Recombinant; Rabbit anti Human DDX6 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab099498-10μl | 10μl | In stock | $69.90 | |
| Ab099498-50μl | 50μl | In stock | $199.90 | |
| Ab099498-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab099498-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,499.90 |
Recombinant; Rabbit anti Human DDX6 Antibody; WB; Unconjugated
| Product Name | Recombinant DDX6 Antibody - Primary antibody, specific to DDX6, Rabbit IgG |
|---|---|
| Synonyms | ATP dependent RNA helicase DDX6 antibody | ATP dependent RNA helicase p54 antibody | DDX6 antibody | DEAD (Asp Glu Ala Asp) box polypeptide 6 antibody | DEAD box 6 antibody | DEAD box protein 6 antibody | DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RN |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.53 mg/mL |
| Host species | Rabbit |
| Specificity | DDX6 |
| Immunogen | A synthetic peptide derived from human DDX6 (AA 100-150) |
| Positive Control | WB: K562 lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human DDX6 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.53 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant DDX6 Antibody (Ab099498) - Western Blot
All lanes: Recombinant DDX6 Antibody (Ab099498) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 52.9 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0405002 | Certificate of Analysis | Apr 29, 2024 | Ab099498 |
| ZJ24F0405001 | Certificate of Analysis | Apr 29, 2024 | Ab099498 |
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| 8. Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25): [PMID:17525332] |
| 9. Tritschler, Felix F and 5 more authors.. (2009) Structural basis for the mutually exclusive anchoring of P body components EDC3 and Tral to the DEAD box protein DDX6/Me31B.. Molecular cell, (13): [PMID:19285948] |
| 10. James, Victoria V and 14 more authors.. (2010) LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing.. Proceedings of the National Academy of Sciences of the United States of America, (13): [PMID:20616046] |
| 11. Phalora, Prabhjeet K PK, Sherer, Nathan M NM, Wolinsky, Steven M SM, Swanson, Chad M CM and Malim, Michael H MH.. (2012) HIV-1 replication and APOBEC3 antiviral activity are not regulated by P bodies.. Journal of virology, [PMID:22915799] |
| 12. Loedige, Inga I, Gaidatzis, Dimos D, Sack, Ragna R, Meister, Gunter G and Filipowicz, Witold W.. (2013) The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function.. Nucleic acids research, (7): [PMID:23125361] |
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| 14. Ayache, Jessica J and 6 more authors.. (2015) P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes.. Molecular biology of the cell, (15): [PMID:25995375] |
| 15. Nishimura, Tamiko T and 6 more authors.. (2015) The eIF4E-Binding Protein 4E-T Is a Component of the mRNA Decay Machinery that Bridges the 5' and 3' Termini of Target mRNAs.. Cell reports, (9): [PMID:26027925] |
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| 17. Bish, Rebecca R and 6 more authors.. (2015) Comprehensive Protein Interactome Analysis of a Key RNA Helicase: Detection of Novel Stress Granule Proteins.. Biomolecules, (15): [PMID:26184334] |
| 18. Kamenska, Anastasiia A and 9 more authors.. (2016) The DDX6-4E-T interaction mediates translational repression and P-body assembly.. Nucleic acids research, (27): [PMID:27342281] |
| 19. Huang, Jo-Hsi JH, Ku, Wei-Chi WC, Chen, Yen-Chun YC, Chang, Yi-Ling YL and Chu, Chia-Ying CY.. (2017) Dual mechanisms regulate the nucleocytoplasmic localization of human DDX6.. Scientific reports, (20): [PMID:28216671] |
| 20. Balak, Chris and 37 more authors.. (2019) Rare De Novo Missense Variants in RNA Helicase DDX6 Cause Intellectual Disability and Dysmorphic Features and Lead to P-Body Defects and RNA Dysregulation.. American journal of human genetics, (5): [PMID:31422817] |
