| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab101380-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab101380-50μl | 50μl | In stock | $199.90 | |
| Ab101380-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab101380-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human eIF4E Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant eIF4E Antibody - Primary antibody, specific to EIF4E, Rabbit IgG |
|---|---|
| Synonyms | CBP | eIF4E | eIF-4E | EIF4E1 | EIF4EL1 | EIF4EL1MGC111573 | eIF-4F 25 kDa subunit | EIF4F | eukaryotic translation initiation factor 4E | eukaryotic translation initiation factor 4E-like 1 | mRNA cap-binding protein | AUTS19 antibody | CBP antibody | eIF |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | EIF4E |
| Immunogen | A synthetic peptide derived from human eIF4E (AA 176-194) |
| Positive Control | WB: HeLa, K562, A431, HEK293, C6 and NIH/3T3 lysates. IHC: Mouse colon tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human eIF4E Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant eIF4E Antibody (Ab101380) - Western Blot
All lanes: Recombinant eIF4E Antibody (Ab101380) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 27 kDa
Exposure time: 21.2 s
Recombinant eIF4E Antibody (Ab101380) - IHC
Immunohistochemistry analysis of paraffin-embedded mouse colon using Recombinant eIF4E Antibody (Ab101380). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1202239 | Certificate of Analysis | Dec 26, 2023 | Ab101380 |
| ZJ23F1202240 | Certificate of Analysis | Dec 26, 2023 | Ab101380 |
| 1. W Rychlik,M A Russ,R E Rhoads. (1987-08-05) Phosphorylation site of eukaryotic initiation factor 4E.. The Journal of biological chemistry, 262 ((22)): (10434-10437). [PMID:3112145] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Shuibin Lin,Junho Choe,Peng Du,Robinson Triboulet,Richard I Gregory. (2016-04-28) The m(6)A Methyltransferase METTL3 Promotes Translation in Human Cancer Cells.. Molecular cell, 62 ((3)): (335-345). [PMID:27117702] |
| 4. Sadovski O, Jaikaran AS, Samanta S, Fabian MR, Dowling RJ, Sonenberg N, Woolley GA.. (2010) A collection of caged compounds for probing roles of local translation in neurobiology.. Bioorg Med Chem, 18 (22): (7746-7752). [PMID:20427189] [10.1016/j.bmc.2010.04.005] |
| 5. Mahalingam P, Takrouri K, Chen T, Sahoo R, Papadopoulos E, Chen L, Wagner G, Aktas BH, Halperin JA, Chorev M.. (2014) Synthesis of rigidified eIF4E/eIF4G inhibitor-1 (4EGI-1) mimetic and their in vitro characterization as inhibitors of protein-protein interaction.. J Med Chem, 57 (12): (5094-5111). [PMID:24827861] [10.1021/jm401733v] |
| 6. Takrouri K, Chen T, Papadopoulos E, Sahoo R, Kabha E, Chen H, Cantel S, Wagner G, Halperin JA, Aktas BH, Chorev M.. (2014) Structure-activity relationship study of 4EGI-1, small molecule eIF4E/eIF4G protein-protein interaction inhibitors.. Eur J Med Chem, 77 (361-377). [PMID:24675136] [10.1016/j.ejmech.2014.03.034] |
| 7. Okon A, Han J, Dawadi S, Demosthenous C, Aldrich CC, Gupta M, Wagner CR.. (2017) Anchimerically Activated ProTides as Inhibitors of Cap-Dependent Translation and Inducers of Chemosensitization in Mantle Cell Lymphoma.. J Med Chem, 60 (19): (8131-8144). [PMID:28858511] [10.1021/acs.jmedchem.7b00916] |
| 8. Lorenz DA, Kaur T, Kerk SA, Gallagher EE, Sandoval J, Garner AL.. (2018) Expansion of cat-ELCCA for the Discovery of Small Molecule Inhibitors of the Pre-let-7-Lin28 RNA-Protein Interaction.. ACS Med Chem Lett, 9 (6): (517-521). [PMID:29937975] [10.1021/acsmedchemlett.8b00126] |
| 9. Kaur T,Menon A,Garner AL. (2019) Synthesis of 7-benzylguanosine cap-analogue conjugates for eIF4E targeted degradation.. Eur J Med Chem, 166 (339-350). [PMID:30735900] [10.1016/j.ejmech.2019.01.080] |
| 10. Ueda, H H and 8 more authors.. (1991) Combination of Trp and Glu residues for recognition of mRNA cap structure. Analysis of m7G base recognition site of human cap binding protein (IF-4E) by site-directed mutagenesis.. FEBS letters, (25): [PMID:1672854] |
| 11. Rychlik, W W, Domier, L L LL, Gardner, P R PR, Hellmann, G M GM and Rhoads, R E RE.. (1992) Amino acid sequence of the mRNA cap-binding protein from human tissues.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1736299] |
| 12. Marino, M W MW and 5 more authors.. (1991) Phosphorylation of the proto-oncogene product eukaryotic initiation factor 4E is a common cellular response to tumor necrosis factor.. The Journal of biological chemistry, (15): [PMID:1993647] |
| 13. Rychlik, W W, Domier, L L LL, Gardner, P R PR, Hellmann, G M GM and Rhoads, R E RE.. (1987) Amino acid sequence of the mRNA cap-binding protein from human tissues.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3469651] |
| 14. Zhang, Y Y, Klein, H L HL and Schneider, R J RJ.. (1995) Role of Ser-53 phosphorylation in the activity of human translation initiation factor eIF-4E in mammalian and yeast cells.. Gene, (3): [PMID:7590282] |
| 15. Flynn, A A and Proud, C G CG.. (1995) Serine 209, not serine 53, is the major site of phosphorylation in initiation factor eIF-4E in serum-treated Chinese hamster ovary cells.. The Journal of biological chemistry, (15): [PMID:7665584] |
| 16. Joshi, B B and 9 more authors.. (1995) Phosphorylation of eukaryotic protein synthesis initiation factor 4E at Ser-209.. The Journal of biological chemistry, (16): [PMID:7782323] |
| 17. Kaufman, R J RJ, Murtha-Riel, P P, Pittman, D D DD and Davies, M V MV.. (1993) Characterization of wild-type and Ser53 mutant eukaryotic initiation factor 4E overexpression in mammalian cells.. The Journal of biological chemistry, (5): [PMID:8505316] |
| 18. Haghighat, A A, Mader, S S, Pause, A A and Sonenberg, N N.. (1995) Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor-4E.. The EMBO journal, (15): [PMID:8521827] |
| 19. Pyronnet, S S and 5 more authors.. (1999) Human eukaryotic translation initiation factor 4G (eIF4G) recruits mnk1 to phosphorylate eIF4E.. The EMBO journal, (4): [PMID:9878069] |
| 20. Dostie, J J, Ferraiuolo, M M, Pause, A A, Adam, S A SA and Sonenberg, N N.. (2000) A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E.. The EMBO journal, (15): [PMID:10856257] |
| 21. Scheper, G C GC, Morrice, N A NA, Kleijn, M M and Proud, C G CG.. (2001) The mitogen-activated protein kinase signal-integrating kinase Mnk2 is a eukaryotic initiation factor 4E kinase with high levels of basal activity in mammalian cells.. Molecular and cellular biology, [PMID:11154262] |
| 22. Li, W W, Belsham, G J GJ and Proud, C G CG.. (2001) Eukaryotic initiation factors 4A (eIF4A) and 4G (eIF4G) mutually interact in a 1:1 ratio in vivo.. The Journal of biological chemistry, (3): [PMID:11408474] |
| 23. Tomoo, Koji K and 15 more authors.. (2002) Crystal structures of 7-methylguanosine 5'-triphosphate (m(7)GTP)- and P(1)-7-methylguanosine-P(3)-adenosine-5',5'-triphosphate (m(7)GpppA)-bound human full-length eukaryotic initiation factor 4E: biological importance of the C-terminal flexible region.. The Biochemical journal, (15): [PMID:11879179] |
| 24. Scheper, Gert C GC and 6 more authors.. (2003) The N and C termini of the splice variants of the human mitogen-activated protein kinase-interacting kinase Mnk2 determine activity and localization.. Molecular and cellular biology, [PMID:12897141] |
| 25. Miura, Takaaki T, Shiratori, Yasuhiko Y and Shimma, Nobuo N.. (2003) Backbone resonance assignment of human eukaryotic translation initiation factor 4E (eIF4E) in complex with 7-methylguanosine diphosphate (m7GDP) and a 17-amino acid peptide derived from human eIF4GII.. Journal of biomolecular NMR, [PMID:12975586] |
| 26. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 27. Ferraiuolo, Maria A MA and 5 more authors.. (2005) A role for the eIF4E-binding protein 4E-T in P-body formation and mRNA decay.. The Journal of cell biology, (12): [PMID:16157702] |
| 28. Tomoo, Koji K and 9 more authors.. (2005) Structural basis for mRNA Cap-Binding regulation of eukaryotic initiation factor 4E by 4E-binding protein, studied by spectroscopic, X-ray crystal structural, and molecular dynamics simulation methods.. Biochimica et biophysica acta, (1): [PMID:16271312] |
| 29. Oh, Jung-Hwa JH and 12 more authors.. (2005) Transcriptome analysis of human gastric cancer.. Mammalian genome : official journal of the International Mammalian Genome Society, [PMID:16341674] |
| 30. Wichroski, Michael J MJ, Robb, G Brett GB and Rana, Tariq M TM.. (2006) Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies.. PLoS pathogens, [PMID:16699599] |
| 31. Brown, Christopher J CJ, McNae, Iain I, Fischer, Peter M PM and Walkinshaw, Malcolm D MD.. (2007) Crystallographic and mass spectrometric characterisation of eIF4E with N7-alkylated cap derivatives.. Journal of molecular biology, (7): [PMID:17631896] |
| 32. Shih, J-W JW, Tsai, T-Y TY, Chao, C-H CH and Wu Lee, Y-H YH.. (2008) Candidate tumor suppressor DDX3 RNA helicase specifically represses cap-dependent translation by acting as an eIF4E inhibitory protein.. Oncogene, (24): [PMID:17667941] |
| 33. Neves-Pereira, M M and 8 more authors.. (2009) Deregulation of EIF4E: a novel mechanism for autism.. Journal of medical genetics, [PMID:19556253] |
| 34. Volpon, Laurent L, Osborne, Michael J MJ, Capul, Althea A AA, de la Torre, Juan C JC and Borden, Katherine L B KL.. (2010) Structural characterization of the Z RING-eIF4E complex reveals a distinct mode of control for eIF4E.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:20212144] |
| 35. Burrows, Carla C and 10 more authors.. (2010) The RNA binding protein Larp1 regulates cell division, apoptosis and cell migration.. Nucleic acids research, [PMID:20430826] |
| 36. James, Victoria V and 14 more authors.. (2010) LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing.. Proceedings of the National Academy of Sciences of the United States of America, (13): [PMID:20616046] |
| 37. Fukuyo, Ai A, In, Yasuko Y, Ishida, Toshimasa T and Tomoo, Koji K.. (2011) Structural scaffold for eIF4E binding selectivity of 4E-BP isoforms: crystal structure of eIF4E binding region of 4E-BP2 and its comparison with that of 4E-BP1.. Journal of peptide science : an official publication of the European Peptide Society, [PMID:21661078] |
| 38. Shih, Jing-Wen JW and 5 more authors.. (2012) Critical roles of RNA helicase DDX3 and its interactions with eIF4E/PABP1 in stress granule assembly and stress response.. The Biochemical journal, (1): [PMID:21883093] |
| 39. Yanagiya, Akiko A and 9 more authors.. (2012) Translational homeostasis via the mRNA cap-binding protein, eIF4E.. Molecular cell, (29): [PMID:22578813] |
| 40. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 41. Kubacka, Dorota D and 5 more authors.. (2013) Investigating the consequences of eIF4E2 (4EHP) interaction with 4E-transporter on its cellular distribution in HeLa cells.. PloS one, [PMID:23991149] |
| 42. Lukhele, Sabelo S, Bah, Alaji A, Lin, Hong H, Sonenberg, Nahum N and Forman-Kay, Julie D JD.. (2013) Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface.. Structure (London, England : 1993), (3): [PMID:24207126] |
| 43. Kamenska, Anastasiia A and 6 more authors.. (2014) Human 4E-T represses translation of bound mRNAs and enhances microRNA-mediated silencing.. Nucleic acids research, [PMID:24335285] |
| 44. Bah, Alaji A and 8 more authors.. (2015) Folding of an intrinsically disordered protein by phosphorylation as a regulatory switch.. Nature, (5): [PMID:25533957] |
| 45. Peter, Daniel D and 7 more authors.. (2015) Molecular architecture of 4E-BP translational inhibitors bound to eIF4E.. Molecular cell, (19): [PMID:25702871] |
| 46. Martínez, Alba and 6 more authors.. (2015) Phosphorylation of eIF4E Confers Resistance to Cellular Stress and DNA-Damaging Agents through an Interaction with 4E-T: A Rationale for Novel Therapeutic Approaches.. PloS one, [PMID:25923732] |
| 47. Chapat, Clément C and 12 more authors.. (2017) Cap-binding protein 4EHP effects translation silencing by microRNAs.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:28487484] |
| 48. Copsey, Alice C AC and 7 more authors.. (2017) The helicase, DDX3X, interacts with poly(A)-binding protein 1 (PABP1) and caprin-1 at the leading edge of migrating fibroblasts and is required for efficient cell spreading.. The Biochemical journal, (30): [PMID:28733330] |
| 49. Zhang, Min M and 7 more authors.. (2018) Rbm24, a target of p53, is necessary for proper expression of p53 and heart development.. Cell death and differentiation, [PMID:29358667] |
| 50. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
