Recombinant; Rabbit anti Human Drosha Antibody; WB; Unconjugate
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab100514-10μl | 10μl | In stock | $69.90 | |
| Ab100514-50μl | 50μl | In stock | $199.90 | |
| Ab100514-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab100514-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,499.90 |
Recombinant; Rabbit anti Human Drosha Antibody; WB; Unconjugate
| Product Name | Recombinant Drosha Antibody - Primary antibody, specific to Drosha, Rabbit IgG |
|---|---|
| Synonyms | DROSHA antibody | Drosha double stranded RNA specific endoribonuclease antibody | Drosha ribonuclease type III antibody | Etohi2 antibody | HSA242976 antibody | Nuclear RNase III Drosha antibody | p241 antibody | Protein Drosha antibody | Putative protein |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | Drosha |
| Immunogen | A synthetic peptide derived from human Drosha (AA 20-77) |
| Positive Control | WB: Jurkat and HeLa cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Drosha Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Drosha Antibody (Ab100514) - Western Blot
All lanes: Recombinant Drosha Antibody (Ab100514) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 159 kDa
Observed band size: 170 kDa
Exposure time: 114.2 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505504 | Certificate of Analysis | May 14, 2024 | Ab100514 |
| ZJ24F0505503 | Certificate of Analysis | May 14, 2024 | Ab100514 |
| 1. Wu, H H, Xu, H H, Miraglia, L J LJ and Crooke, S T ST.. (2000) Human RNase III is a 160-kDa protein involved in preribosomal RNA processing.. The Journal of biological chemistry, (24): [PMID:10948199] |
| 2. Gunther, M M, Laithier, M M and Brison, O O.. (2000) A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening.. Molecular and cellular biochemistry, [PMID:10976766] |
| 3. Lee, Yoontae Y and 10 more authors.. (2003) The nuclear RNase III Drosha initiates microRNA processing.. Nature, (25): [PMID:14508493] |
| 4. Schmutz, Jeremy J and 75 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 5.. Nature, (16): [PMID:15372022] |
| 5. Gregory, Richard I RI and 6 more authors.. (2004) The Microprocessor complex mediates the genesis of microRNAs.. Nature, (11): [PMID:15531877] |
| 6. Zeng, Yan Y, Yi, Rui R and Cullen, Bryan R BR.. (2005) Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha.. The EMBO journal, (12): [PMID:15565168] |
| 7. Han, Jinju J and 5 more authors.. (2004) The Drosha-DGCR8 complex in primary microRNA processing.. Genes & development, (15): [PMID:15574589] |
| 8. Landthaler, Markus M, Yalcin, Abdullah A and Tuschl, Thomas T.. (2004) The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis.. Current biology : CB, (14): [PMID:15589161] |
| 9. Han, Jinju J and 9 more authors.. (2006) Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex.. Cell, (2): [PMID:16751099] |
| 10. Pauley, Kaleb M KM and 5 more authors.. (2006) Formation of GW bodies is a consequence of microRNA genesis.. EMBO reports, [PMID:16906129] |
| 11. Faller, Michael M, Matsunaga, Michio M, Yin, Sheng S, Loo, Joseph A JA and Guo, Feng F.. (2007) Heme is involved in microRNA processing.. Nature structural & molecular biology, [PMID:17159994] |
| 12. Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31): [PMID:17974005] |
| 13. Yu, Bin B and 10 more authors.. (2008) The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis.. Proceedings of the National Academy of Sciences of the United States of America, (22): [PMID:18632581] |
| 14. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 15. Piskounova, Elena E and 7 more authors.. (2011) Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms.. Cell, (23): [PMID:22118463] |
| 16. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 17. Nguyen, Tuan Anh TA and 7 more authors.. (2015) Functional Anatomy of the Human Microprocessor.. Cell, (4): [PMID:26027739] |
| 18. Kwon, S Chul SC and 6 more authors.. (2016) Structure of Human DROSHA.. Cell, (14): [PMID:26748718] |
