Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they
Host species
Rabbit
Specificity
DKK1
Immunogen
A synthetic peptide derived from human DKK1 (AA 204-266).
Positive Control
WB: A431, Jurkat, HeLa lysates. IHC: Human placenta tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human DKK1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant DKK1 Antibody (Ab099940) - Western Blot All lanes: Recombinant DKK1 Antibody (Ab099940) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa Observed band size: 37, 41, 50 kDa Exposure time: 24.9 s
Recombinant DKK1 Antibody (Ab099940) - IHC Immunohistochemistry analysis of paraffin-embedded Human placenta using Recombinant DKK1 Antibody (Ab099940). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Associated Targets(Human)
DKK1TchemDickkopf-related protein 1 (0 Activities)