| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab098022-10μl | 10μl | In stock | $66.90 | |
| Ab098022-50μl | 50μl | In stock | $199.90 | |
| Ab098022-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab098022-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human CtBP1 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Recombinant CtBP1 Antibody - Primary antibody, specific to CTBP1, Rabbit IgG |
|---|---|
| Synonyms | BARS | brefeldin A-ribosylated substrate | CTBP | CtBP1 | C-terminal binding protein 1 | C-terminal-binding protein 1 | EC 1.1.1 | EC 1.1.1.- | EC 1.1.1.158 | MGC104684 | BARS antibody | brefeldin A- ribosylated substrate antibody | C terminal binding pro |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | CTBP1 |
| Immunogen | A synthetic peptide derived from human CtBP1 (AA 370-383). |
| Positive Control | WB: C6, NIH/3T3, Jurkat, Hep G2, HEK293, A549, MCF-7, Raji and HeLa cell lysates. IHC: Human tonsil tissue. ICC/IF: Hep G2 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human CtBP1 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant CtBP1 Antibody (Ab098022) - Western Blot
All lanes: Recombinant CtBP1 Antibody (Ab098022) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 43, 46 kDa
Exposure time: 33.1 s
Recombinant CtBP1 Antibody (Ab098022) - IF/ICC
Immunocytochemistry analysis of CTBP1 (green) in Hep G2 using Recombinant CtBP1 Antibody (Ab098022), and cells were counterstained with DAPI (blue).
Recombinant CtBP1 Antibody (Ab098022) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant CtBP1 Antibody (Ab098022). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0102511 | Certificate of Analysis | Jan 17, 2024 | Ab098022 |
| ZJ24F0102510 | Certificate of Analysis | Jan 17, 2024 | Ab098022 |
| 1. Vivek Kumar,Justin E Carlson,Kenneth A Ohgi,Thomas A Edwards,David W Rose,Carlos R Escalante,Michael G Rosenfeld,Aneel K Aggarwal. (2002-11-07) Transcription corepressor CtBP is an NAD(+)-regulated dehydrogenase.. Molecular cell, 10 ((4)): (857-869). [PMID:12419229] |
| 2. Schaeper, U U and 5 more authors.. (1995) Molecular cloning and characterization of a cellular phosphoprotein that interacts with a conserved C-terminal domain of adenovirus E1A involved in negative modulation of oncogenic transformation.. Proceedings of the National Academy of Sciences of the United States of America, (7): [PMID:7479821] |
| 3. Boyd, J M JM and 5 more authors.. (1993) A region in the C-terminus of adenovirus 2/5 E1a protein is required for association with a cellular phosphoprotein and important for the negative modulation of T24-ras mediated transformation, tumorigenesis and metastasis.. The EMBO journal, [PMID:8440238] |
| 4. Sewalt, R G RG, Gunster, M J MJ, van der Vlag, J J, Satijn, D P DP and Otte, A P AP.. (1999) C-Terminal binding protein is a transcriptional repressor that interacts with a specific class of vertebrate Polycomb proteins.. Molecular and cellular biology, [PMID:9858600] |
| 5. Touitou, R R, Hickabottom, M M, Parker, G G, Crook, T T and Allday, M J MJ.. (2001) Physical and functional interactions between the corepressor CtBP and the Epstein-Barr virus nuclear antigen EBNA3C.. Journal of virology, [PMID:11462050] |
| 6. Vo, N N, Fjeld, C C and Goodman, R H RH.. (2001) Acetylation of nuclear hormone receptor-interacting protein RIP140 regulates binding of the transcriptional corepressor CtBP.. Molecular and cellular biology, [PMID:11509661] |
| 7. Chakraborty, S S, Senyuk, V V, Sitailo, S S, Chi, Y Y and Nucifora, G G.. (2001) Interaction of EVI1 with cAMP-responsive element-binding protein-binding protein (CBP) and p300/CBP-associated factor (P/CAF) results in reversible acetylation of EVI1 and in co-localization in nuclear speckles.. The Journal of biological chemistry, (30): [PMID:11568182] |
| 8. Hickabottom, Mark M, Parker, Gillian A GA, Freemont, Paul P, Crook, Tim T and Allday, Martin J MJ.. (2002) Two nonconsensus sites in the Epstein-Barr virus oncoprotein EBNA3A cooperate to bind the co-repressor carboxyl-terminal-binding protein (CtBP).. The Journal of biological chemistry, (6): [PMID:12372828] |
| 9. Kagey, Michael H MH, Melhuish, Tiffany A TA and Wotton, David D.. (2003) The polycomb protein Pc2 is a SUMO E3.. Cell, (4): [PMID:12679040] |
| 10. Zhang, Qinghong Q, Yoshimatsu, Yasuhiro Y, Hildebrand, Jeffrey J, Frisch, Steven M SM and Goodman, Richard H RH.. (2003) Homeodomain interacting protein kinase 2 promotes apoptosis by downregulating the transcriptional corepressor CtBP.. Cell, (17): [PMID:14567915] |
| 11. Castet, Audrey A and 8 more authors.. (2004) Multiple domains of the Receptor-Interacting Protein 140 contribute to transcription inhibition.. Nucleic acids research, [PMID:15060175] |
| 12. Alpatov, Roman R and 7 more authors.. (2004) Nuclear speckle-associated protein Pnn/DRS binds to the transcriptional corepressor CtBP and relieves CtBP-mediated repression of the E-cadherin gene.. Molecular and cellular biology, [PMID:15542832] |
| 13. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 14. Nitta, Eriko E and 7 more authors.. (2005) Oligomerization of Evi-1 regulated by the PR domain contributes to recruitment of corepressor CtBP.. Oncogene, (8): [PMID:15897867] |
| 15. Long, Jianyin J, Zuo, Dongmei D and Park, Morag M.. (2005) Pc2-mediated sumoylation of Smad-interacting protein 1 attenuates transcriptional repression of E-cadherin.. The Journal of biological chemistry, (21): [PMID:16061479] |
| 16. Triantis, Vassilis V and 5 more authors.. (2006) Identification and characterization of DC-SCRIPT, a novel dendritic cell-expressed member of the zinc finger family of transcriptional regulators.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:16393996] |
| 17. Schön, Christian C, Wochnik, Angela A, Rössner, Antje A, Donow, Cornelia C and Knöchel, Walter W.. (2006) The FoxP subclass in Xenopus laevis development.. Development genes and evolution, [PMID:16609867] |
| 18. Ueda, Jun J, Tachibana, Makoto M, Ikura, Tsuyoshi T and Shinkai, Yoichi Y.. (2006) Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP.. The Journal of biological chemistry, (21): [PMID:16702210] |
| 19. Lopez-Garcia, Jorge J and 11 more authors.. (2006) ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases.. Nucleic acids research, [PMID:17085477] |
| 20. Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25): [PMID:17525332] |
| 21. Mendez, Lourdes M LM and 6 more authors.. (2008) CtBP is an essential corepressor for BCL6 autoregulation.. Molecular and cellular biology, [PMID:18212045] |
| 22. Purbey, Prabhat Kumar PK and 5 more authors.. (2009) Acetylation-dependent interaction of SATB1 and CtBP1 mediates transcriptional repression by SATB1.. Molecular and cellular biology, [PMID:19103759] |
| 23. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 24. Ono, Yasuko Y and 7 more authors.. (2013) PLEIAD/SIMC1/C5orf25, a novel autolysis regulator for a skeletal-muscle-specific calpain, CAPN3, scaffolds a CAPN3 substrate, CTBP1.. Journal of molecular biology, (23): [PMID:23707407] |
| 25. Subramanian, T T, Zhao, Ling-Jun LJ and Chinnadurai, G G.. (2013) Interaction of CtBP with adenovirus E1A suppresses immortalization of primary epithelial cells and enhances virus replication during productive infection.. Virology, (1): [PMID:23747199] |
| 26. Madison, Dana L DL, Wirz, Jacqueline A JA, Siess, Don D and Lundblad, James R JR.. (2013) Nicotinamide adenine dinucleotide-induced multimerization of the co-repressor CtBP1 relies on a switching tryptophan.. The Journal of biological chemistry, (27): [PMID:23940047] |
| 27. Hilbert, Brendan J and 6 more authors.. (2015) Structure-guided design of a high affinity inhibitor to human CtBP.. ACS chemical biology, (17): [PMID:25636004] |
| 28. Ichikawa, Kenji K and 6 more authors.. (2015) MCRIP1, an ERK substrate, mediates ERK-induced gene silencing during epithelial-mesenchymal transition by regulating the co-repressor CtBP.. Molecular cell, (2): [PMID:25728771] |
| 29. Beck, David B DB and 16 more authors.. (2016) A recurrent de novo CTBP1 mutation is associated with developmental delay, hypotonia, ataxia, and tooth enamel defects.. Neurogenetics, [PMID:27094857] |
| 30. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
