Recombinant (RR684); Rabbit anti Human COX IV Antibody; WB, IHC, Flow, IF, ICC, IP; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab097410-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab097410-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $177.90 | |
| Ab097410-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $237.90 | |
| Ab097410-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,797.90 |
Recombinant (RR684); Rabbit anti Human COX IV Antibody; WB, IHC, Flow, IF, ICC, IP; Unconjugated
| Product Name | Recombinant COX IV Antibody - Primary antibody, specific to COX4I1, Rabbit IgG |
|---|---|
| Synonyms | AL024441 antibody; COX 4 antibody; COX IV 1 antibody; COX IV antibody; COX IV-1 antibody; Cox4 antibody; COX41_HUMAN antibody; Cox4a antibody; COX4B antibody; COX4I1 antibody; COX4I2 antibody; COX4L2 antibody; COXIV antibody; Cytochrome c oxidase polypept |
| Specifications & Purity | ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | COX4I1 |
| Immunogen | A synthetic peptide corresponding to residues on the N-terminus of human COX IV was used as an immunogen. |
| Positive Control | WB: A431, HEK293, HeLa, Jurkat, MCF7, A549, THP-1, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Liver tissue. Flow: HeLa cells. IF/ICC: HeLa cells. IP: HeLa whole cell lysate. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High performance, Recombinant, Validated |
| Product Description | Rabbit anti Human COX IV Antibody, Recombinant antibody (RR684), could be used for WB, IHC, Flow, IF, ICC, IP and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant COX IV Antibody (Ab097410) - Western Blot
All lanes: Recombinant COX IV Antibody (Ab097410) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 18 kDa
Exposure time: 15.1 s
Recombinant COX IV Antibody (Ab097410) - IF
IF analysis of COX IV (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant COX IV Antibody (Ab097410) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of liver tissue labelling COX IV with Recombinant COX IV Antibody (Ab097410) at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant COX IV Antibody (Ab097410) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant COX IV Antibody (Ab097410)(Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant COX IV Antibody (Ab097410) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
Recombinant COX IV Antibody (Ab097410) - IP
COX IV was immunoprecipitated from 0.4 mg of HeLa whole cell lysate with Recombinant COX IV Antibody (Ab097410) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant COX IV Antibody (Ab097410) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant COX IV Antibody (Ab097410) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input).
Exposure: 20.0 s
Recombinant COX IV Antibody (Ab097410) - IF
IF analysis of COX IV (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (TRITC) (Ab176448) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
Recombinant COX IV Antibody (Ab097410) - IF
IF analysis of COX IV (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ab176446) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IP |
1/50
|
| Flow |
1/200 - 1/1000
|
| WB |
1/5000 – 1/10000
|
| IF/ICC | 1/10 - 1/500 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0901104 | Certificate of Analysis | Sep 18, 2023 | Ab097410 |
| ZJ23F0901105 | Certificate of Analysis | Sep 18, 2023 | Ab097410 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Shuai Zong,Meng Wu,Jinke Gu,Tianya Liu,Runyu Guo,Maojun Yang. (2018-07-22) Structure of the intact 14-subunit human cytochrome c oxidase.. Cell research, 28 ((10)): (1026-1034). [PMID:30030519] |
| 3. Van Kuilenburg, A B AB and 5 more authors.. (1992) Subunit IV of human cytochrome c oxidase, polymorphism and a putative isoform.. Biochimica et biophysica acta, (26): [PMID:1311608] |
| 4. Lomax, M I MI, Welch, M D MD, Darras, B T BT, Francke, U U and Grossman, L I LI.. (1990) Novel use of a chimpanzee pseudogene for chromosomal mapping of human cytochrome c oxidase subunit IV.. Gene, (14): [PMID:2157630] |
| 5. Zeviani, M M and 8 more authors.. (1987) Isolation of a cDNA clone encoding subunit IV of human cytochrome c oxidase.. Gene, [PMID:2444497] |
| 6. Bachman, N J NJ, Wu, W W, Schmidt, T R TR, Grossman, L I LI and Lomax, M I MI.. (1999) The 5' region of the COX4 gene contains a novel overlapping gene, NOC4.. Mammalian genome : official journal of the International Mammalian Genome Society, [PMID:10337626] |
| 7. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 8. Cesnekova, Jana J and 5 more authors.. (2016) The mammalian homologue of yeast Afg1 ATPase (lactation elevated 1) mediates degradation of nuclear-encoded complex IV subunits.. The Biochemical journal, (15): [PMID:26759378] |
| 9. Abu-Libdeh, Bassam B and 7 more authors.. (2017) Mutation in the COX4I1 gene is associated with short stature, poor weight gain and increased chromosomal breaks, simulating Fanconi anemia.. European journal of human genetics : EJHG, [PMID:28766551] |
| 10. Pillai, Nishitha R NR and 9 more authors.. (2019) Biallelic variants in COX4I1 associated with a novel phenotype resembling Leigh syndrome with developmental regression, intellectual disability, and seizures.. American journal of medical genetics. Part A, (10): [PMID:31290619] |
