Recombinant; Rabbit anti Human CTBP2 Antibody; WB, IHC, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab098025-10μl | 10μl | In stock | $66.90 | |
| Ab098025-50μl | 50μl | In stock | $199.90 | |
| Ab098025-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab098025-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human CTBP2 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Recombinant CTBP2 Antibody - Primary antibody, specific to CTBP2, Rabbit IgG |
|---|---|
| Synonyms | C terminal binding protein 2; CtBP2; CTBP2_HUMAN; ribeye; C terminal binding protein 2 antibody; C-terminal-binding protein 2 antibody; CtBP2 antibody; CTBP2_HUMAN antibody; ribeye antibody |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.2 mg/mL |
| Host species | Rabbit |
| Specificity | CTBP2 |
| Immunogen | A synthetic peptide derived from human CTBP2 (AA 432-445). |
| Positive Control | WB: HeLa, A549, A431, MCF7, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human CTBP2 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant CTBP2 Antibody (Ab098025) - Western Blot
All lanes: Recombinant CTBP2 Antibody (Ab098025) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 46 kDa
Exposure time: 11.3 s
Recombinant CTBP2 Antibody (Ab098025) - IF/ICC
Immunocytochemistry analysis of CTBP2 (green) in HeLa using Recombinant CTBP2 Antibody (Ab098025), and cells were counterstained with DAPI (blue).
Recombinant CTBP2 Antibody (Ab098025) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant CTBP2 Antibody (Ab098025). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0102651 | Certificate of Analysis | Jan 30, 2024 | Ab098025 |
| ZJ24F0102650 | Certificate of Analysis | Jan 30, 2024 | Ab098025 |
| 1. Korwar S, Morris BL, Parikh HI, Coover RA, Doughty TW, Love IM, Hilbert BJ, Royer WE, Kellogg GE, Grossman SR, Ellis KC.. (2016) Design, synthesis, and biological evaluation of substrate-competitive inhibitors of C-terminal Binding Protein (CtBP).. Bioorg Med Chem, 24 (12): (2707-2715). [PMID:27156192] |
| 2. Schaeper, U U and 5 more authors.. (1995) Molecular cloning and characterization of a cellular phosphoprotein that interacts with a conserved C-terminal domain of adenovirus E1A involved in negative modulation of oncogenic transformation.. Proceedings of the National Academy of Sciences of the United States of America, (7): [PMID:7479821] |
| 3. Katsanis, N N and Fisher, E M EM.. (1998) A novel C-terminal binding protein (CTBP2) is closely related to CTBP1, an adenovirus E1A-binding protein, and maps to human chromosome 21q21.3.. Genomics, (15): [PMID:9479502] |
| 4. Schmitz, F F, Königstorfer, A A and Südhof, T C TC.. (2000) RIBEYE, a component of synaptic ribbons: a protein's journey through evolution provides insight into synaptic ribbon function.. Neuron, [PMID:11163272] |
| 5. Castet, Audrey A and 8 more authors.. (2004) Multiple domains of the Receptor-Interacting Protein 140 contribute to transcription inhibition.. Nucleic acids research, [PMID:15060175] |
| 6. Deloukas, P P and 135 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 10.. Nature, (27): [PMID:15164054] |
| 7. Alpatov, Roman R and 7 more authors.. (2004) Nuclear speckle-associated protein Pnn/DRS binds to the transcriptional corepressor CtBP and relieves CtBP-mediated repression of the E-cadherin gene.. Molecular and cellular biology, [PMID:15542832] |
| 8. Ueda, Jun J, Tachibana, Makoto M, Ikura, Tsuyoshi T and Shinkai, Yoichi Y.. (2006) Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP.. The Journal of biological chemistry, (21): [PMID:16702210] |
| 9. Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31): [PMID:17974005] |
| 10. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 11. Subramanian, T T, Zhao, Ling-Jun LJ and Chinnadurai, G G.. (2013) Interaction of CtBP with adenovirus E1A suppresses immortalization of primary epithelial cells and enhances virus replication during productive infection.. Virology, (1): [PMID:23747199] |
| 12. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315] |
| 13. Hilbert, Brendan J and 6 more authors.. (2015) Structure-guided design of a high affinity inhibitor to human CtBP.. ACS chemical biology, (17): [PMID:25636004] |
| 14. Ichikawa, Kenji K and 6 more authors.. (2015) MCRIP1, an ERK substrate, mediates ERK-induced gene silencing during epithelial-mesenchymal transition by regulating the co-repressor CtBP.. Molecular cell, (2): [PMID:25728771] |
