| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab100461-10μl | 10μl | In stock | $66.90 | |
| Ab100461-50μl | 50μl | In stock | $189.90 | |
| Ab100461-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab100461-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human DR5 Antibody; WB; Unconjugated
| Product Name | Recombinant DR5 Antibody - Primary antibody, specific to TNFRSF10B, Rabbit IgG |
|---|---|
| Synonyms | Death receptor 5 | TNF-related apoptosis-inducing ligand receptor 2 | TRAIL receptor 2 | TRAIL-R2 |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.5 mg/mL |
| Host species | Rabbit |
| Specificity | TNFRSF10B |
| Immunogen | A synthetic peptide derived from human DR5 (AA 400-440) |
| Positive Control | WB: HT-29, Hep G2 and A549 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human DR5 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant DR5 Antibody (Ab100461) - Western Blot
All lanes: Recombinant DR5 Antibody (Ab100461) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 49, 52 kDa
Exposure time: 20.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 "> 1/500-1/2000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303767 | Certificate of Analysis | Mar 27, 2024 | Ab100461 |
| ZJ24F0303766 | Certificate of Analysis | Mar 27, 2024 | Ab100461 |
| 1. Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C et al.. (2003) The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.. Genome Res, 13 (10): (2265-70). [PMID:12975309] [10.1021/op500134e] |
| 2. Ohtsuki T, Kikuchi H, Koyano T, Kowithayakorn T, Sakai T, Ishibashi M.. (2009) Death receptor 5 promoter-enhancing compounds isolated from Catimbium speciosum and their enhancement effect on TRAIL-induced apoptosis.. Bioorg Med Chem, 17 (18): (6748-6754). [PMID:19682913] [10.1016/j.bmc.2009.07.041] |
| 3. Kikuchi H, Ohtsuki T, Koyano T, Kowithayakorn T, Sakai T, Ishibashi M.. (2010) Activity of mangosteen xanthones and teleocidin a-2 in death receptor expression enhancement and tumor necrosis factor related apoptosis-inducing ligand assays.. J Nat Prod, 73 (3): (452-455). [PMID:19788289] [10.1021/np900404e] |
| 4. Sheridan, J P JP and 12 more authors.. (1997) Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors.. Science (New York, N.Y.), (8): [PMID:9242611] |
| 5. Screaton, G R GR and 5 more authors.. (1997) TRICK2, a new alternatively spliced receptor that transduces the cytotoxic signal from TRAIL.. Current biology : CB, (1): [PMID:9285725] |
| 6. Walczak, H H and 11 more authors.. (1997) TRAIL-R2: a novel apoptosis-mediating receptor for TRAIL.. The EMBO journal, (1): [PMID:9311998] |
| 7. MacFarlane, M M and 5 more authors.. (1997) Identification and molecular cloning of two novel receptors for the cytotoxic ligand TRAIL.. The Journal of biological chemistry, (10): [PMID:9325248] |
| 8. Wu, G S GS and 14 more authors.. (1997) KILLER/DR5 is a DNA damage-inducible p53-regulated death receptor gene.. Nature genetics, [PMID:9326928] |
| 9. Schneider, P P and 5 more authors.. (1997) Characterization of two receptors for TRAIL.. FEBS letters, (27): [PMID:9373179] |
| 10. Chaudhary, P M PM and 5 more authors.. (1997) Death receptor 5, a new member of the TNFR family, and DR4 induce FADD-dependent apoptosis and activate the NF-kappaB pathway.. Immunity, [PMID:9430227] |
| 11. Arai, T T and 5 more authors.. (1998) Genomic organization and mutation analyses of the DR5/TRAIL receptor 2 gene in colorectal carcinomas.. Cancer letters, (27): [PMID:10072170] |
| 12. Mongkolsapaya, J J and 6 more authors.. (1999) Structure of the TRAIL-DR5 complex reveals mechanisms conferring specificity in apoptotic initiation.. Nature structural biology, [PMID:10542098] |
| 13. Hymowitz, S G SG and 7 more authors.. (1999) Triggering cell death: the crystal structure of Apo2L/TRAIL in a complex with death receptor 5.. Molecular cell, [PMID:10549288] |
| 14. Hillman, R Tyler RT, Green, Richard E RE and Brenner, Steven E SE.. (2004) An unappreciated role for RNA surveillance.. Genome biology, [PMID:14759258] |
| 15. Yamaguchi, Hirohito H and Wang, Hong-Gang HG.. (2004) CHOP is involved in endoplasmic reticulum stress-induced apoptosis by enhancing DR5 expression in human carcinoma cells.. The Journal of biological chemistry, (29): [PMID:15322075] |
| 16. Nusbaum, Chad C and 75 more authors.. (2006) DNA sequence and analysis of human chromosome 8.. Nature, (19): [PMID:16421571] |
| 17. Maddipatla, Sreeram S, Hernandez-Ilizaliturri, Francisco J FJ, Knight, Joy J and Czuczman, Myron S MS.. (2007) Augmented antitumor activity against B-cell lymphoma by a combination of monoclonal antibodies targeting TRAIL-R1 and CD20.. Clinical cancer research : an official journal of the American Association for Cancer Research, (1): [PMID:17671142] |
| 18. Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31): [PMID:17974005] |
| 19. Sun, M M, Song, L L, Li, Y Y, Zhou, T T and Jope, R S RS.. (2008) Identification of an antiapoptotic protein complex at death receptors.. Cell death and differentiation, [PMID:18846110] |
| 20. Smith, Wendell W and 17 more authors.. (2013) Human cytomegalovirus glycoprotein UL141 targets the TRAIL death receptors to thwart host innate antiviral defenses.. Cell host & microbe, (13): [PMID:23498957] |
| 21. Nemčovičová, Ivana; Benedict, Chris A and Zajonc, Dirk M.. (2013) Structure of human cytomegalovirus UL141 binding to TRAIL-R2 reveals novel, non-canonical death receptor interactions.. PLoS pathogens, [PMID:23555243] |
| 22. Newson, Joshua P M and 11 more authors.. (2019) Salmonella Effectors SseK1 and SseK3 Target Death Domain Proteins in the TNF and TRAIL Signaling Pathways.. Molecular & cellular proteomics : MCP, [PMID:30902834] |
| 23. Pan, G G and 5 more authors.. (1997) An antagonist decoy receptor and a death domain-containing receptor for TRAIL.. Science (New York, N.Y.), (8): [PMID:9242610] |
