| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab097188-10μl | 10μl | In stock | $66.90 | |
| Ab097188-50μl | 50μl | In stock | $189.90 | |
| Ab097188-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab097188-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human Collagen III Antibody; WB, ICC, IF; Unconjugated
| Product Name | Recombinant Collagen III Antibody - Primary antibody, specific to COL3A1, Rabbit IgG |
|---|---|
| Synonyms | Alpha 1 type III collagen antibody | Alpha1 (III) collagen antibody | CO3A1_HUMAN antibody | COL 3A1 antibody | COL3A1 antibody | Collagen alpha 1(III) chainantibody | Collagen alpha-1(III) chain antibody | Collagen III alpha 1 chain precursor antibody | |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.6 mg/mL |
| Host species | Rabbit |
| Specificity | COL3A1 |
| Immunogen | Recombinant protein of human Collagen III (AA 201-400) |
| Positive Control | WB: K562, HeLa, MCF7, A431, C6 and NIH/3T3 cell lysates. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Collagen III Antibody, Recombinant, could be used for WB, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Collagen III Antibody (Ab097188) - Western Blot
All lanes: Recombinant Collagen III Antibody (Ab097188) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 139 kDa
Observed band size: 152 kDa
Exposure time: 33.4 s
Recombinant Collagen III Antibody (Ab097188) - IF/ICC
Immunocytochemistry analysis of Collagen III (green) in HeLa using Recombinant Collagen III Antibody (Ab097188), and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
| WB |
1/500-1/1000
">
1/500-1/1000
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0102411 | Certificate of Analysis | Jan 10, 2024 | Ab097188 |
| ZJ24F0102412 | Certificate of Analysis | Jan 10, 2024 | Ab097188 |
| 1. Seyer, J M JM and Kang, A H AH.. (1977) Covalent structure of collagen: amino acid sequence of cyanogen bromide peptides from the amino-terminal segment of type III collagen of human liver.. Biochemistry, (22): [PMID:557335] |
| 2. Seyer, J M JM and Kang, A H AH.. (1978) Covalent structure of collagen: amino acid sequence of five consecutive CNBr peptides from type III collagen of human liver.. Biochemistry, (8): [PMID:687591] |
| 3. Richards, A J AJ and 5 more authors.. (1992) A single base mutation in the gene for type III collagen (COL3A1) converts glycine 847 to glutamic acid in a family with Ehlers-Danlos syndrome type IV. An unaffected family member is mosaic for the mutation.. Human genetics, [PMID:1352273] |
| 4. Johnson, P H PH, Richards, A J AJ, Pope, F M FM and Hopkinson, D A DA.. (1992) A COL3A1 glycine 1006 to glutamic acid substitution in a patient with Ehlers-Danlos syndrome type IV detected by denaturing gradient gel electrophoresis.. Journal of inherited metabolic disease, [PMID:1357232] |
| 5. Richards, A J AJ and 6 more authors.. (1992) A 27-bp deletion from one allele of the type III collagen gene (COL3A1) in a large family with Ehlers-Danlos syndrome type IV.. Human genetics, [PMID:1370809] |
| 6. Kontusaari, S S and 5 more authors.. (1992) Substitution of aspartate for glycine 1018 in the type III procollagen (COL3A1) gene causes type IV Ehlers-Danlos syndrome: the mutated allele is present in most blood leukocytes of the asymptomatic and mosaic mother.. American journal of human genetics, [PMID:1496983] |
| 7. Richards, A J AJ and 5 more authors.. (1991) Characterisation of a glycine to valine substitution at amino acid position 910 of the triple helical region of type III collagen in a patient with Ehlers-Danlos syndrome type IV.. Journal of medical genetics, [PMID:1895316] |
| 8. Cole, W G WG and 7 more authors.. (1990) A base substitution at a splice site in the COL3A1 gene causes exon skipping and generates abnormal type III procollagen in a patient with Ehlers-Danlos syndrome type IV.. The Journal of biological chemistry, (5): [PMID:2145268] |
| 9. Zafarullah, K K and 7 more authors.. (1990) G to A polymorphism in exon 31 of the COL3A1 gene.. Nucleic acids research, (25): [PMID:2235526] |
| 10. Kontusaari, S S, Tromp, G G, Kuivaniemi, H H, Romanic, A M AM and Prockop, D J DJ.. (1990) A mutation in the gene for type III procollagen (COL3A1) in a family with aortic aneurysms.. The Journal of clinical investigation, [PMID:2243125] |
| 11. Kontusaari, S S, Tromp, G G, Kuivaniemi, H H, Ladda, R L RL and Prockop, D J DJ.. (1990) Inheritance of an RNA splicing mutation (G+ 1 IVS20) in the type III procollagen gene (COL3A1) in a family having aortic aneurysms and easy bruisability: phenotypic overlap between familial arterial aneurysms and Ehlers-Danlos syndrome type IV.. American journal of human genetics, [PMID:2349939] |
| 12. Tromp, G G, Kuivaniemi, H H, Shikata, H H and Prockop, D J DJ.. (1989) A single base mutation that substitutes serine for glycine 790 of the alpha 1 (III) chain of type III procollagen exposes an arginine and causes Ehlers-Danlos syndrome IV.. The Journal of biological chemistry, (25): [PMID:2492273] |
| 13. Chu, M L ML and 5 more authors.. (1985) Isolation of cDNA and genomic clones encoding human pro-alpha 1 (III) collagen. Partial characterization of the 3' end region of the gene.. The Journal of biological chemistry, (10): [PMID:2579949] |
| 14. Ala-Kokko, L L, Kontusaari, S S, Baldwin, C T CT, Kuivaniemi, H H and Prockop, D J DJ.. (1989) Structure of cDNA clones coding for the entire prepro alpha 1 (III) chain of human type III procollagen. Differences in protein structure from type I procollagen and conservation of codon preferences.. The Biochemical journal, (1): [PMID:2764886] |
| 15. Benson-Chanda, V V, Su, M W MW, Weil, D D, Chu, M L ML and Ramirez, F F.. (1989) Cloning and analysis of the 5' portion of the human type-III procollagen gene (COL3A1).. Gene, (30): [PMID:2777083] |
| 16. Janeczko, R A RA and Ramirez, F F.. (1989) Nucleotide and amino acid sequences of the entire human alpha 1 (III) collagen.. Nucleic acids research, (25): [PMID:2780304] |
| 17. Tromp, G G, Kuivaniemi, H H, Stolle, C C, Pope, F M FM and Prockop, D J DJ.. (1989) Single base mutation in the type III procollagen gene that converts the codon for glycine 883 to aspartate in a mild variant of Ehlers-Danlos syndrome IV.. The Journal of biological chemistry, (15): [PMID:2808425] |
| 18. Molyneux, K K and Dalgleish, R R.. (1988) Human type III collagen 'variant' is a cDNA cloning artefact.. Nucleic acids research, (23): [PMID:3211760] |
| 19. Mankoo, B S BS and Dalgleish, R R.. (1988) Human pro alpha 1(III) collagen: cDNA sequence for the 3' end.. Nucleic acids research, (25): [PMID:3357782] |
| 20. Toman, P D PD, Ricca, G A GA and de Crombrugghe, B B.. (1988) Nucleotide sequence of a cDNA coding for the amino-terminal region of human prepro alpha 1(III) collagen.. Nucleic acids research, (25): [PMID:3405773] |
| 21. Miskulin, M M and 6 more authors.. (1986) Human type III collagen gene expression is coordinately modulated with the type I collagen genes during fibroblast growth.. Biochemistry, (25): [PMID:3754462] |
| 22. Emanuel, B S BS, Cannizzaro, L A LA, Seyer, J M JM and Myers, J C JC.. (1985) Human alpha 1(III) and alpha 2(V) procollagen genes are located on the long arm of chromosome 2.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3858826] |
| 23. Loidl, H R HR and 6 more authors.. (1984) Molecular cloning and carboxyl-propeptide analysis of human type III procollagen.. Nucleic acids research, (21): [PMID:6096827] |
| 24. Seyer, J M JM, Mainardi, C C and Kang, A H AH.. (1980) Covalent structure of collagen: amino acid sequence of alpha 1 (III)-CB5 from type III collagen of human liver.. Biochemistry, (15): [PMID:6246925] |
| 25. Seyer, J M JM and Kang, A H AH.. (1981) Covalent structure of collagen: amino acid sequence of alpha 1(III)-CB9 from type III collagen of human liver.. Biochemistry, (28): [PMID:7016180] |
| 26. Chiodo, A A AA, Sillence, D O DO, Cole, W G WG and Bateman, J F JF.. (1995) Abnormal type III collagen produced by an exon-17-skipping mutation of the COL3A1 gene in Ehlers-Danlos syndrome type IV is not incorporated into the extracellular matrix.. The Biochemical journal, (1): [PMID:7487954] |
| 27. Tromp, G G, De Paepe, A A, Nuytinck, L L, Madhatheri, S S and Kuivaniemi, H H.. (1995) Substitution of valine for glycine 793 in type III procollagen in Ehlers-Danlos syndrome type IV.. Human mutation, [PMID:7749417] |
| 28. Narcisi, P P, Richards, A J AJ, Ferguson, S D SD and Pope, F M FM.. (1994) A family with Ehlers-Danlos syndrome type III/articular hypermobility syndrome has a glycine 637 to serine substitution in type III collagen.. Human molecular genetics, [PMID:7833919] |
| 29. Madhatheri, S L SL, Tromp, G G, Gustavson, K H KH and Kuivaniemi, H H.. (1994) Substitution of glutamic acid for glycine 589 in the triple-helical domain of type III procollagen (COL3A1) in a family with variable phenotype of the Ehlers-Danlos syndrome type IV.. Human molecular genetics, [PMID:7912131] |
| 30. Nuytinck, L L, De Paepe, A A, Renard, J P JP, Adriaens, F F and Leroy, J J.. (1994) Single-strand conformation polymorphism (SSCP) analysis of the COL3A1 gene detects a mutation that results in the substitution of glycine 1009 to valine and causes severe Ehlers-Danlos syndrome type IV.. Human mutation, [PMID:8019562] |
| 31. Narcisi, P P and 6 more authors.. (1993) Single base mutation that substitutes glutamic acid for glycine 1021 in the COL3A1 gene and causes Ehlers-Danlos syndrome type IV.. American journal of medical genetics, (15): [PMID:8098182] |
| 32. Kuivaniemi, H H and 9 more authors.. (1993) Exclusion of mutations in the gene for type III collagen (COL3A1) as a common cause of intracranial aneurysms or cervical artery dissections: results from sequence analysis of the coding sequences of type III collagen from 55 unrelated patients.. Neurology, [PMID:8255472] |
| 33. Milewicz, D M DM and 5 more authors.. (1993) Parental somatic and germ-line mosaicism for a multiexon deletion with unusual endpoints in a type III collagen (COL3A1) allele produces Ehlers-Danlos syndrome type IV in the heterozygous offspring.. American journal of human genetics, [PMID:8317500] |
| 34. Richards, A A, Narcisi, P P, Lloyd, J J, Ferguson, C C and Pope, F M FM.. (1993) The substitution of glycine 661 by arginine in type III collagen produces mutant molecules with different thermal stabilities and causes Ehlers-Danlos syndrome type IV.. Journal of medical genetics, [PMID:8411057] |
| 35. Tromp, G G and 9 more authors.. (1993) Sequencing of cDNA from 50 unrelated patients reveals that mutations in the triple-helical domain of type III procollagen are an infrequent cause of aortic aneurysms.. The Journal of clinical investigation, [PMID:8514866] |
| 36. McGrory, J J, Costa, T T and Cole, W G WG.. (1996) A novel G499D substitution in the alpha 1(III) chain of type III collagen produces variable forms of Ehlers-Danlos syndrome type IV.. Human mutation, [PMID:8664902] |
| 37. Johnson, P H PH, Richards, A J AJ, Lloyd, J C JC, Pope, F M FM and Hopkinson, D A DA.. (1995) Efficient strategy for the detection of mutations in acrogeric Ehlers-Danlos syndrome type IV.. Human mutation, [PMID:8680408] |
| 38. Mackay, K K, Raghunath, M M, Superti-Furga, A A, Steinmann, B B and Dalgleish, R R.. (1996) Ehlers-Danlos syndrome type IV caused by Gly400Glu, Gly595Cys and Gly1003Asp substitutions in collagen III: clinical features, biochemical screening, and molecular confirmation.. Clinical genetics, [PMID:8884076] |
| 39. Anderson, D W DW, Thakker-Varia, S S, Tromp, G G, Kuivaniemi, H H and Stolle, C A CA.. (1997) A glycine (415)-to-serine substitution results in impaired secretion and decreased thermal stability of type III procollagen in a patient with Ehlers-Danlos syndrome type IV.. Human mutation, [PMID:8990011] |
| 40. Smith, L T LT, Schwarze, U U, Goldstein, J J and Byers, P H PH.. (1997) Mutations in the COL3A1 gene result in the Ehlers-Danlos syndrome type IV and alterations in the size and distribution of the major collagen fibrils of the dermis.. The Journal of investigative dermatology, [PMID:9036918] |
| 41. Kuivaniemi, H H, Tromp, G G and Prockop, D J DJ.. (1997) Mutations in fibrillar collagens (types I, II, III, and XI), fibril-associated collagen (type IX), and network-forming collagen (type X) cause a spectrum of diseases of bone, cartilage, and blood vessels.. Human mutation, [PMID:9101290] |
| 42. McGrory, J J, Weksberg, R R, Thorner, P P and Cole, W G WG.. (1996) Abnormal extracellular matrix in Ehlers-Danlos syndrome type IV due to the substitution of glycine 934 by glutamic acid in the triple helical domain of type III collagen.. Clinical genetics, [PMID:9147870] |
| 43. Pepin, M M, Schwarze, U U, Superti-Furga, A A and Byers, P H PH.. (2000) Clinical and genetic features of Ehlers-Danlos syndrome type IV, the vascular type.. The New England journal of medicine, (9): [PMID:10706896] |
| 44. Jansen, T T, de Paepe, A A, Luytinck, N N and Plewig, G G.. (2000) COL3A1 mutation leading to acrogeria (Gottron Type). The British journal of dermatology, [PMID:10819545] |
| 45. Giunta, C C and Steinmann, B B.. (2000) Characterization of 11 new mutations in COL3A1 of individuals with Ehlers-Danlos syndrome type IV: preliminary comparison of RNase cleavage, EMC and DHPLC assays.. Human mutation, [PMID:10923041] |
| 46. Nishiyama, Y Y and 11 more authors.. (2001) Ehlers-Danlos syndrome type IV with a unique point mutation in COL3A1 and familial phenotype of myocardial infarction without organic coronary stenosis.. Journal of internal medicine, [PMID:11168790] |
| 47. Välkkilä, M M and 5 more authors.. (2001) Genomic organization of the human COL3A1 and COL5A2 genes: COL5A2 has evolved differently than the other minor fibrillar collagen genes.. Matrix biology : journal of the International Society for Matrix Biology, [PMID:11566270] |
| 48. Kroes, H Y HY, Pals, G G and van Essen, A J AJ.. (2003) Ehlers-Danlos syndrome type IV: unusual congenital anomalies in a mother and son with a COL3A1 mutation and a normal collagen III protein profile.. Clinical genetics, [PMID:12694234] |
| 49. Palmeri, S S and 9 more authors.. (2003) Neurological presentation of Ehlers-Danlos syndrome type IV in a family with parental mosaicism.. Clinical genetics, [PMID:12786757] |
| 50. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 51. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974] |
| 52. Chan, Ting-Fung TF and 8 more authors.. (2008) Natural variation in four human collagen genes across an ethnically diverse population.. Genomics, [PMID:18272325] |
| 53. Boudko, Sergei P SP and 5 more authors.. (2008) Crystal structure of human type III collagen Gly991-Gly1032 cystine knot-containing peptide shows both 7/2 and 10/3 triple helical symmetries.. The Journal of biological chemistry, (21): [PMID:18805790] |
| 54. Plancke, Aurélie A and 5 more authors.. (2009) Homozygosity for a null allele of COL3A1 results in recessive Ehlers-Danlos syndrome.. European journal of human genetics : EJHG, [PMID:19455184] |
| 55. Rosenow, Anja A and 6 more authors.. (2012) Resveratrol-induced changes of the human adipocyte secretion profile.. Journal of proteome research, (7): [PMID:22905912] |
| 56. Bourhis, Jean-Marie and 8 more authors.. (2012) Structural basis of fibrillar collagen trimerization and related genetic disorders.. Nature structural & molecular biology, [PMID:23001006] |
| 57. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 58. Jørgensen, Agnete A and 7 more authors.. (2015) Vascular Ehlers-Danlos Syndrome in siblings with biallelic COL3A1 sequence variants and marked clinical variability in the extended family.. European journal of human genetics : EJHG, [PMID:25205403] |
| 59. Vandervore, Laura L and 14 more authors.. (2017) Bi-allelic variants in COL3A1 encoding the ligand to GPR56 are associated with cobblestone-like cortical malformation, white matter changes and cerebellar cysts.. Journal of medical genetics, [PMID:28258187] |
| 60. Horn, Denise D and 7 more authors.. (2017) Biallelic COL3A1 mutations result in a clinical spectrum of specific structural brain anomalies and connective tissue abnormalities.. American journal of medical genetics. Part A, [PMID:28742248] |
