Recombinant; Rabbit anti Human eIF4E Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab101380-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab101380-50μl | 50μl | In stock | $199.90 | |
| Ab101380-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab101380-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human eIF4E Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant eIF4E Antibody - Primary antibody, specific to EIF4E, Rabbit IgG |
|---|---|
| Synonyms | CBP; eIF4E; eIF-4E; EIF4E1; EIF4EL1; EIF4EL1MGC111573; eIF-4F 25 kDa subunit; EIF4F; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein; AUTS19 antibody; CBP antibody; eIF 4E antibody; |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | EIF4E |
| Immunogen | A synthetic peptide derived from human eIF4E (AA 176-194) |
| Positive Control | WB: HeLa, K562, A431, HEK293, C6 and NIH/3T3 lysates. IHC: Mouse colon tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human eIF4E Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant eIF4E Antibody (Ab101380) - Western Blot
All lanes: Recombinant eIF4E Antibody (Ab101380) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 27 kDa
Exposure time: 21.2 s
Recombinant eIF4E Antibody (Ab101380) - IHC
Immunohistochemistry analysis of paraffin-embedded mouse colon using Recombinant eIF4E Antibody (Ab101380). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1202239 | Certificate of Analysis | Dec 26, 2023 | Ab101380 |
| ZJ23F1202240 | Certificate of Analysis | Dec 26, 2023 | Ab101380 |
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| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Shuibin Lin,Junho Choe,Peng Du,Robinson Triboulet,Richard I Gregory. (2016-04-28) The m(6)A Methyltransferase METTL3 Promotes Translation in Human Cancer Cells.. Molecular cell, 62 ((3)): (335-345). [PMID:27117702] |
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| 18. Haghighat, A A, Mader, S S, Pause, A A and Sonenberg, N N.. (1995) Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor-4E.. The EMBO journal, (15): [PMID:8521827] |
| 19. Pyronnet, S S and 5 more authors.. (1999) Human eukaryotic translation initiation factor 4G (eIF4G) recruits mnk1 to phosphorylate eIF4E.. The EMBO journal, (4): [PMID:9878069] |
| 20. Dostie, J J, Ferraiuolo, M M, Pause, A A, Adam, S A SA and Sonenberg, N N.. (2000) A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E.. The EMBO journal, (15): [PMID:10856257] |
| 21. Scheper, G C GC, Morrice, N A NA, Kleijn, M M and Proud, C G CG.. (2001) The mitogen-activated protein kinase signal-integrating kinase Mnk2 is a eukaryotic initiation factor 4E kinase with high levels of basal activity in mammalian cells.. Molecular and cellular biology, [PMID:11154262] |
| 22. Li, W W, Belsham, G J GJ and Proud, C G CG.. (2001) Eukaryotic initiation factors 4A (eIF4A) and 4G (eIF4G) mutually interact in a 1:1 ratio in vivo.. The Journal of biological chemistry, (3): [PMID:11408474] |
| 23. Tomoo, Koji K and 15 more authors.. (2002) Crystal structures of 7-methylguanosine 5'-triphosphate (m(7)GTP)- and P(1)-7-methylguanosine-P(3)-adenosine-5',5'-triphosphate (m(7)GpppA)-bound human full-length eukaryotic initiation factor 4E: biological importance of the C-terminal flexible region.. The Biochemical journal, (15): [PMID:11879179] |
| 24. Scheper, Gert C GC and 6 more authors.. (2003) The N and C termini of the splice variants of the human mitogen-activated protein kinase-interacting kinase Mnk2 determine activity and localization.. Molecular and cellular biology, [PMID:12897141] |
| 25. Miura, Takaaki T, Shiratori, Yasuhiko Y and Shimma, Nobuo N.. (2003) Backbone resonance assignment of human eukaryotic translation initiation factor 4E (eIF4E) in complex with 7-methylguanosine diphosphate (m7GDP) and a 17-amino acid peptide derived from human eIF4GII.. Journal of biomolecular NMR, [PMID:12975586] |
| 26. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 27. Ferraiuolo, Maria A MA and 5 more authors.. (2005) A role for the eIF4E-binding protein 4E-T in P-body formation and mRNA decay.. The Journal of cell biology, (12): [PMID:16157702] |
| 28. Tomoo, Koji K and 9 more authors.. (2005) Structural basis for mRNA Cap-Binding regulation of eukaryotic initiation factor 4E by 4E-binding protein, studied by spectroscopic, X-ray crystal structural, and molecular dynamics simulation methods.. Biochimica et biophysica acta, (1): [PMID:16271312] |
| 29. Oh, Jung-Hwa JH and 12 more authors.. (2005) Transcriptome analysis of human gastric cancer.. Mammalian genome : official journal of the International Mammalian Genome Society, [PMID:16341674] |
| 30. Wichroski, Michael J MJ, Robb, G Brett GB and Rana, Tariq M TM.. (2006) Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies.. PLoS pathogens, [PMID:16699599] |
| 31. Brown, Christopher J CJ, McNae, Iain I, Fischer, Peter M PM and Walkinshaw, Malcolm D MD.. (2007) Crystallographic and mass spectrometric characterisation of eIF4E with N7-alkylated cap derivatives.. Journal of molecular biology, (7): [PMID:17631896] |
| 32. Shih, J-W JW, Tsai, T-Y TY, Chao, C-H CH and Wu Lee, Y-H YH.. (2008) Candidate tumor suppressor DDX3 RNA helicase specifically represses cap-dependent translation by acting as an eIF4E inhibitory protein.. Oncogene, (24): [PMID:17667941] |
| 33. Neves-Pereira, M M and 8 more authors.. (2009) Deregulation of EIF4E: a novel mechanism for autism.. Journal of medical genetics, [PMID:19556253] |
| 34. Volpon, Laurent L, Osborne, Michael J MJ, Capul, Althea A AA, de la Torre, Juan C JC and Borden, Katherine L B KL.. (2010) Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:20212144] |
| 35. Burrows, Carla C and 10 more authors.. (2010) The RNA binding protein Larp1 regulates cell division, apoptosis and cell migration.. Nucleic acids research, [PMID:20430826] |
| 36. James, Victoria V and 14 more authors.. (2010) LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing.. Proceedings of the National Academy of Sciences of the United States of America, (13): [PMID:20616046] |
| 37. Fukuyo, Ai A, In, Yasuko Y, Ishida, Toshimasa T and Tomoo, Koji K.. (2011) Structural scaffold for eIF4E binding selectivity of 4E-BP isoforms: crystal structure of eIF4E binding region of 4E-BP2 and its comparison with that of 4E-BP1.. Journal of peptide science : an official publication of the European Peptide Society, [PMID:21661078] |
| 38. Shih, Jing-Wen JW and 5 more authors.. (2012) Critical roles of RNA helicase DDX3 and its interactions with eIF4E/PABP1 in stress granule assembly and stress response.. The Biochemical journal, (1): [PMID:21883093] |
| 39. Yanagiya, Akiko A and 9 more authors.. (2012) Translational homeostasis via the mRNA cap-binding protein, eIF4E.. Molecular cell, (29): [PMID:22578813] |
| 40. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 41. Kubacka, Dorota D and 5 more authors.. (2013) Investigating the consequences of eIF4E2 (4EHP) interaction with 4E-transporter on its cellular distribution in HeLa cells.. PloS one, [PMID:23991149] |
| 42. Lukhele, Sabelo S, Bah, Alaji A, Lin, Hong H, Sonenberg, Nahum N and Forman-Kay, Julie D JD.. (2013) Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface.. Structure (London, England : 1993), (3): [PMID:24207126] |
| 43. Kamenska, Anastasiia A and 6 more authors.. (2014) Human 4E-T represses translation of bound mRNAs and enhances microRNA-mediated silencing.. Nucleic acids research, [PMID:24335285] |
| 44. Bah, Alaji A and 8 more authors.. (2015) Folding of an intrinsically disordered protein by phosphorylation as a regulatory switch.. Nature, (5): [PMID:25533957] |
| 45. Peter, Daniel D and 7 more authors.. (2015) Molecular architecture of 4E-BP translational inhibitors bound to eIF4E.. Molecular cell, (19): [PMID:25702871] |
| 46. Martínez, Alba and 6 more authors.. (2015) Phosphorylation of eIF4E Confers Resistance to Cellular Stress and DNA-Damaging Agents through an Interaction with 4E-T: A Rationale for Novel Therapeutic Approaches.. PloS one, [PMID:25923732] |
| 47. Chapat, Clément C and 12 more authors.. (2017) Cap-binding protein 4EHP effects translation silencing by microRNAs.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:28487484] |
| 48. Copsey, Alice C AC and 7 more authors.. (2017) The helicase, DDX3X, interacts with poly(A)-binding protein 1 (PABP1) and caprin-1 at the leading edge of migrating fibroblasts and is required for efficient cell spreading.. The Biochemical journal, (30): [PMID:28733330] |
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