Basic Description

Product Name	Recombinant Eg5 Antibody - Primary antibody, specific to Eg5, Rabbit IgG
Synonyms	EG5 \| HKSP \| KNSL1 \| MCLMR \| TRIP5 \| EG5 antibody \| HKSP antibody \| KIF11 antibody \| KIF11_HUMAN antibody \| Kinesin family member 11 antibody \| Kinesin like protein 1 antibody \| Kinesin-like protein 1 antibody \| Kinesin-like protein KIF11 antibody \| Kines
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.6 mg/mL
Host species	Rabbit
Specificity	Eg5
Immunogen	Recombinant protein of human Eg5 (AA 8-399)
Positive Control	WB: HeLa, A549, U2OS, K562 and A431 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human Eg5 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function： Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	0.6 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Eg5 Antibody (Ab101062) - Western Blot
All lanes: Recombinant Eg5 Antibody (Ab101062) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 119 kDa
Observed band size: 121 kDa
Exposure time: 5.9 s

Recombinant Eg5 Antibody (Ab101062) - IF/ICC
Immunocytochemistry analysis of Eg5 (green) in HeLa using Recombinant Eg5 Antibody (Ab101062), and cells were counterstained with DAPI (blue).

Recombinant Eg5 Antibody (Ab101062) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Eg5 Antibody (Ab101062). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

KIF11 Tchem Kinesin-like protein KIF11 (0 Activities)

Discover Target: KIF11

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Application

Application	Dilution info
WB	1/500-1/1000 "> 1/500-1/1000
IF/ICC	1/50-1/200 "> 1/50-1/200
IHC	1/50 - 1/100 "> 1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

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2 results found

Lot Number	Chart	Certificate Type	Date	Item
ZJ24F0404427		Certificate of Analysis	Apr 11, 2024	Ab101062
ZJ24F0404426		Certificate of Analysis	Apr 11, 2024	Ab101062

FAQs and Articles

References

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4. Kim KS, Lu S, Cornelius LA, Lombardo LJ, Borzilleri RM, Schroeder GM, Sheng C, Rovnyak G, Crews D, Schmidt RJ, Williams DK, Bhide RS, Traeger SC, McDonnell PA, Mueller L, Sheriff S, Newitt JA, Pudzianowski AT, Yang Z, Wild R, Lee FY, Batorsky R, Ryder JS, Ortega-Nanos M, Shen H, Gottardis M, Roussell DL.. (2006) Synthesis and SAR of pyrrolotriazine-4-one based Eg5 inhibitors.. Bioorg Med Chem Lett, 16 (15): (3937-3942). [PMID:16730979] [10.1016/j.bmcl.2006.05.037]
5. Garcia-Saez I, DeBonis S, Lopez R, Trucco F, Rousseau B, Thuéry P, Kozielski F.. (2007) Structure of human Eg5 in complex with a new monastrol-based inhibitor bound in the R configuration.. J Biol Chem, 282 (13): (9740-9747). [PMID:17251189] [10.1074/jbc.m608883200]
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7. Rodriguez D, Ramesh C, Henson LH, Wilmeth L, Bryant BK, Kadavakollu S, Hirsch R, Montoya J, Howell PR, George JM, Alexander D, Johnson DL, Arterburn JB, Shuster CB.. (2011) Synthesis and characterization of tritylthioethanamine derivatives with potent KSP inhibitory activity.. Bioorg Med Chem, 19 (18): (5446-5453). [PMID:21855351] [10.1016/j.bmc.2011.07.054]
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9. Dong JJ, Li QS, Liu ZP, Wang SF, Zhao MY, Yang YH, Wang XM, Zhu HL.. (2013) Synthesis, biological evaluation and molecular docking studies of flavone and isoflavone derivatives as a novel class of KSP (kinesin spindle protein) inhibitors.. Eur J Med Chem, 70 (427-433). [PMID:24184776] [10.1016/j.ejmech.2013.09.042]
10. Yamamoto J, Amishiro N, Kato K, Ohta Y, Ino Y, Araki M, Tsujita T, Okamoto S, Takahashi T, Kusaka H, Akinaga S, Yamashita Y, Nakai R, Murakata C.. (2014) Synthetic studies on mitotic kinesin Eg5 inhibitors: synthesis and structure-activity relationships of novel 2,4,5-substituted-1,3,4-thiadiazoline derivatives.. Bioorg Med Chem Lett, 24 (16): (3961-3963). [PMID:25001485] [10.1016/j.bmcl.2014.06.034]
11. De Monte C, Carradori S, Secci D, D'Ascenzio M, Guglielmi P, Mollica A, Morrone S, Scarpa S, Aglianò AM, Giantulli S, Silvestri I.. (2015) Synthesis and pharmacological screening of a large library of 1,3,4-thiadiazolines as innovative therapeutic tools for the treatment of prostate cancer and melanoma.. Eur J Med Chem, 105 (245-262). [PMID:26498571] [10.1016/j.ejmech.2015.10.023]
12. Talapatra SK, Rath O, Clayton E, Tomasi S, Kozielski F.. (2016) Depsidones from Lichens as Natural Product Inhibitors of M-Phase Phosphoprotein 1, a Human Kinesin Required for Cytokinesis.. J Nat Prod, 79 (6): (1576-1585). [PMID:27300079] [10.1021/acs.jnatprod.5b00962]
13. Talapatra SK, Tham CL, Guglielmi P, Cirilli R, Chandrasekaran B, Karpoormath R, Carradori S, Kozielski F.. (2018) Crystal structure of the Eg5 - K858 complex and implications for structure-based design of thiadiazole-containing inhibitors.. Eur J Med Chem, 156 (641-651). [PMID:30031975] [10.1016/j.ejmech.2018.07.006]
14. Dumas ME, Chen GY, Kendrick ND, Xu G, Larsen SD, Jana S, Waterson AG, Bauer JA, Hancock W, Sulikowski GA, Ohi R.. (2019) Dual inhibition of Kif15 by oxindole and quinazolinedione chemical probes.. Bioorg Med Chem Lett, 29 (2): (148-154). [PMID:30528696] [10.1016/j.bmcl.2018.12.008]
15. Tawfik HO, El-Moselhy TF, El-Din NS, El-Hamamsy MH.. (2019) Design, synthesis, and bioactivity of dihydropyrimidine derivatives as kinesin spindle protein inhibitors.. Bioorg Med Chem, 27 (23): (115126-115126). [PMID:31648875] [10.1016/j.bmc.2019.115126]
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19. Turner, J J and 5 more authors.. (2001) Crystal structure of the mitotic spindle kinesin Eg5 reveals a novel conformation of the neck-linker.. The Journal of biological chemistry, (6): [PMID:11328809]
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22. Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25): [PMID:17525332]
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24. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648]
25. Rapley, Joseph J and 7 more authors.. (2008) The NIMA-family kinase Nek6 phosphorylates the kinesin Eg5 at a novel site necessary for mitotic spindle formation.. Journal of cell science, (1): [PMID:19001501]
26. Sahab, Ziad J ZJ and 6 more authors.. (2011) Tumor suppressor RARRES1 interacts with cytoplasmic carboxypeptidase AGBL2 to regulate the α-tubulin tyrosination cycle.. Cancer research, (15): [PMID:21303978]
27. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
28. Ostergaard, Pia P and 25 more authors.. (2012) Mutations in KIF11 cause autosomal-dominant microcephaly variably associated with congenital lymphedema and chorioretinopathy.. American journal of human genetics, (10): [PMID:22284827]
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30. Wakana, Yuichi Y and 5 more authors.. (2013) Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface.. The Journal of cell biology, (22): [PMID:23857769]
31. Zhang, Wei W and 5 more authors.. (2016) Discovery of Novel Allosteric Eg5 Inhibitors Through Structure-Based Virtual Screening.. Chemical biology & drug design, [PMID:26864917]
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Solution Calculators

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Dilution Calculator

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SKU	Size	Availability	Price
Ab101062-10μl	10μl	In stock	$69.90
Ab101062-50μl	50μl	In stock	$199.90
Ab101062-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$279.90
Ab101062-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,599.90

Recombinant Eg5 Antibody - Primary antibody, specific to Eg5, Rabbit IgG