| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab101516-10μl | 10μl | In stock | $66.90 | |
| Ab101516-50μl | 50μl | In stock | $199.90 | |
| Ab101516-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab101516-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human ELMO1 Antibody; WB, IF, ICC; Unconjugated
| Product Name | Recombinant ELMO1 Antibody - Primary antibody, specific to ELMO1, Rabbit IgG |
|---|---|
| Synonyms | CED 12 | Ced 12 homolog 1 | Ced 12 homolog | CED12 | Ced12 homolog 1 | Ced12 homolog | ELMO 1 | Engulfment and cell motility 1 | Engulfment and cell motility protein 1 | KIAA0281 | ELMO1_HUMAN | CED 12 antibody | Ced 12 homolog 1 antibody | Ced 12 homolog |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.3 mg/mL |
| Host species | Rabbit |
| Specificity | ELMO1 |
| Immunogen | A synthetic peptide derived from human ELMO1 (AA 712-726) |
| Positive Control | WB: Raji, HUVEC, NIH/3T3 and C6 cell lysates. ICC/IF: Jurkat cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human ELMO1 Antibody, Recombinant, could be used for WB, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant ELMO1 Antibody (Ab101516) - Western Blot
All lanes: Recombinant ELMO1 Antibody (Ab101516) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 84 kDa
Observed band size: 80 kDa
Exposure time: 20.0 s
Recombinant ELMO1 Antibody (Ab101516) - Western Blot
All lanes: Recombinant ELMO1 Antibody (Ab101516) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 84 kDa
Observed band size: 80 kDa
Exposure time: 40.0 s
Recombinant ELMO1 Antibody (Ab101516) - IF/ICC
Immunocytochemistry analysis of ELMO1 (green) in Jurkat using Recombinant ELMO1 Antibody (Ab101516), and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303342 | Certificate of Analysis | Mar 18, 2024 | Ab101516 |
| ZJ24F0303343 | Certificate of Analysis | Mar 18, 2024 | Ab101516 |
| 1. Scherer SW, Cheung J, MacDonald JR, Osborne LR, Nakabayashi K, Herbrick JA, Carson AR, Parker-Katiraee L, Skaug J, Khaja R et al.. (2003) Human chromosome 7: DNA sequence and biology.. Science, 300 (5620): (767-72). [PMID:12690205] [10.1021/op500134e] |
| 2. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 3. Hamoud N, Tran V, Croteau LP, Kania A, Côté JF. (2014) G-protein coupled receptor BAI3 promotes myoblast fusion in vertebrates.. Proc Natl Acad Sci USA, 111 (10): (3745-50). [PMID:24567399] [10.1021/op500134e] |
| 4. Ohara, O O and 6 more authors.. (1997) Construction and characterization of human brain cDNA libraries suitable for analysis of cDNA clones encoding relatively large proteins.. DNA research : an international journal for rapid publication of reports on genes and genomes, (28): [PMID:9179496] |
| 5. Wiemann, S S and 23 more authors.. (2001) Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.. Genome research, [PMID:11230166] |
| 6. Gumienny, T L TL and 14 more authors.. (2001) CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration.. Cell, (5): [PMID:11595183] |
| 7. Scott, Margaret Porter MP, Zappacosta, Francesca F, Kim, Eun Young EY, Annan, Roland S RS and Miller, W Todd WT.. (2002) Identification of novel SH3 domain ligands for the Src family kinase Hck. Wiskott-Aldrich syndrome protein (WASP), WASP-interacting protein (WIP), and ELMO1.. The Journal of biological chemistry, (2): [PMID:12029088] |
| 8. Brugnera, Enrico E and 9 more authors.. (2002) Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex.. Nature cell biology, [PMID:12134158] |
| 9. Yokoyama, Noriko N and 6 more authors.. (2005) Identification of tyrosine residues on ELMO1 that are phosphorylated by the Src-family kinase Hck.. Biochemistry, (21): [PMID:15952790] |
| 10. D'Angelo, Romina R and 5 more authors.. (2007) Interaction of ezrin with the novel guanine nucleotide exchange factor PLEKHG6 promotes RhoG-dependent apical cytoskeleton rearrangements in epithelial cells.. Molecular biology of the cell, [PMID:17881735] |
| 11. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 12. Funari, Vincent A VA and 9 more authors.. (2010) BMPER mutation in diaphanospondylodysostosis identified by ancestral autozygosity mapping and targeted high-throughput sequencing.. American journal of human genetics, (8): [PMID:20869035] |
| 13. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
