A synthetic peptide derived from human DOK1 (AA 430-481)
Positive Control
WB: K562, Jurkat, NIH/3T3 and C6 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human DOK1 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500 - 1/1000
Protein Function
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
Post-translational
Constitutively tyrosine-phosphorylated. Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant DOK1 Antibody (Ab100293) - Western Blot All lanes: Recombinant DOK1 Antibody (Ab100293) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 44, 58 kDa Exposure time: 21.5 s
Recombinant DOK1 Antibody (Ab100293) - Western Blot All lanes: Recombinant DOK1 Antibody (Ab100293) at 1/1000 dilution Samples: Lysates at 10 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 44, 58 kDa Exposure time: 3.0 s