Recombinant; Rabbit anti Human ENPP5 Antibody; WB, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab101745-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab101745-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab101745-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $319.90 | |
| Ab101745-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
Recombinant; Rabbit anti Human ENPP5 Antibody; WB, ICC, IF; Unconjugated
| Product Name | Recombinant ENPP5 Antibody |
|---|---|
| Specifications & Purity | Recombinant, ExactAb™, Validated, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | ENPP5 |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within human ENPP5. |
| Positive Control | WB: HEK293, MCF7, HeLa, Hep G2, NIH/3T3 and C6 cell lysates. ICC/IF: COS7 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human ENPP5 Antibody, Recombinant, could be used for WB, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant ENPP5 Antibody (Ab101745) - Western Blot
All lanes: Recombinant ENPP5 Antibody (Ab101745) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa
Exposure time: 60.0 s
Recombinant ENPP5 Antibody (Ab101745) - Western Blot
All lanes: Recombinant ENPP5 Antibody (Ab101745) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 51 kDa
Exposure time: 100.0 s
Recombinant ENPP5 Antibody (Ab101745) - IF/ICC
Immunofluorescent analysis of ENPP5 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 mintes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the Recombinant ENPP5 Antibody (Ab101745) in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| ICC/IF |
1/50-1/200
|
| WB |
1/500-1/1000
|
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