Skip to the beginning of the images gallery

Recombinant Drosha Antibody - Primary antibody, specific to Drosha, Rabbit IgG

Recombinant

Validated

ExactAb™

1.0 mg/mL

Features and benefits

Short Overview:
Recombinant; Rabbit anti Human Drosha Antibody; WB; Unconjugate
Species reactivity(Reacts with): Human
Isotype: Rabbit IgG

Application:

Item Number

Ab100514

Grouped product items
SKU	Size	Availability	Price
Ab100514-10μl	10μl	In stock	$69.90
Ab100514-50μl	50μl	In stock	$199.90
Ab100514-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab100514-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,499.90

Recombinant; Rabbit anti Human Drosha Antibody; WB; Unconjugate

View related series

Click to view target information: DROSHA

Accession#:Q9NRR4 DROSHA Gene ID:29102

Basic Description

Product Name	Recombinant Drosha Antibody - Primary antibody, specific to Drosha, Rabbit IgG
Synonyms	DROSHA antibody \| Drosha double stranded RNA specific endoribonuclease antibody \| Drosha ribonuclease type III antibody \| Etohi2 antibody \| HSA242976 antibody \| Nuclear RNase III Drosha antibody \| p241 antibody \| Protein Drosha antibody \| Putative protein
Specifications & Purity	ExactAb™, Validated, Recombinant, 1.0 mg/mL
Host species	Rabbit
Specificity	Drosha
Immunogen	A synthetic peptide derived from human Drosha (AA 20-77)
Positive Control	WB: Jurkat and HeLa cell lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human Drosha Antibody, Recombinant, could be used for WB and so on. Application WB: 1/500-1/1000 Protein Function Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Drosha Antibody (Ab100514) - Western Blot
All lanes: Recombinant Drosha Antibody (Ab100514) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 159 kDa
Observed band size: 170 kDa
Exposure time: 114.2 s

Application

Dilution info

1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0505504	Certificate of Analysis	May 14, 2024	Ab100514
ZJ24F0505503	Certificate of Analysis	May 14, 2024	Ab100514

References

1. Wu, H H, Xu, H H, Miraglia, L J LJ and Crooke, S T ST.. (2000) Human RNase III is a 160-kDa protein involved in preribosomal RNA processing.. The Journal of biological chemistry, (24): [PMID:10948199]
2. Gunther, M M, Laithier, M M and Brison, O O.. (2000) A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening.. Molecular and cellular biochemistry, [PMID:10976766]
3. Lee, Yoontae Y and 10 more authors.. (2003) The nuclear RNase III Drosha initiates microRNA processing.. Nature, (25): [PMID:14508493]
4. Schmutz, Jeremy J and 75 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 5.. Nature, (16): [PMID:15372022]
5. Gregory, Richard I RI and 6 more authors.. (2004) The Microprocessor complex mediates the genesis of microRNAs.. Nature, (11): [PMID:15531877]
6. Zeng, Yan Y, Yi, Rui R and Cullen, Bryan R BR.. (2005) Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha.. The EMBO journal, (12): [PMID:15565168]
7. Han, Jinju J and 5 more authors.. (2004) The Drosha-DGCR8 complex in primary microRNA processing.. Genes & development, (15): [PMID:15574589]
8. Landthaler, Markus M, Yalcin, Abdullah A and Tuschl, Thomas T.. (2004) The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis.. Current biology : CB, (14): [PMID:15589161]
9. Han, Jinju J and 9 more authors.. (2006) Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex.. Cell, (2): [PMID:16751099]
10. Pauley, Kaleb M KM and 5 more authors.. (2006) Formation of GW bodies is a consequence of microRNA genesis.. EMBO reports, [PMID:16906129]
11. Faller, Michael M, Matsunaga, Michio M, Yin, Sheng S, Loo, Joseph A JA and Guo, Feng F.. (2007) Heme is involved in microRNA processing.. Nature structural & molecular biology, [PMID:17159994]
12. Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31): [PMID:17974005]
13. Yu, Bin B and 10 more authors.. (2008) The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis.. Proceedings of the National Academy of Sciences of the United States of America, (22): [PMID:18632581]
14. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
15. Piskounova, Elena E and 7 more authors.. (2011) Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms.. Cell, (23): [PMID:22118463]
16. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
17. Nguyen, Tuan Anh TA and 7 more authors.. (2015) Functional Anatomy of the Human Microprocessor.. Cell, (4): [PMID:26027739]
18. Kwon, S Chul SC and 6 more authors.. (2016) Structure of Human DROSHA.. Cell, (14): [PMID:26748718]
19. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Dilution Calculator

Determine the dilution needed to prepare a stock solution.