Recombinant eIF2A Antibody - Primary antibody, specific to EIF2A, Rabbit IgG

    Application:
  • IF/ICC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab101265
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab101265-10μl
10μl
In stock
$49.90
Ab101265-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$147.90
Ab101265-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$237.90
Ab101265-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,217.90

Recombinant; Rabbit anti Human eIF2A Antibody; WB, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant eIF2A Antibody - Primary antibody, specific to EIF2A, Rabbit IgG
Synonyms65 kDa eukaryotic translation initiation factor 2A antibody | CDA 02 antibody | CDA02 antibody | EIF 2 antibody | EIF 2A antibody | eIF-2A antibody | EIF2 antibody | eif2a antibody | EIF2A_HUMAN antibody | Eukaryotic translation initiation factor 2A 65kDa
Specifications & PurityExactAb™, Validated, Recombinant, 0.5 mg/mL
Host speciesRabbit
SpecificityEIF2A
ImmunogenA synthetic peptide derived from human eIF2A (AA 480-494).
Positive ControlWB: MCF7, A549, HepG2, K562, A431 and NIH/3T3 cell lysates; ICC/IF: MCF7.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human eIF2A Antibody, Recombinant, could be used for WB and ICC/IF.
Application:
WB: 1/500 – 1/1000
ICC/IF: 1/50 – 1/200
Protein Function
Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant eIF2A Antibody (Ab101265) - Western Blot
All lanes: Recombinant eIF2A Antibody (Ab101265) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 65 kDa
Observed band size: 65&55 kDa
Exposure time: 1.7 s


Recombinant eIF2A Antibody (Ab101265) - IF
Immunocytochemistry analysis of eIF2A (green) in MCF7 using Recombinant eIF2A Antibody (Ab101265) and cells were counterstained with DAPI (blue).

Associated Targets(Human)

EIF2A Tbio Eukaryotic translation initiation factor 2A (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

IF/ICC 1/50-1/200

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1/50-1/200

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404260Certificate of AnalysisApr 09, 2024 Ab101265
ZJ23F0800791Certificate of AnalysisAug 14, 2023 Ab101265
ZJ23F0800792Certificate of AnalysisAug 14, 2023 Ab101265

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