Recombinant (RR693); Rabbit anti Human Cytokeratin 18 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab098863-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab098863-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $207.90 | |
| Ab098863-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $297.90 | |
| Ab098863-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,517.90 |
Recombinant (RR693); Rabbit anti Human Cytokeratin 18 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated
| Product Name | Recombinant Cytokeratin 18 Antibody - Primary antibody, for WB,Flow,IHC,IP,IF/ICC various applications, from Human, generated in Rabbit, specific to KRT18, Rabbit IgG | |
|---|---|---|
| Synonyms | Cell proliferation inducing gene 46 protein antibody; Cell proliferation inducing protein 46antibody; Cell proliferation-inducing gene 46 protein antibody; CK 18 antibody; CK-18 antibody; CK18 antibody; CYK 18 antibody; CYK18 antibody; Cytokeratin 18 anti | |
| Specifications & Purity | ExactAb™, Validated, Recombinant, High performance, 0.5mg/mL | |
| Host Species | Rabbit | |
| Host species | Rabbit | |
| Specificity | KRT18 | |
| Application | WB,Flow,IHC,IP,IF/ICC | |
| Immunogen | A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 18 was used as an immunogen. | |
| Positive Control | WB: Hela, A431, HepG2, T47D, MCF-7, A549 cell lysate; Rat Liver. IHC: Colonic adenocarcinoma tissue. Flow: HCT-116 cells. ICC/IF: HCT-116 cells. IP: Hela whole cell lysate. | |
| Conjugation | Unconjugated | |
| Grade | ExactAb™, High Performance, Recombinant, Validated | |
| Product Description | Rabbit anti Human Cytokeratin 18 Antibody, Primary antibody, could be used for WB, IHC, Flow, IF/ICC, IP and so on. Application
Function Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. Post-translational Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. |
| Antibody Type | Primary antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | Rabbit IgG |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Source | CHO supernatant |
| Form | Liquid |
| Concentration | 0.5mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C for 2 years. Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Cytokeratin 18 Antibody (Ab098863) - Western Blot
All lanes: Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 44 kDa
Exposure time: 1.0 s
Recombinant Cytokeratin 18 Antibody (Ab098863) - ICC/IF
Recombinant Cytokeratin 18 Antibody (Ab098863) staining Cytokeratin 18 in HCT-116 cells by IF/ICC . Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1/2000) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor® 488- conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant Cytokeratin 18 Antibody (Ab098863) - IHC
Immunohistochemistry analysis of colonic adenocarcinoma tissue labelling Cytokeratin 18 with Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant Cytokeratin 18 Antibody (Ab098863) - Flow Cytometry
Overlay histogram showing HCT-116 cells stained Cytokeratin 18 with Recombinant Cytokeratin 18 Antibody (Ab098863) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (Ab098863, 1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
Recombinant Cytokeratin 18 Antibody (Ab098863) - IP
Cytokeratin 18 was immunoprecipitated from 0.4mg of HeLa whole cell lysate with Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/25 dilution.
Lane 1: Ab098863 IP in HeLa whole cell lysate
Lane 2: PBS instead of Ab098863 in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input)
Secondary: GAR HRP for IP 1:500
Exposure time: 20.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IF/ICC | 1/800 - 1/2000 |
| Flow Cytometry | 1/800 - 1/2000 |
| WB |
1/2000 - 1/5000
|
| IHC | 1/800 - 1/1600 |
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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0800786 | Certificate of Analysis | Aug 14, 2023 | Ab098863 |
| ZJ23F0800787 | Certificate of Analysis | Aug 14, 2023 | Ab098863 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Lixin Wang,Shanthi Srinivasan,Arianne L Theiss,Didier Merlin,Shanthi V Sitaraman. (2007-01-11) Interleukin-6 induces keratin expression in intestinal epithelial cells: potential role of keratin-8 in interleukin-6-induced barrier function alterations.. The Journal of biological chemistry, 282 ((11)): (8219-8227). [PMID:17213200] |
| 3. Francis Impens,Lilliana Radoshevich,Pascale Cossart,David Ribet. (2014-08-13) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.. Proceedings of the National Academy of Sciences of the United States of America, 111 ((34)): (12432-12437). [PMID:25114211] |
| 4. Romano, V V and 6 more authors.. (1986) Cytokeratin expression in simple epithelia. I. Identification of mRNA coding for human cytokeratin no. 18 by a cDNA clone.. Differentiation; research in biological diversity, [PMID:2422083] |
| 5. Oshima, R G RG, Millán, J L JL and Ceceña, G G.. (1986) Comparison of mouse and human keratin 18: a component of intermediate filaments expressed prior to implantation.. Differentiation; research in biological diversity, [PMID:2434380] |
| 6. Leube, R E RE and 5 more authors.. (1986) Cytokeratin expression in simple epithelia. III. Detection of mRNAs encoding human cytokeratins nos. 8 and 18 in normal and tumor cells by hybridization with cDNA sequences in vitro and in situ.. Differentiation; research in biological diversity, [PMID:2434381] |
| 7. Kulesh, D A DA and Oshima, R G RG.. (1988) Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells.. Molecular and cellular biology, [PMID:2454392] |
| 8. Ku, N O NO and Omary, M B MB.. (1994) Identification of the major physiologic phosphorylation site of human keratin 18: potential kinases and a role in filament reorganization.. The Journal of cell biology, [PMID:7523419] |
| 9. Ku, N O NO and Omary, M B MB.. (1995) Identification and mutational analysis of the glycosylation sites of human keratin 18.. The Journal of biological chemistry, (19): [PMID:7538124] |
| 10. Ku, N O NO, Michie, S S, Oshima, R G RG and Omary, M B MB.. (1995) Chronic hepatitis, hepatocyte fragility, and increased soluble phosphoglycokeratins in transgenic mice expressing a keratin 18 conserved arginine mutant.. The Journal of cell biology, [PMID:8522591] |
| 11. Ku, N O NO, Wright, T L TL, Terrault, N A NA, Gish, R R and Omary, M B MB.. (1997) Mutation of human keratin 18 in association with cryptogenic cirrhosis.. The Journal of clinical investigation, (1): [PMID:9011570] |
| 12. Ji, H H and 5 more authors.. () A two-dimensional gel database of human colon carcinoma proteins.. Electrophoresis, [PMID:9150948] |
| 13. Caulín, C C, Salvesen, G S GS and Oshima, R G RG.. (1997) Caspase cleavage of keratin 18 and reorganization of intermediate filaments during epithelial cell apoptosis.. The Journal of cell biology, (22): [PMID:9298992] |
| 14. Ku, N O NO, Liao, J J and Omary, M B MB.. (1998) Phosphorylation of human keratin 18 serine 33 regulates binding to 14-3-3 proteins.. The EMBO journal, (1): [PMID:9524113] |
| 15. Shi, J J and Sugrue, S P SP.. (2000) Dissection of protein linkage between keratins and pinin, a protein with dual location at desmosome-intermediate filament complex and in the nucleus.. The Journal of biological chemistry, (19): [PMID:10809736] |
| 16. Hofmann, I I and 5 more authors.. (2000) Interaction of plakophilins with desmoplakin and intermediate filament proteins: an in vitro analysis.. Journal of cell science, [PMID:10852826] |
| 17. Izawa, I I and 5 more authors.. (2000) Identification of Mrj, a DnaJ/Hsp40 family protein, as a keratin 8/18 filament regulatory protein.. The Journal of biological chemistry, (3): [PMID:10954706] |
| 18. Inada, H H and 7 more authors.. (2001) Keratin attenuates tumor necrosis factor-induced cytotoxicity through association with TRADD.. The Journal of cell biology, (29): [PMID:11684708] |
| 19. Ku, Nam-On NO and 8 more authors.. (2003) Keratin 8 and 18 mutations are risk factors for developing liver disease of multiple etiologies.. Proceedings of the National Academy of Sciences of the United States of America, (13): [PMID:12724528] |
| 20. Waseem, Ahmad A and 5 more authors.. (2004) Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability.. Biochemistry, (10): [PMID:14756564] |
| 21. Toivola, Diana M DM and 5 more authors.. (2004) Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease.. Hepatology (Baltimore, Md.), [PMID:15368451] |
| 22. Davezac, Noélie N and 9 more authors.. (2004) Global proteomic approach unmasks involvement of keratins 8 and 18 in the delivery of cystic fibrosis transmembrane conductance regulator (CFTR)/deltaF508-CFTR to the plasma membrane.. Proteomics, [PMID:15529338] |
| 23. Nishizawa, Miwako M and 7 more authors.. (2005) Identification of trichoplein, a novel keratin filament-binding protein.. Journal of cell science, (1): [PMID:15731013] |
| 24. Kang, Su-Min SM, Shin, Min-Jung MJ, Kim, Jung-Hee JH and Oh, Jong-Won JW.. (2005) Proteomic profiling of cellular proteins interacting with the hepatitis C virus core protein.. Proteomics, [PMID:15846844] |
| 25. Lipecka, Joanna J and 7 more authors.. (2006) Rescue of DeltaF508-CFTR (cystic fibrosis transmembrane conductance regulator) by curcumin: involvement of the keratin 18 network.. The Journal of pharmacology and experimental therapeutics, [PMID:16424149] |
| 26. Yu, Li-Rong LR and 5 more authors.. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.. Journal of proteome research, [PMID:17924679] |
| 27. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 28. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 29. Srikanth, Budnar B, Vaidya, Milind M MM and Kalraiya, Rajiv D RD.. (2010) O-GlcNAcylation determines the solubility, filament organization, and stability of keratins 8 and 18.. The Journal of biological chemistry, (29): [PMID:20729549] |
| 30. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 31. Ahmad, Yasmeen; Boisvert, Francois-Michel; Lundberg, Emma; Uhlen, Mathias and Lamond, Angus I.. (2012) Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization.. Molecular & cellular proteomics : MCP, [PMID:22002106] |
| 32. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 33. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 34. Kuga, Takahisa T and 9 more authors.. (2013) A novel mechanism of keratin cytoskeleton organization through casein kinase Iα and FAM83H in colorectal cancer.. Journal of cell science, (15): [PMID:23902688] |
| 35. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315] |
| 36. Bouameur, Jamal-Eddine JE and 5 more authors.. (2014) Interaction of plectin with keratins 5 and 14: dependence on several plectin domains and keratin quaternary structure.. The Journal of investigative dermatology, [PMID:24940650] |
| 37. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
