Rabbit anti Human eIF2A Antibody, Recombinant, could be used for WB and ICC/IF. Application: WB: 1/500 – 1/1000 ICC/IF: 1/50 – 1/200 Protein Function Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant eIF2A Antibody (Ab101265) - Western Blot All lanes: Recombinant eIF2A Antibody (Ab101265) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 65 kDa Observed band size: 65&55 kDa Exposure time: 1.7 s
Recombinant eIF2A Antibody (Ab101265) - IF Immunocytochemistry analysis of eIF2A (green) in MCF7 using Recombinant eIF2A Antibody (Ab101265) and cells were counterstained with DAPI (blue).