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Recombinant Fibronectin Antibody - Primary antibody, specific to Fibronectin, Rabbit IgG

Recombinant

Validated

ExactAb™

0.25 mg/mL

Application:

Features and benefits

Host species: Rabbit
Species reactivity(Reacts with): Human
Isotype: Rabbit IgG
Conjugation: Unconjugated

Item Number

Ab103523

Grouped product items
SKU	Size	Availability	Price
Ab103523-10μl	10μl	In stock	$66.90
Ab103523-50μl	50μl	In stock	$199.90
Ab103523-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$309.90
Ab103523-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,809.90

Recombinant; Rabbit anti Human Fibronectin Antibody; WB, IHC; Unconjugated

View related series

Click to view target information: FN1

Accession#:P02751 Fibronectin FN1 Gene ID:2335

Basic Description

Product Name	Recombinant Fibronectin Antibody - Primary antibody, specific to Fibronectin, Rabbit IgG
Synonyms	CIG antibody \| Cold insoluble globulinantibody \| Cold-insoluble globulin antibody \| DKFZp686F10164antibody \| DKFZp686H0342 antibody \| DKFZp686I1370 antibody \| DKFZp686O13149 antibody \| ED Bantibody \| Fibronectin 1 antibody \| FINC antibody \| FINC_HUMAN ant
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.25 mg/mL
Host species	Rabbit
Specificity	Fibronectin
Immunogen	Recombinant protein of human Fibronectin (AA 1-2477).
Positive Control	WB: Hep G2 and HUVEC cell lysates. IHC: Human liver cancer tissue.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human Fibronectin Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	0.25 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Fibronectin Antibody (Ab103523) - Western Blot
All lanes: Recombinant Fibronectin Antibody (Ab103523) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 272 kDa
Observed band size: 270 kDa
Exposure time: 54.6 s

Recombinant Fibronectin Antibody (Ab103523) - IHC
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Recombinant Fibronectin Antibody (Ab103523). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

FN1 Tchem Fibronectin (0 Activities)

Discover Target: FN1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Application

Application	Dilution info
IHC	1/50 - 1/100 "> 1/50 - 1/100
WB	1/500-1/1000 "> 1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0303326	Certificate of Analysis	Mar 15, 2024	Ab103523
ZJ24F0303327	Certificate of Analysis	Mar 15, 2024	Ab103523

FAQs and Articles

FAQ

Immunohistochemical staining protocol for frozen samples (IHC-Fr)

Categories: Protocols

Tags:

IHC Monoclonal antibodies Immunohistochemistry Frozen section Recombinant Antibodies

Immunohistochemistry (IHC) staining antigen retrieval protocol

Categories: Protocols

Tags:

IHC Monoclonal antibodies Immunohistochemistry Recombinant Antibodies Antigen Retrieval

References

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20. Garcia-Pardo, A A, Pearlstein, E E and Frangione, B B.. (1985) Primary structure of human plasma fibronectin. Characterization of a 31,000-dalton fragment from the COOH-terminal region containing a free sulfhydryl group and a fibrin-binding site.. The Journal of biological chemistry, (25): [PMID:4019516]
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24. Garcia-Pardo, A A, Pearlstein, E E and Frangione, B B.. (1983) Primary structure of human plasma fibronectin. The 29,000-dalton NH2-terminal domain.. The Journal of biological chemistry, (25): [PMID:6630202]
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27. Potts, J R JR, Phan, I I, Williams, M J MJ and Campbell, I D ID.. (1995) High-resolution structural studies of the factor XIIIa crosslinking site and the first type 1 module of fibronectin.. Nature structural biology, [PMID:7583666]
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31. Leahy, D J DJ, Aukhil, I I and Erickson, H P HP.. (1996) 2.0 A crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region.. Cell, (12): [PMID:8548820]
32. Sasaki, T T, Brakebusch, C C, Engel, J J and Timpl, R R.. (1998) Mac-2 binding protein is a cell-adhesive protein of the extracellular matrix which self-assembles into ring-like structures and binds beta1 integrins, collagens and fibronectin.. The EMBO journal, (16): [PMID:9501082]
33. Sticht, H H, Pickford, A R AR, Potts, J R JR and Campbell, I D ID.. (1998) Solution structure of the glycosylated second type 2 module of fibronectin.. Journal of molecular biology, (13): [PMID:9514732]
34. Sharma, A A, Askari, J A JA, Humphries, M J MJ, Jones, E Y EY and Stuart, D I DI.. (1999) Crystal structure of a heparin- and integrin-binding segment of human fibronectin.. The EMBO journal, (15): [PMID:10075919]
35. Fattorusso, R R and 5 more authors.. (1999) NMR structure of the human oncofoetal fibronectin ED-B domain, a specific marker for angiogenesis.. Structure (London, England : 1993), (15): [PMID:10196121]
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37. Yi, M M and Ruoslahti, E E.. (2001) A fibronectin fragment inhibits tumor growth, angiogenesis, and metastasis.. Proceedings of the National Academy of Sciences of the United States of America, (16): [PMID:11209058]
38. Pickford, A R AR, Smith, S P SP, Staunton, D D, Boyd, J J and Campbell, I D ID.. (2001) The hairpin structure of the (6)F1(1)F2(2)F2 fragment from human fibronectin enhances gelatin binding.. The EMBO journal, (2): [PMID:11285216]
39. Holmes, A R AR and 6 more authors.. (2001) The pavA gene of Streptococcus pneumoniae encodes a fibronectin-binding protein that is essential for virulence.. Molecular microbiology, [PMID:11580843]
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42. Niimi, T T and 7 more authors.. (2001) NMR structure of human fibronectin EDA.. Journal of biomolecular NMR, [PMID:11775745]
43. Tu, Hongmin H and 6 more authors.. (2002) The type XIII collagen ectodomain is a 150-nm rod and capable of binding to fibronectin, nidogen-2, perlecan, and heparin.. The Journal of biological chemistry, (21): [PMID:11956183]
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53. Maguer-Satta, Véronique V and 6 more authors.. (2006) A novel role for fibronectin type I domain in the regulation of human hematopoietic cell adhesiveness through binding to follistatin domains of FLRG and follistatin.. Experimental cell research, (15): [PMID:16336961]
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56. Kuznetsova, Svetlana A SA and 9 more authors.. (2008) TSG-6 binds via its CUB_C domain to the cell-binding domain of fibronectin and increases fibronectin matrix assembly.. Matrix biology : journal of the International Society for Matrix Biology, [PMID:18042364]
57. Castelletti, Federica F and 14 more authors.. (2008) Mutations in FN1 cause glomerulopathy with fibronectin deposits.. Proceedings of the National Academy of Sciences of the United States of America, (19): [PMID:18268355]
58. Han, Guanghui G and 9 more authors.. (2008) Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography.. Proteomics, [PMID:18318008]
59. Bingham, Richard J RJ and 7 more authors.. (2008) Crystal structures of fibronectin-binding sites from Staphylococcus aureus FnBPA in complex with fibronectin domains.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:18713862]
60. Erat, Michèle C MC and 6 more authors.. (2009) Identification and structural analysis of type I collagen sites in complex with fibronectin fragments.. Proceedings of the National Academy of Sciences of the United States of America, (17): [PMID:19251642]
61. You, Ran; Klein, R Matthew; Zheng, Mingzhe and McKeown-Longo, Paula J.. (2009) Regulation of p38 MAP kinase by anastellin is independent of anastellin's effect on matrix fibronectin.. Matrix biology : journal of the International Society for Matrix Biology, [PMID:19379667]
62. Zhou, Tianjun T and 8 more authors.. (2010) Structural analysis of DFG-in and DFG-out dual Src-Abl inhibitors sharing a common vinyl purine template.. Chemical biology & drug design, [PMID:19895503]
63. Graille, Marc M, Pagano, Maurice M, Rose, Thierry T, Ravaux, Michèle Reboud MR and van Tilbeurgh, Herman H.. (2010) Zinc induces structural reorganization of gelatin binding domain from human fibronectin and affects collagen binding.. Structure (London, England : 1993), (9): [PMID:20541508]
64. Rosenow, Anja A and 6 more authors.. (2012) Resveratrol-induced changes of the human adipocyte secretion profile.. Journal of proteome research, (7): [PMID:22905912]
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67. Lee, Chae Syng CS and 33 more authors.. (2017) Mutations in Fibronectin Cause a Subtype of Spondylometaphyseal Dysplasia with "Corner Fractures".. American journal of human genetics, (2): [PMID:29100092]
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