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Recombinant DGCR8 Antibody - Primary antibody, specific to DGCR8, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 1.1 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human DGCR8 Antibody; WB, ICC, IF; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • ICC/IF
  • WB
Item Number
Ab099742
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab099742-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab099742-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$189.90
Ab099742-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab099742-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

Recombinant; Rabbit anti Human DGCR8 Antibody; WB, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant DGCR8 Antibody - Primary antibody, specific to DGCR8, Rabbit IgG
SynonymsDGCRK6 antibody; C22orf12 antibody; D16H22S788E antibody; D16Wis2 antibody; DGCR 8 antibody; Dgcr8 antibody; DGCR8 microprocessor complex subunit antibody; DGCR8_HUMAN antibody; DGCRK 6 antibody; DiGeorge syndrome critical region 8 antibody; DiGeorge synd
Specifications & PurityExactAb™, Validated, Recombinant, 1.1 mg/mL
Host speciesRabbit
SpecificityDGCR8
ImmunogenRecombinant protein of human DGCR8 (AA 424-773)
Positive ControlWB: K562, HeLa, HEK293, NIH/3T3 and C6 cell lysates. ICC/IF: K562 cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human DGCR8 Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function:
Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration1.1 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant DGCR8 Antibody (Ab099742) - Western Blot
All lanes: Recombinant DGCR8 Antibody (Ab099742) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 86 kDa
Observed band size: 103 kDa
Exposure time: 8.7 s

Recombinant DGCR8 Antibody (Ab099742) - Western Blot
All lanes: Recombinant DGCR8 Antibody (Ab099742) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 86 kDa
Observed band size: 103 kDa
Exposure time: 30.0 s

Recombinant DGCR8 Antibody (Ab099742) - IF/ICC
Immunocytochemistry analysis of DGCR8 (green) in K562 using Recombinant DGCR8 Antibody (Ab099742), and cells were counterstained with DAPI (blue).

Associated Targets

DGCR8 Tbio Microprocessor complex subunit DGCR8 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

ICC/IF

1/50-1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0606402Certificate of AnalysisJun 03, 2024 Ab099742
ZJ24F0606401Certificate of AnalysisJun 03, 2024 Ab099742

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