Recombinant DKC1/Dyskerin Antibody - Primary antibody, specific to DKC1, Rabbit IgG

Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human DKC1/Dyskerin Antibody; WB, IHC, IF, ICC; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • IF/ICC
  • IHC
  • WB
Item Number
Ab099931
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab099931-10μl
10μl
In stock
$66.90
Ab099931-50μl
50μl
In stock
$199.90
Ab099931-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab099931-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human DKC1/Dyskerin Antibody; WB, IHC, IF, ICC; Unconjugated

Basic Description

Product NameRecombinant DKC1/Dyskerin Antibody - Primary antibody, specific to DKC1, Rabbit IgG
SynonymsCBF5; CBF5 homolog; Cbf5p homolog; DKC 1; DKC; Dkc1; DKC1_HUMAN; DKCX; Dyskeratosis congenita 1; Dyskeratosis congenita 1 dyskerin; Dyskerin; H/ACA ribonucleoprotein complex subunit 4; NAP 57; NAP57; NAP-57; NOLA 4; NOLA4; Nopp140 associated protein of 57
Specifications & PurityExactAb™, Validated, Recombinant, 0.3 mg/mL
Host speciesRabbit
SpecificityDKC1
ImmunogenA synthetic peptide derived from human DKC1 (AA 371-384)
Positive ControlWB: HeLa, MCF7 and U2OS cell lysates. IHC: Human breast cancer tissue. ICC/IF: K562 cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human DKC1/Dyskerin Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells).

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.3 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant DKC1/Dyskerin Antibody (Ab099931) - Western Blot
All lanes: Recombinant DKC1/Dyskerin Antibody (Ab099931) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 45, 56 kDa
Exposure time: 79.4 s

Recombinant DKC1/Dyskerin Antibody (Ab099931) - IF/ICC
Immunocytochemistry analysis of DKC1 (green) in K562 using Recombinant DKC1/Dyskerin Antibody (Ab099931), and cells were counterstained with DAPI (blue).

Recombinant DKC1/Dyskerin Antibody (Ab099931) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant DKC1/Dyskerin Antibody (Ab099931). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

DKC1 Tbio H/ACA ribonucleoprotein complex subunit DKC1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

ICC/IF

1/50-1/200

IHC

1/50 - 1/100

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303270Certificate of AnalysisMar 14, 2024 Ab099931
ZJ24F0303269Certificate of AnalysisMar 14, 2024 Ab099931

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