Grouped product items

SKU

Size

Availability

Price

Qty

Ab099347-10μl

10μl

Available within 8-12 weeks(?)

$79.90

Ab099347-50μl

50μl

Available within 8-12 weeks(?)

$219.90

Ab099347-100μl

100μl

Available within 8-12 weeks(?)

$359.90

Ab099347-1ml

1ml

Available within 8-12 weeks(?)

$2,699.90

Basic Description

Product Name	Recombinant DDB1 Antibody - Primary antibody, specific to DDB1, Rabbit IgG
Synonyms	Damage-specific DNA-binding protein 1 \| DDB p127 subunit \| DDBa \| DNA damage-binding protein a \| HBV X-associated protein 1 \| UV-damaged DNA-binding factor \| UV-damaged DNA-binding protein 1 \| UV-DDB 1 \| XAP-1 \| Xeroderma pigmentosum group E-complementing
Specifications & Purity	Recombinant, ExactAb™, Validated, KD Validation, High performance, Lot by Lot
Biochemical and Physiological Mechanisms	Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to init
Host species	Rabbit
Specificity	DDB1
Immunogen	A synthetic peptide derived from human DDB1 (AA 1100-1140)
Positive Control	WB: HeLa, MCF7, Hep G2, U-2 OS, Jurkat, NIH/3T3 and C6 cell lysates.
Conjugation	Unconjugated
Grade	ExactAb™, High Performance, KD Validation, Recombinant, Validated
Product Description	Rabbit anti Human DDB1 Antibody, Recombinant, could be used for WB and so on.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

DDB1 Tbio DNA damage-binding protein 1 (0 Activities)

Discover Target: DDB1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/1000-1/5000 "> 1/1000-1/5000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

References

1. Lopez-Girona A, Mendy D, Ito T, Miller K, Gandhi AK, Kang J, Karasawa S, Carmel G, Jackson P, Abbasian M et al.. (2012) Cereblon is a direct protein target for immunomodulatory and antiproliferative activities of lenalidomide and pomalidomide.. Leukemia, 26 (11): (2326-35). [PMID:22552008] [10.1021/op500134e]
2. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e]
3. Matyskiela ME, Zhang W, Man HW, Muller G, Khambatta G, Baculi F, Hickman M, LeBrun L, Pagarigan B, Carmel G et al.. (2018) A Cereblon Modulator (CC-220) with Improved Degradation of Ikaros and Aiolos.. J Med Chem, 61 (2): (535-542). [PMID:28425720] [10.1021/op500134e]
4. Chamberlain PP, Lopez-Girona A, Miller K, Carmel G, Pagarigan B, Chie-Leon B, Rychak E, Corral LG, Ren YJ, Wang M et al.. (2014) Structure of the human Cereblon-DDB1-lenalidomide complex reveals basis for responsiveness to thalidomide analogs.. Nat Struct Mol Biol, 21 (9): (803-9). [PMID:25108355] [10.1021/op500134e]
5. Fischer ES, Böhm K, Lydeard JR, Yang H, Stadler MB, Cavadini S, Nagel J, Serluca F, Acker V, Lingaraju GM et al.. (2014) Structure of the DDB1-CRBN E3 ubiquitin ligase in complex with thalidomide.. Nature, 512 (7512): (49-53). [PMID:25043012] [10.1021/op500134e]
6. Han T, Goralski M, Gaskill N, Capota E, Kim J, Ting TC, Xie Y, Williams NS, Nijhawan D. (2017) Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.. Science, 356 (6336): (1755-70). [PMID:28302793] [10.1021/op500134e]
7. Uehara T, Minoshima Y, Sagane K, Sugi NH, Mitsuhashi KO, Yamamoto N, Kamiyama H, Takahashi K, Kotake Y, Uesugi M et al.. (2017) Selective degradation of splicing factor CAPERα by anticancer sulfonamides.. Nat Chem Biol, 13 (6): (675-680). [PMID:28437394] [10.1021/op500134e]
8. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811]
9. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
10. Hansen JD, Correa M, Nagy MA, Alexander M, Plantevin V, Grant V, Whitefield B, Huang D, Kercher T, Harris R, Narla RK, Leisten J, Tang Y, Moghaddam M, Ebinger K, Piccotti J, Havens CG, Cathers B, Carmichael J, Daniel T, Vessey R, Hamann LG, Leftheris K, Mendy D, Baculi F, LeBrun LA, Khambatta G, Lopez-Girona A.. (2020) Discovery of CRBN E3 Ligase Modulator CC-92480 for the Treatment of Relapsed and Refractory Multiple Myeloma.. J Med Chem, 63 (13): (6648-6676). [PMID:32130004] [10.1021/acs.jmedchem.9b01928]
11. Lee, T H TH, Elledge, S J SJ and Butel, J S JS.. (1995) Hepatitis B virus X protein interacts with a probable cellular DNA repair protein.. Journal of virology, [PMID:7815490]
12. Dualan, R R and 5 more authors.. (1995) Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein.. Genomics, (1): [PMID:8530102]
13. Hwang, B J BJ, Liao, J C JC and Chu, G G.. (1996) Isolation of a cDNA encoding a UV-damaged DNA binding factor defective in xeroderma pigmentosum group E cells.. Mutation research, (2): [PMID:8538642]
14. Hwang, B J BJ, Toering, S S, Francke, U U and Chu, G G.. (1998) p48 Activates a UV-damaged-DNA binding factor and is defective in xeroderma pigmentosum group E cells that lack binding activity.. Molecular and cellular biology, [PMID:9632823]
15. Liu, W W and 5 more authors.. (2000) Nuclear transport of human DDB protein induced by ultraviolet light.. The Journal of biological chemistry, (14): [PMID:10777491]
16. Chen, X X, Zhang, Y Y, Douglas, L L and Zhou, P P.. (2001) UV-damaged DNA-binding proteins are targets of CUL-4A-mediated ubiquitination and degradation.. The Journal of biological chemistry, (21): [PMID:11673459]
17. Groisman, Regina R and 8 more authors.. (2003) The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage.. Cell, (2): [PMID:12732143]
18. Leupin, Olivier O, Bontron, Séverine S and Strubin, Michel M.. (2003) Hepatitis B virus X protein and simian virus 5 V protein exhibit similar UV-DDB1 binding properties to mediate distinct activities.. Journal of virology, [PMID:12743284]
19. Wertz, Ingrid E IE and 6 more authors.. (2004) Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin ligase.. Science (New York, N.Y.), (27): [PMID:14739464]
20. Hu, Jian J, McCall, Chad M CM, Ohta, Tomohiko T and Xiong, Yue Y.. (2004) Targeted ubiquitination of CDT1 by the DDB1-CUL4A-ROC1 ligase in response to DNA damage.. Nature cell biology, [PMID:15448697]
21. Sugasawa, Kaoru K and 10 more authors.. (2005) UV-induced ubiquitylation of XPC protein mediated by UV-DDB-ubiquitin ligase complex.. Cell, (6): [PMID:15882621]
22. Wittschieben, Birgitte Ø BØ, Iwai, Shigenori S and Wood, Richard D RD.. (2005) DDB1-DDB2 (xeroderma pigmentosum group E) protein complex recognizes a cyclobutane pyrimidine dimer, mismatches, apurinic/apyrimidinic sites, and compound lesions in DNA.. The Journal of biological chemistry, (2): [PMID:16223728]
23. Precious, B B, Childs, K K, Fitzpatrick-Swallow, V V, Goodbourn, S S and Randall, R E RE.. (2005) Simian virus 5 V protein acts as an adaptor, linking DDB1 to STAT2, to facilitate the ubiquitination of STAT1.. Journal of virology, [PMID:16227264]
24. Kulaksiz, Gülnihal G, Reardon, Joyce T JT and Sancar, Aziz A.. (2005) Xeroderma pigmentosum complementation group E protein (XPE/DDB2): purification of various complexes of XPE and analyses of their damaged DNA binding and putative DNA repair properties.. Molecular and cellular biology, [PMID:16260596]
25. Hu, Jian J and Xiong, Yue Y.. (2006) An evolutionarily conserved function of proliferating cell nuclear antigen for Cdt1 degradation by the Cul4-Ddb1 ubiquitin ligase in response to DNA damage.. The Journal of biological chemistry, (17): [PMID:16407242]
26. Senga, Takeshi T and 6 more authors.. (2006) PCNA is a cofactor for Cdt1 degradation by CUL4/DDB1-mediated N-terminal ubiquitination.. The Journal of biological chemistry, (10): [PMID:16407252]
27. Li, Ti T, Chen, Xiujuan X, Garbutt, Kenneth C KC, Zhou, Pengbo P and Zheng, Ning N.. (2006) Structure of DDB1 in complex with a paramyxovirus V protein: viral hijack of a propeller cluster in ubiquitin ligase.. Cell, (13): [PMID:16413485]
28. Kapetanaki, Maria G MG and 5 more authors.. (2006) The DDB1-CUL4ADDB2 ubiquitin ligase is deficient in xeroderma pigmentosum group E and targets histone H2A at UV-damaged DNA sites.. Proceedings of the National Academy of Sciences of the United States of America, (21): [PMID:16473935]
29. Nishitani, Hideo H and 11 more authors.. (2006) Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis.. The EMBO journal, (8): [PMID:16482215]
30. El-Mahdy, Mohamed A MA and 5 more authors.. (2006) Cullin 4A-mediated proteolysis of DDB2 protein at DNA damage sites regulates in vivo lesion recognition by XPC.. The Journal of biological chemistry, (12): [PMID:16527807]
31. Wang, Hengbin H and 8 more authors.. (2006) Histone H3 and H4 ubiquitylation by the CUL4-DDB-ROC1 ubiquitin ligase facilitates cellular response to DNA damage.. Molecular cell, (5): [PMID:16678110]
32. Lovejoy, Courtney A CA, Lock, Kimberli K, Yenamandra, Ashwini A and Cortez, David D.. (2006) DDB1 maintains genome integrity through regulation of Cdt1.. Molecular and cellular biology, [PMID:16940174]
33. Jin, Jianping J, Arias, Emily E EE, Chen, Jing J, Harper, J Wade JW and Walter, Johannes C JC.. (2006) A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1.. Molecular cell, (1): [PMID:16949367]
34. Angers, Stephane S and 5 more authors.. (2006) Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery.. Nature, (5): [PMID:16964240]
35. Higa, Leigh Ann LA and 5 more authors.. (2006) CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation.. Nature cell biology, [PMID:17041588]
36. He, Yizhou Joseph YJ, McCall, Chad M CM, Hu, Jian J, Zeng, Yaxue Y and Xiong, Yue Y.. (2006) DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4-ROC1 ubiquitin ligases.. Genes & development, (1): [PMID:17079684]
37. Höck, Julia J and 7 more authors.. (2007) Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.. EMBO reports, [PMID:17932509]
38. Huang, J J and Chen, J J.. (2008) VprBP targets Merlin to the Roc1-Cul4A-DDB1 E3 ligase complex for degradation.. Oncogene, (3): [PMID:18332868]
39. Hu, Jian J and 6 more authors.. (2008) WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase.. Genes & development, (1): [PMID:18381890]
40. Guerrero-Santoro, Jennifer J and 5 more authors.. (2008) The cullin 4B-based UV-damaged DNA-binding protein ligase binds to UV-damaged chromatin and ubiquitinates histone H2A.. Cancer research, (1): [PMID:18593899]
41. McCall, Chad M CM and 7 more authors.. (2008) Human immunodeficiency virus type 1 Vpr-binding protein VprBP, a WD40 protein associated with the DDB1-CUL4 E3 ubiquitin ligase, is essential for DNA replication and embryonic development.. Molecular and cellular biology, [PMID:18606781]
42. Scrima, Andrea A and 9 more authors.. (2008) Structural basis of UV DNA-damage recognition by the DDB1-DDB2 complex.. Cell, (26): [PMID:19109893]
43. Maddika, Subbareddy S and Chen, Junjie J.. (2009) Protein kinase DYRK2 is a scaffold that facilitates assembly of an E3 ligase.. Nature cell biology, [PMID:19287380]
44. Li, Ti T, Robert, Eva I EI, van Breugel, Pieter C PC, Strubin, Michel M and Zheng, Ning N.. (2010) A promiscuous alpha-helical motif anchors viral hijackers and substrate receptors to the CUL4-DDB1 ubiquitin ligase machinery.. Nature structural & molecular biology, [PMID:19966799]
45. Fischer, Eric S ES and 13 more authors.. (2011) The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation.. Cell, (23): [PMID:22118460]
46. Yeh, Joanne I JI and 10 more authors.. (2012) Damaged DNA induced UV-damaged DNA-binding protein (UV-DDB) dimerization and its roles in chromatinized DNA repair.. Proceedings of the National Academy of Sciences of the United States of America, (9): [PMID:22822215]
47. Shen, Zhen Z and Prasanth, Supriya G SG.. (2012) Orc2 protects ORCA from ubiquitin-mediated degradation.. Cell cycle (Georgetown, Tex.), (1): [PMID:22935713]
48. Abbas, Tarek T and 5 more authors.. (2013) CRL1-FBXO11 promotes Cdt2 ubiquitylation and degradation and regulates Pr-Set7/Set8-mediated cellular migration.. Molecular cell, (28): [PMID:23478445]
49. Tong, Xin X and 6 more authors.. (2015) CUL4-DDB1-CDT2 E3 Ligase Regulates the Molecular Clock Activity by Promoting Ubiquitination-Dependent Degradation of the Mammalian CRY1.. PloS one, [PMID:26431207]
50. Tong, Xin X and 8 more authors.. (2017) DDB1-Mediated CRY1 Degradation Promotes FOXO1-Driven Gluconeogenesis in Liver.. Diabetes, [PMID:28790135]
51. Mo, Yan Y and 6 more authors.. (2017) SIRT7 deacetylates DDB1 and suppresses the activity of the CRL4 E3 ligase complexes.. The FEBS journal, [PMID:28886238]
52. Shabek, Nitzan and 6 more authors.. (2018) Structural insights into DDA1 function as a core component of the CRL4-DDB1 ubiquitin ligase.. Cell discovery, [PMID:30564455]
53. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

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Recombinant DDB1 Antibody - Primary antibody, specific to DDB1, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Application

Certificates

Certificate of Analysis(COA)

Related Documents

References

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

SKU	Size	Availability	Price
Ab099347-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$79.90
Ab099347-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$219.90
Ab099347-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$359.90
Ab099347-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,699.90