Recombinant DGCR8 Antibody - Primary antibody, specific to DGCR8, Rabbit IgG

Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human DGCR8 Antibody; WB, ICC, IF; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • IF/ICC
  • WB
Item Number
Ab099742
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab099742-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab099742-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$189.90
Ab099742-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab099742-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

Recombinant; Rabbit anti Human DGCR8 Antibody; WB, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant DGCR8 Antibody - Primary antibody, specific to DGCR8, Rabbit IgG
SynonymsDGCRK6 antibody | C22orf12 antibody | D16H22S788E antibody | D16Wis2 antibody | DGCR 8 antibody | Dgcr8 antibody | DGCR8 microprocessor complex subunit antibody | DGCR8_HUMAN antibody | DGCRK 6 antibody | DiGeorge syndrome critical region 8 antibody | Gy1
Specifications & PurityExactAb™, Validated, Recombinant, 1.1 mg/mL
Host speciesRabbit
SpecificityDGCR8
ImmunogenRecombinant protein of human DGCR8 (AA 424-773)
Positive ControlWB: K562, HeLa, HEK293, NIH/3T3 and C6 cell lysates. ICC/IF: K562 cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human DGCR8 Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function:
Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration1.1 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant DGCR8 Antibody (Ab099742) - Western Blot
All lanes: Recombinant DGCR8 Antibody (Ab099742) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 86 kDa
Observed band size: 103 kDa
Exposure time: 8.7 s

Recombinant DGCR8 Antibody (Ab099742) - Western Blot
All lanes: Recombinant DGCR8 Antibody (Ab099742) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 86 kDa
Observed band size: 103 kDa
Exposure time: 30.0 s

Recombinant DGCR8 Antibody (Ab099742) - IF/ICC
Immunocytochemistry analysis of DGCR8 (green) in K562 using Recombinant DGCR8 Antibody (Ab099742), and cells were counterstained with DAPI (blue).

Associated Targets(Human)

DGCR8 Tbio Microprocessor complex subunit DGCR8 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

ICC/IF

1/50-1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0606402Certificate of AnalysisJun 03, 2024 Ab099742
ZJ24F0606401Certificate of AnalysisJun 03, 2024 Ab099742

Related Documents

Solution Calculators