A synthetic peptide derived from human DKK1 (AA 204-266).
Positive Control
WB: HeLa, MOLT-4, HEK293 and MCF7 cell lysates. IHC: Human placenta tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human DKK1 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
1.2 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant DKK1 Antibody (Ab099940) - Western Blot All lanes: Recombinant DKK1 Antibody (Ab099940) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa Observed band size: 37, 41, 50 kDa Exposure time: 24.9 s
Recombinant DKK1 Antibody (Ab099940) - IHC Immunohistochemistry analysis of paraffin-embedded Human placenta using Recombinant DKK1 Antibody (Ab099940). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.