| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab101502-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab101502-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $207.90 | |
| Ab101502-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $297.90 | |
| Ab101502-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,697.90 |
Recombinant; Rabbit anti Human ELK1 Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant ELK1 Antibody - Primary antibody, specific to ELK1, Rabbit IgG |
|---|---|
| Synonyms | ETS domain-containing protein Elk-1 | ELK 1 antibody | Elk1 antibody | ELK1 member of ETS oncogene family antibody | ELK1 protein antibody | ELK1, ETS transcription factor antibody | ELK1_HUMAN antibody | ELK2 member of ETS oncogene family antibody | ETS |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | ELK1 |
| Immunogen | A synthetic peptide derived from human ELK1 (AA 408-428). |
| Positive Control | WB: K562,HT29, A431,MCF7 cell lysates; IHC: Human lung cancer tissues. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human ELK1 Antibody, Recombinant, could be used for WB IHC and so on. Application:
Protein Function Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant ELK1 Antibody (Ab101502) - Western Blot
All lanes: Recombinant ELK1 Antibody (Ab101502) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 62 kDa
Exposure time: 20.0 s
Recombinant ELK1 Antibody (Ab101502) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung using Recombinant ELK1 Antibody (Ab101502). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0800816 | Certificate of Analysis | Aug 16, 2023 | Ab101502 |
| ZJ23F0800815 | Certificate of Analysis | Aug 16, 2023 | Ab101502 |
| 1. Li Q, Al-Ayoubi A, Guo T, Zheng H, Sarkar A, Nguyen T, Eblen ST, Grant S, Kellogg GE, Zhang S.. (2009) Structure-activity relationship (SAR) studies of 3-(2-amino-ethyl)-5-(4-ethoxy-benzylidene)-thiazolidine-2,4-dione: development of potential substrate-specific ERK1/2 inhibitors.. Bioorg Med Chem Lett, 19 (21): (6042-6046). [PMID:19796943] [10.1016/j.bmcl.2009.09.057] |
| 2. Janknecht, R R and Nordheim, A A.. (1992) Elk-1 protein domains required for direct and SRF-assisted DNA-binding.. Nucleic acids research, (11): [PMID:1630903] |
| 3. Rao, V N VN and 5 more authors.. (1989) elk, tissue-specific ets-related genes on chromosomes X and 14 near translocation breakpoints.. Science (New York, N.Y.), (7): [PMID:2539641] |
| 4. Gille, H H and 6 more authors.. (1995) ERK phosphorylation potentiates Elk-1-mediated ternary complex formation and transactivation.. The EMBO journal, (1): [PMID:7889942] |
| 5. Enslen, H H, Tokumitsu, H H, Stork, P J PJ, Davis, R J RJ and Soderling, T R TR.. (1996) Regulation of mitogen-activated protein kinases by a calcium/calmodulin-dependent protein kinase cascade.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:8855261] |
| 6. Enslen, H H, Raingeaud, J J and Davis, R J RJ.. (1998) Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6.. The Journal of biological chemistry, (16): [PMID:9430721] |
| 7. Harindranath, N N, Mills, F C FC, Mitchell, M M, Meindl, A A and Max, E E EE.. (1998) The human elk-1 gene family: the functional gene and two processed pseudogenes embedded in the IgH locus.. Gene, (23): [PMID:9795224] |
| 8. Yamauchi, T T, Toko, M M, Suga, M M, Hatakeyama, T T and Isobe, M M.. (1999) Structural organization of the human Elk1 gene and its processed pseudogene Elk2.. DNA research : an international journal for rapid publication of reports on genes and genomes, (26): [PMID:10231026] |
| 9. Mo, Y Y, Vaessen, B B, Johnston, K K and Marmorstein, R R.. (2000) Structure of the elk-1-DNA complex reveals how DNA-distal residues affect ETS domain recognition of DNA.. Nature structural biology, [PMID:10742173] |
| 10. Yang, Shen-Hsi SH, Jaffray, Ellis E, Hay, Ron T RT and Sharrocks, Andrew D AD.. (2003) Dynamic interplay of the SUMO and ERK pathways in regulating Elk-1 transcriptional activity.. Molecular cell, [PMID:12887893] |
| 11. Yang, Shen-Hsi SH and Sharrocks, Andrew D AD.. (2004) SUMO promotes HDAC-mediated transcriptional repression.. Molecular cell, (27): [PMID:14992729] |
| 12. Salinas, Sara S and 7 more authors.. (2004) SUMOylation regulates nucleo-cytoplasmic shuttling of Elk-1.. The Journal of cell biology, (21): [PMID:15210726] |
| 13. Ross, Mark T MT and 281 more authors.. (2005) The DNA sequence of the human X chromosome.. Nature, (17): [PMID:15772651] |
| 14. Yang, Shen-Hsi SH and Sharrocks, Andrew D AD.. (2005) PIASx acts as an Elk-1 coactivator by facilitating derepression.. The EMBO journal, (15): [PMID:15920481] |
| 15. Zhang, Lin L, Yang, Shen-Hsi SH and Sharrocks, Andrew D AD.. (2007) Rev7/MAD2B links c-Jun N-terminal protein kinase pathway signaling to activation of the transcription factor Elk-1.. Molecular and cellular biology, [PMID:17296730] |
| 16. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 17. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 18. Li, Qing-Shan QS and 6 more authors.. (2012) Synthesis and biological evaluation of novel N, N'-disubstituted urea and thiourea derivatives as potential anti-melanoma agents.. Journal of enzyme inhibition and medicinal chemistry, [PMID:21985377] |
| 19. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 20. Sobrier, Marie-Laure ML and 12 more authors.. (2016) Functional characterization of a human POU1F1 mutation associated with isolated growth hormone deficiency: a novel etiology for IGHD.. Human molecular genetics, (1): [PMID:26612202] |
