ExactAb™, Validated, Recombinant, High performance, 0.103 mg/mL
Host species
Rabbit
Specificity
Recognizes natural and recombinant human FGF2
Immunogen
Synthetic peptide within Human FGF2 (AA: 100-200).
Positive Control
WB: HeLa, A549 and HUVEC cell lysates. IHC: Human prostate cancer tissue.
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Recombinant, Validated
Product Description
Rabbit anti Human FGF2 Antibody, Recombinant (EP1735), could be used for WB, IHC, IP and Sandwich ELISA. Application IHC: 1/100-1/200 WB: 1/1000 - 1/10000 IP: 1/10 - 1/100 Sandwich ELISA: 1/200 Protein Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.103 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant FGF2 Antibody (Ab007066) - Western Blot All lanes: Recombinant FGF2 Antibody (Ab007066) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 18, 20, 22, 24 kDa Observed band size: 18,2 2, 25 kDa Exposure time: 8.0 s
Recombinant FGF2 Antibody (Ab007066) - IHC Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue sections labeling FGF2 with purified Recombinant FGF2 Antibody (Ab007066). Heat mediated antigen retrieval was performed.