A synthetic peptide derived from human DKC1 (AA 371-384)
Positive Control
WB: HeLa, MCF7 and U2OS cell lysates. IHC: Human breast cancer tissue. ICC/IF: K562 cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human DKC1/Dyskerin Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells).
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.3 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant DKC1/Dyskerin Antibody (Ab099931) - Western Blot All lanes: Recombinant DKC1/Dyskerin Antibody (Ab099931) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 58 kDa Observed band size: 45, 56 kDa Exposure time: 79.4 s
Recombinant DKC1/Dyskerin Antibody (Ab099931) - IF/ICC Immunocytochemistry analysis of DKC1 (green) in K562 using Recombinant DKC1/Dyskerin Antibody (Ab099931), and cells were counterstained with DAPI (blue).
Recombinant DKC1/Dyskerin Antibody (Ab099931) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant DKC1/Dyskerin Antibody (Ab099931). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.