Recombinant; Rabbit anti Human ENT2 Antibody; WB, IHC, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab101760-10μl | 10μl | In stock | $89.90 | |
| Ab101760-50μl | 50μl | In stock | $269.90 | |
| Ab101760-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $379.90 | |
| Ab101760-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Recombinant; Rabbit anti Human ENT2 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Recombinant ENT2 Antibody - Primary antibody, specific to SLC29A2, Rabbit IgG |
|---|---|
| Synonyms | DER12 | Equilibrative NBMPR-insensitive nucleoside transporter | Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter | Equilibrative nucleoside transporter 2 | HNP36 | Hydrophobic nucleolar protein, 36 kDa | SLC29A2 | solut |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.3 mg/mL |
| Host species | Rabbit |
| Specificity | SLC29A2 |
| Immunogen | A synthetic peptide derived from human ENT2 (AA 1-12) |
| Positive Control | WB: HeLa, K562, Hep G2, Jurkat and NIH/3T3 cell lysates. IHC: Human breast cancer tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human ENT2 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant ENT2 Antibody (Ab101760) - Western Blot
All lanes: Recombinant ENT2 Antibody (Ab101760) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 62, 70 kDa
Exposure time: 20.0 s
Recombinant ENT2 Antibody (Ab101760) - IF/ICC
Immunocytochemistry analysis of ENT2 (green) in HeLa using Recombinant ENT2 Antibody (Ab101760), and cells were counterstained with DAPI (blue).
Recombinant ENT2 Antibody (Ab101760) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant ENT2 Antibody (Ab101760). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303336 | Certificate of Analysis | Mar 18, 2024 | Ab101760 |
| ZJ24F0303337 | Certificate of Analysis | Mar 18, 2024 | Ab101760 |
| 1. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Williams, J B JB and Lanahan, A A AA.. (1995) A mammalian delayed-early response gene encodes HNP36, a novel, conserved nucleolar protein.. Biochemical and biophysical research communications, (4): [PMID:7639753] |
| 4. Griffiths, M M and 6 more authors.. (1997) Molecular cloning and characterization of a nitrobenzylthioinosine-insensitive (ei) equilibrative nucleoside transporter from human placenta.. The Biochemical journal, (15): [PMID:9396714] |
| 5. Crawford, C R CR, Patel, D H DH, Naeve, C C and Belt, J A JA.. (1998) Cloning of the human equilibrative, nitrobenzylmercaptopurine riboside (NBMPR)-insensitive nucleoside transporter ei by functional expression in a transport-deficient cell line.. The Journal of biological chemistry, (27): [PMID:9478986] |
| 6. Mangravite, Lara M LM, Xiao, Guangqing G and Giacomini, Kathleen M KM.. (2003) Localization of human equilibrative nucleoside transporters, hENT1 and hENT2, in renal epithelial cells.. American journal of physiology. Renal physiology, [PMID:12527552] |
| 7. Ward, Jeffrey L JL, Leung, George P H GP, Toan, Shuy-Vang SV and Tse, Chung-Ming CM.. (2003) Functional analysis of site-directed glycosylation mutants of the human equilibrative nucleoside transporter-2.. Archives of biochemistry and biophysics, (1): [PMID:12590919] |
| 8. Owen, Ryan P RP and 9 more authors.. (2006) Functional characterization and haplotype analysis of polymorphisms in the human equilibrative nucleoside transporter, ENT2.. Drug metabolism and disposition: the biological fate of chemicals, [PMID:16214850] |
| 9. Allen-Gipson, Diane S and 6 more authors.. (2009) Ethanol blocks adenosine uptake via inhibiting the nucleoside transport system in bronchial epithelial cells.. Alcoholism, clinical and experimental research, [PMID:19298329] |
| 10. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 11. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
