Rabbit anti Human ELMO1 Antibody, Recombinant, could be used for WB, IF, ICC and so on. Application: WB: 1/500-1/1000 IF: 1/50-1/200 Protein Function Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.3 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant ELMO1 Antibody (Ab101516) - Western Blot All lanes: Recombinant ELMO1 Antibody (Ab101516) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 84 kDa Observed band size: 80 kDa Exposure time: 20.0 s
Recombinant ELMO1 Antibody (Ab101516) - Western Blot All lanes: Recombinant ELMO1 Antibody (Ab101516) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 84 kDa Observed band size: 80 kDa Exposure time: 40.0 s
Recombinant ELMO1 Antibody (Ab101516) - IF/ICC Immunocytochemistry analysis of ELMO1 (green) in Jurkat using Recombinant ELMO1 Antibody (Ab101516), and cells were counterstained with DAPI (blue).
Associated Targets
ELMO1Tbio Engulfment and cell motility protein 1 0 Activities
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3.Hamoud N, Tran V, Croteau LP, Kania A, Côté JF. (2014) G-protein coupled receptor BAI3 promotes myoblast fusion in vertebrates.. Proc Natl Acad Sci USA, 111 (10):(3745-50). [PMID:24567399]
4.Ohara, O O and 6 more authors.. (1997) Construction and characterization of human brain cDNA libraries suitable for analysis of cDNA clones encoding relatively large proteins.. DNA research : an international journal for rapid publication of reports on genes and genomes, (28):[PMID:9179496]
5.Wiemann, S S and 23 more authors.. (2001) Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.. Genome research, [PMID:11230166]
6.Gumienny, T L TL and 14 more authors.. (2001) CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration.. Cell, (5):[PMID:11595183]
7.Scott, Margaret Porter MP, Zappacosta, Francesca F, Kim, Eun Young EY, Annan, Roland S RS and Miller, W Todd WT.. (2002) Identification of novel SH3 domain ligands for the Src family kinase Hck. Wiskott-Aldrich syndrome protein (WASP), WASP-interacting protein (WIP), and ELMO1.. The Journal of biological chemistry, (2):[PMID:12029088]
8.Brugnera, Enrico E and 9 more authors.. (2002) Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex.. Nature cell biology, [PMID:12134158]
9.Yokoyama, Noriko N and 6 more authors.. (2005) Identification of tyrosine residues on ELMO1 that are phosphorylated by the Src-family kinase Hck.. Biochemistry, (21):[PMID:15952790]
10.D'Angelo, Romina R and 5 more authors.. (2007) Interaction of ezrin with the novel guanine nucleotide exchange factor PLEKHG6 promotes RhoG-dependent apical cytoskeleton rearrangements in epithelial cells.. Molecular biology of the cell, [PMID:17881735]
11.Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18):[PMID:19690332]
12.Funari, Vincent A VA and 9 more authors.. (2010) BMPER mutation in diaphanospondylodysostosis identified by ancestral autozygosity mapping and targeted high-throughput sequencing.. American journal of human genetics, (8):[PMID:20869035]
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