alexandre V AV and Wiśniewski, Jacek R JR.. (2004) Screening for N-glycosylated proteins by liquid chromatography mass spectrometry.. Proteomics, [PMID:14760718]
23. Takahashi, Meiko M, Lin, Yu-Min YM, Nakamura, Yusuke Y and Furukawa, Yoichi Y.. (2004) Isolation and characterization of a novel gene CLUAP1 whose expression is frequently upregulated in colon cancer.. Oncogene, (9): [PMID:15480429]
24. Zhang, Honglai H and 5 more authors.. (2005) Clusterin inhibits apoptosis by interacting with activated Bax.. Nature cell biology, [PMID:16113678]
25. Lewandrowski, Urs U, Moebius, Jan J, Walter, Ulrich U and Sickmann, Albert A.. (2006) Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach.. Molecular & cellular proteomics : MCP, [PMID:16263699]
26. Liu, Tao T and 6 more authors.. () Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952]
27. Nusbaum, Chad C and 75 more authors.. (2006) DNA sequence and analysis of human chromosome 8.. Nature, (19): [PMID:16421571]
28. Leong, Wai Fook WF and Chow, Vincent T K VT.. (2006) Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection.. Cellular microbiology, [PMID:16548883]
29. Ramachandran, Prasanna P and 5 more authors.. (2006) Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry.. Journal of proteome research, [PMID:16740002]
30. Trougakos, Ioannis P IP, Pawelec, Graham G, Tzavelas, Christos C, Ntouroupi, Triantaphillia T and Gonos, Efstathios S ES.. () Clusterin/Apolipoprotein J up-regulation after zinc exposure, replicative senescence or differentiation of human haematopoietic cells.. Biogerontology, [PMID:16955214]
31. Cochrane, Dawn R DR, Wang, Zhou Z, Muramaki, Motosugu M, Gleave, Martin E ME and Nelson, Colleen C CC.. (2007) Differential regulation of clusterin and its isoforms by androgens in prostate cells.. The Journal of biological chemistry, (26): [PMID:17148459]
32. Stewart, Elise M EM and 6 more authors.. (2007) Effects of glycosylation on the structure and function of the extracellular chaperone clusterin.. Biochemistry, (6): [PMID:17260971]
33. Andersen, Claus Lindbjerg CL and 7 more authors.. (2007) Clusterin expression in normal mucosa and colorectal cancer.. Molecular & cellular proteomics : MCP, [PMID:17322305]
34. Kumita, Janet R JR and 9 more authors.. (2007) The extracellular chaperone clusterin potently inhibits human lysozyme amyloid formation by interacting with prefibrillar species.. Journal of molecular biology, (25): [PMID:17407782]
35. Yerbury, Justin J JJ and 6 more authors.. (2007) The extracellular chaperone clusterin influences amyloid formation and toxicity by interacting with prefibrillar structures.. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, [PMID:17412999]
36. Nizard, Philippe P and 6 more authors.. (2007) Stress-induced retrotranslocation of clusterin/ApoJ into the cytosol.. Traffic (Copenhagen, Denmark), [PMID:17451556]
37. Wang, Zengjun Z, Widgren, Esther E EE, Richardson, Richard T RT and O'Rand, Michael G MG.. (2007) Characterization of an eppin protein complex from human semen and spermatozoa.. Biology of reproduction, [PMID:17567961]
38. Ranney, Melissa K MK, Ahmed, Ikhlas S A IS, Potts, Kelly R KR and Craven, Rolf J RJ.. (2007) Multiple pathways regulating the anti-apoptotic protein clusterin in breast cancer.. Biochimica et biophysica acta, [PMID:17689225]
39. Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31): [PMID:17974005]
40. Rizzi, Federica F, Caccamo, Alessandro Emanuele AE, Belloni, Lucia L and Bettuzzi, Saverio S.. (2009) Clusterin is a short half-life, poly-ubiquitinated protein, which controls the fate of prostate cancer cells.. Journal of cellular physiology, [PMID:19137541]
41. Wyatt, Amy R AR, Yerbury, Justin J JJ and Wilson, Mark R MR.. (2009) Structural characterization of clusterin-chaperone client protein complexes.. The Journal of biological chemistry, (14): [PMID:19535339]
42. Wyatt, Amy R AR and Wilson, Mark R MR.. (2010) Identification of human plasma proteins as major clients for the extracellular chaperone clusterin.. The Journal of biological chemistry, (5): [PMID:19996109]
43. Zoubeidi, Amina A and 8 more authors.. (2010) Clusterin facilitates COMMD1 and I-kappaB degradation to enhance NF-kappaB activity in prostate cancer cells.. Molecular cancer research : MCR, [PMID:20068069]
44. Wyatt, Amy R AR and 6 more authors.. (2011) Clusterin facilitates in vivo clearance of extracellular misfolded proteins.. Cellular and molecular life sciences : CMLS, [PMID:21505792]
45. Kim, Nayoung N and 13 more authors.. (2012) Human nuclear clusterin mediates apoptosis by interacting with Bcl-XL through C-terminal coiled coil domain.. Journal of cellular physiology, [PMID:21567405]
46. Li, N N, Zoubeidi, A A, Beraldi, E E and Gleave, M E ME.. (2013) GRP78 regulates clusterin stability, retrotranslocation and mitochondrial localization under ER stress in prostate cancer.. Oncogene, (11): [PMID:22689054]
47. Prochnow, Hans H and 5 more authors.. (2013) Non-secreted clusterin isoforms are translated in rare amounts from distinct human mRNA variants and do not affect Bax-mediated apoptosis or the NF-κB signaling pathway.. PloS one, [PMID:24073260]
48. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569]
49. Leeb, Christian C, Eresheim, Christine C and Nimpf, Johannes J.. (2014) Clusterin is a ligand for apolipoprotein E receptor 2 (ApoER2) and very low density lipoprotein receptor (VLDLR) and signals via the Reelin-signaling pathway.. The Journal of biological chemistry, (14): [PMID:24381170]
50. Rohne, Philipp P, Prochnow, Hans H, Wolf, Steven S, Renner, Benjamin B and Koch-Brandt, Claudia C.. (2014) The chaperone activity of clusterin is dependent on glycosylation and redox environment.. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, [PMID:25402950]

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Recombinant Clusterin Antibody - Primary antibody, specific to Clusterin, Rabbit IgG