Recombinant (RR693); Rabbit anti Human Cytokeratin 18 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab098863-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab098863-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $207.90 | |
| Ab098863-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $297.90 | |
| Ab098863-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,517.90 |
Recombinant (RR693); Rabbit anti Human Cytokeratin 18 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated
| Product Name | Recombinant Cytokeratin 18 Antibody - Primary antibody, for WB,Flow,IHC,IP,IF/ICC various applications, from Human, generated in Rabbit, specific to KRT18, Rabbit IgG | |
|---|---|---|
| Synonyms | Cell proliferation inducing gene 46 protein antibody; Cell proliferation inducing protein 46antibody; Cell proliferation-inducing gene 46 protein antibody; CK 18 antibody; CK-18 antibody; CK18 antibody; CYK 18 antibody; CYK18 antibody; Cytokeratin 18 anti | |
| Specifications & Purity | ExactAb™, Validated, Recombinant, High performance, 0.5mg/mL | |
| Host Species | Rabbit | |
| Host species | Rabbit | |
| Specificity | KRT18 | |
| Application | WB,Flow,IHC,IP,IF/ICC | |
| Immunogen | A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 18 was used as an immunogen. | |
| Positive Control | WB: Hela, A431, HepG2, T47D, MCF-7, A549 cell lysate; Rat Liver. IHC: Colonic adenocarcinoma tissue. Flow: HCT-116 cells. ICC/IF: HCT-116 cells. IP: Hela whole cell lysate. | |
| Conjugation | Unconjugated | |
| Grade | ExactAb™, High performance, Recombinant, Validated | |
| Product Description | Rabbit anti Human Cytokeratin 18 Antibody, Primary antibody, could be used for WB, IHC, Flow, IF/ICC, IP and so on. Application
Function Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. Post-translational Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. |
| Antibody Type | Primary antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | Rabbit IgG |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Source | CHO supernatant |
| Form | Liquid |
| Concentration | 0.5mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C for 2 years. Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Cytokeratin 18 Antibody (Ab098863) - Western Blot
All lanes: Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 44 kDa
Exposure time: 1.0 s
Recombinant Cytokeratin 18 Antibody (Ab098863) - ICC/IF
Recombinant Cytokeratin 18 Antibody (Ab098863) staining Cytokeratin 18 in HCT-116 cells by IF/ICC . Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1/2000) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor® 488- conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant Cytokeratin 18 Antibody (Ab098863) - IHC
Immunohistochemistry analysis of colonic adenocarcinoma tissue labelling Cytokeratin 18 with Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant Cytokeratin 18 Antibody (Ab098863) - Flow Cytometry
Overlay histogram showing HCT-116 cells stained Cytokeratin 18 with Recombinant Cytokeratin 18 Antibody (Ab098863) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (Ab098863, 1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
Recombinant Cytokeratin 18 Antibody (Ab098863) - IP
Cytokeratin 18 was immunoprecipitated from 0.4mg of HeLa whole cell lysate with Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/25 dilution.
Lane 1: Ab098863 IP in HeLa whole cell lysate
Lane 2: PBS instead of Ab098863 in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input)
Secondary: GAR HRP for IP 1:500
Exposure time: 20.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IF/ICC | 1/800 - 1/2000 |
| Flow | 1/800 - 1/2000 |
| WB |
1/2000 - 1/5000
|
| IHC | 1/800 - 1/1600 |
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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0800786 | Certificate of Analysis | Aug 14, 2023 | Ab098863 |
| ZJ23F0800787 | Certificate of Analysis | Aug 14, 2023 | Ab098863 |
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