KLH-conjugated synthetic peptide encompassing a sequence within human ENPP5.
Positive Control
WB: HEK293, MCF7, HeLa, Hep G2, NIH/3T3 and C6 cell lysates. ICC/IF: COS7 cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human ENPP5 Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function: May play a role in neuronal cell communication. Lacks nucleotide pyrophosphatase and lysopholipase D activity.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Form
Liquid
Concentration
0.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant ENPP5 Antibody (Ab101745) - Western Blot All lanes: Recombinant ENPP5 Antibody (Ab101745) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 60.0 s
Recombinant ENPP5 Antibody (Ab101745) - Western Blot All lanes: Recombinant ENPP5 Antibody (Ab101745) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa Observed band size: 51 kDa Exposure time: 100.0 s
Recombinant ENPP5 Antibody (Ab101745) - IF/ICC Immunofluorescent analysis of ENPP5 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 mintes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the Recombinant ENPP5 Antibody (Ab101745) in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Associated Targets(Human)
ENPP5TbioEctonucleotide pyrophosphatase/phosphodiesterase family member 5 (0 Activities)