Recombinant (RR694); Rabbit anti Human EpCAM Antibody; WB, IHC, Flow, IP; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab101839-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab101839-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $177.90 | |
| Ab101839-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $297.90 | |
| Ab101839-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,397.90 |
Recombinant (RR694); Rabbit anti Human EpCAM Antibody; WB, IHC, Flow, IP; Unconjugated
| Product Name | Recombinant EpCAM Antibody - Primary antibody, specific to EpCAM, Rabbit IgG |
|---|---|
| Synonyms | 17 1A antibody | 323/A3 antibody | Adenocarcinoma associated antigen antibody | Adenocarcinoma-associated antigen antibody | Antigen identified by monoclonal antibody AUA1 antibody | AUA1 antibody | CD326 antibody | CD326 antigen antibody | Cell surface g |
| Specifications & Purity | ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | EpCAM |
| Immunogen | A synthetic peptide corresponding to residues of human EPCAM. (AA 1-100) |
| Positive Control | WB: Hep G2, HEK293 and A431 cell lysates. IHC: Human Uterus tissue. Flow: HCT-116 cells. IP: HT-29 whole cell lysate. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Recombinant, Validated |
| Product Description | Rabbit anti Human EpCAM Antibody, Recombinant antibody (RR694), could be used for WB, IHC, Flow and IP. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant EpCAM Antibody (Ab101839) - Western Blot
All lanes: Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 39 kDa
Exposure time: 5.0 s
Recombinant EpCAM Antibody (Ab101839) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus tissue labelling EpCAM with Recombinant EpCAM Antibody (Ab101839) at 1/3200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant EpCAM Antibody (Ab101839) - Flow Cytometry
Overlay histogram showing HCT-116 cells stained with Recombinant EpCAM Antibody (Ab101839) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant EpCAM Antibody (Ab101839) ( 1/800 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
Recombinant EpCAM Antibody (Ab101839) - IP
EpCAM was immunoprecipitated from 0.4 mg of HT-29 whole cell lysate with Recombinant EpCAM Antibody (Ab101839) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant EpCAM Antibody (Ab101839) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant EpCAM Antibody (Ab101839) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)
Exposure: 120 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| IP |
1/50
|
| Flow Cytometry | 1/800 - 1/2000 |
| WB |
1/2000 - 1/5000
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0901018 | Certificate of Analysis | Sep 11, 2023 | Ab101839 |
| ZJ23F0901017 | Certificate of Analysis | Sep 11, 2023 | Ab101839 |
| 1. Simon, B B and 5 more authors.. (1990) Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2108441] |
| 2. Szala, S S and 6 more authors.. (1990) Molecular cloning of cDNA for the carcinoma-associated antigen GA733-2.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2333300] |
| 3. Strnad, J J and 9 more authors.. (1989) Molecular cloning and characterization of a human adenocarcinoma/epithelial cell surface antigen complementary DNA.. Cancer research, (15): [PMID:2463074] |
| 4. Perez, M S MS and Walker, L E LE.. (1989) Isolation and characterization of a cDNA encoding the KS1/4 epithelial carcinoma marker.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:2469722] |
| 5. Linnenbach, A J AJ and 7 more authors.. (1989) Sequence investigation of the major gastrointestinal tumor-associated antigen gene family, GA733.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2911574] |
| 6. Björk, P P and 8 more authors.. (1993) Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody.. The Journal of biological chemistry, (15): [PMID:7693697] |
| 7. Linnenbach, A J AJ and 7 more authors.. (1993) Retroposition in a family of carcinoma-associated antigen genes.. Molecular and cellular biology, [PMID:8382772] |
| 8. Chong, J M JM and Speicher, D W DW.. (2001) Determination of disulfide bond assignments and N-glycosylation sites of the human gastrointestinal carcinoma antigen GA733-2 (CO17-1A, EGP, KS1-4, KSA, and Ep-CAM).. The Journal of biological chemistry, (23): [PMID:11080501] |
| 9. Münz, Markus M and 5 more authors.. (2004) The carcinoma-associated antigen EpCAM upregulates c-myc and induces cell proliferation.. Oncogene, (29): [PMID:15195135] |
| 10. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 11. Münz, Markus M, Zeidler, Reinhard R and Gires, Olivier O.. (2005) The tumour-associated antigen EpCAM upregulates the fatty acid binding protein E-FABP.. Cancer letters, (8): [PMID:15922867] |
| 12. Ladwein, Markus M and 8 more authors.. (2005) The cell-cell adhesion molecule EpCAM interacts directly with the tight junction protein claudin-7.. Experimental cell research, (1): [PMID:16054130] |
| 13. Munz, Markus M, Fellinger, Karin K, Hofmann, Tanja T, Schmitt, Barbel B and Gires, Olivier O.. (2008) Glycosylation is crucial for stability of tumour and cancer stem cell antigen EpCAM.. Frontiers in bioscience : a journal and virtual library, (1): [PMID:18508581] |
| 14. Sivagnanam, Mamata M and 15 more authors.. (2008) Identification of EpCAM as the gene for congenital tufting enteropathy.. Gastroenterology, [PMID:18572020] |
| 15. Ligtenberg, Marjolijn J L MJ and 17 more authors.. (2009) Heritable somatic methylation and inactivation of MSH2 in families with Lynch syndrome due to deletion of the 3' exons of TACSTD1.. Nature genetics, [PMID:19098912] |
| 16. Ng, Valerie Y VY, Ang, Sheu Ngo SN, Chan, Jia Xin JX and Choo, Andre B H AB.. (2010) Characterization of epithelial cell adhesion molecule as a surface marker on undifferentiated human embryonic stem cells.. Stem cells (Dayton, Ohio), [PMID:19785009] |
| 17. Lu, Tung-Ying TY and 6 more authors.. (2010) Epithelial cell adhesion molecule regulation is associated with the maintenance of the undifferentiated phenotype of human embryonic stem cells.. The Journal of biological chemistry, (19): [PMID:20064925] |
| 18. Seimetz, Diane D, Lindhofer, Horst H and Bokemeyer, Carsten C.. (2010) Development and approval of the trifunctional antibody catumaxomab (anti-EpCAM x anti-CD3) as a targeted cancer immunotherapy.. Cancer treatment reviews, [PMID:20347527] |
| 19. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
