Recombinant; Rabbit anti Human Fibronectin Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab103523-10μl | 10μl | In stock | $66.90 | |
| Ab103523-50μl | 50μl | In stock | $199.90 | |
| Ab103523-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab103523-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,809.90 |
Recombinant; Rabbit anti Human Fibronectin Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant Fibronectin Antibody - Primary antibody, specific to Fibronectin, Rabbit IgG |
|---|---|
| Synonyms | CIG antibody; Cold insoluble globulinantibody; Cold-insoluble globulin antibody; DKFZp686F10164antibody; DKFZp686H0342 antibody; DKFZp686I1370 antibody; DKFZp686O13149 antibody; ED Bantibody; Fibronectin 1 antibody; FINC antibody; FINC_HUMAN antibody; FN |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.25 mg/mL |
| Host species | Rabbit |
| Specificity | Fibronectin |
| Immunogen | Recombinant protein of human Fibronectin (AA 1-2477). |
| Positive Control | WB: Hep G2 and HUVEC cell lysates. IHC: Human liver cancer tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Fibronectin Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Fibronectin Antibody (Ab103523) - Western Blot
All lanes: Recombinant Fibronectin Antibody (Ab103523) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 272 kDa
Observed band size: 270 kDa
Exposure time: 54.6 s
Recombinant Fibronectin Antibody (Ab103523) - Western Blot
All lanes: Recombinant Fibronectin Antibody (Ab103523) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 272 kDa
Observed band size: 220, 260 kDa
Exposure time: 91.5 s
Recombinant Fibronectin Antibody (Ab103523) - IHC
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Recombinant Fibronectin Antibody (Ab103523). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| IHC | 1/50 - 1/100 |
| WB |
1/500-1/1000
|
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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303326 | Certificate of Analysis | Mar 15, 2024 | Ab103523 |
| ZJ24F0303327 | Certificate of Analysis | Mar 15, 2024 | Ab103523 |
| 1. Baron, M M and 5 more authors.. (1992) 1H NMR assignment and secondary structure of the cell adhesion type III module of fibronectin.. Biochemistry, (25): [PMID:1311202] |
| 2. Balbona, K K and 5 more authors.. (1992) Fibulin binds to itself and to the carboxyl-terminal heparin-binding region of fibronectin.. The Journal of biological chemistry, (5): [PMID:1400330] |
| 3. Main, A L AL, Harvey, T S TS, Baron, M M, Boyd, J J and Campbell, I D ID.. (1992) The three-dimensional structure of the tenth type III module of fibronectin: an insight into RGD-mediated interactions.. Cell, (13): [PMID:1423622] |
| 4. Tressel, T T and 6 more authors.. (1991) Human plasma fibronectin. Demonstration of structural differences between the A- and B-chains in the III CS region.. The Biochemical journal, (15): [PMID:2012601] |
| 5. Liu, M C MC, Yu, S S, Sy, J J, Redman, C M CM and Lipmann, F F.. (1985) Tyrosine sulfation of proteins from the human hepatoma cell line HepG2.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2414772] |
| 6. Zardi, L L and 6 more authors.. (1987) Transformed human cells produce a new fibronectin isoform by preferential alternative splicing of a previously unobserved exon.. The EMBO journal, [PMID:2822387] |
| 7. Umezawa, K K, Kornblihtt, A R AR and Baralle, F E FE.. (1985) Isolation and characterization of cDNA clones for human liver fibronectin.. FEBS letters, (1): [PMID:2989004] |
| 8. Bernard, M P MP, Kolbe, M M, Weil, D D and Chu, M L ML.. (1985) Human cellular fibronectin: comparison of the carboxyl-terminal portion with rat identifies primary structural domains separated by hypervariable regions.. Biochemistry, (21): [PMID:2992573] |
| 9. Kornblihtt, A R AR, Umezawa, K K, Vibe-Pedersen, K K and Baralle, F E FE.. (1985) Primary structure of human fibronectin: differential splicing may generate at least 10 polypeptides from a single gene.. The EMBO journal, [PMID:2992939] |
| 10. Oldberg, A A and Ruoslahti, E E.. (1986) Evolution of the fibronectin gene. Exon structure of cell attachment domain.. The Journal of biological chemistry, (15): [PMID:3003095] |
| 11. Sekiguchi, K K, Klos, A M AM, Kurachi, K K, Yoshitake, S S and Hakomori, S S.. (1986) Human liver fibronectin complementary DNAs: identification of two different messenger RNAs possibly encoding the alpha and beta subunits of plasma fibronectin.. Biochemistry, (26): [PMID:3021206] |
| 12. Owens, R J RJ and Baralle, F E FE.. (1986) Mapping the collagen-binding site of human fibronectin by expression in Escherichia coli.. The EMBO journal, [PMID:3024962] |
| 13. Dean, D C DC, Bowlus, C L CL and Bourgeois, S S.. (1987) Cloning and analysis of the promotor region of the human fibronectin gene.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3031656] |
| 14. Paolella, G G, Henchcliffe, C C, Sebastio, G G and Baralle, F E FE.. (1988) Sequence analysis and in vivo expression show that alternative splicing of ED-B and ED-A regions of the human fibronectin gene are independent events.. Nucleic acids research, (25): [PMID:3375063] |
| 15. Gutman, A A and Kornblihtt, A R AR.. (1987) Identification of a third region of cell-specific alternative splicing in human fibronectin mRNA.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3478690] |
| 16. Garcia-Pardo, A A, Rostagno, A A and Frangione, B B.. (1987) Primary structure of human plasma fibronectin. Characterization of a 38 kDa domain containing the C-terminal heparin-binding site (Hep III site) and a region of molecular heterogeneity.. The Biochemical journal, (1): [PMID:3593230] |
| 17. Gutman, A A, Yamada, K M KM and Kornblihtt, A A.. (1986) Human fibronectin is synthesized as a pre-propolypeptide.. FEBS letters, (20): [PMID:3770189] |
| 18. Vibe-Pedersen, K K, Magnusson, S S and Baralle, F E FE.. (1986) Donor and acceptor splice signals within an exon of the human fibronectin gene: a new type of differential splicing.. FEBS letters, (27): [PMID:3770201] |
| 19. Calaycay, J J and 6 more authors.. (1985) Primary structure of a DNA- and heparin-binding domain (Domain III) in human plasma fibronectin.. The Journal of biological chemistry, (5): [PMID:3900070] |
| 20. Garcia-Pardo, A A, Pearlstein, E E and Frangione, B B.. (1985) Primary structure of human plasma fibronectin. Characterization of a 31,000-dalton fragment from the COOH-terminal region containing a free sulfhydryl group and a fibrin-binding site.. The Journal of biological chemistry, (25): [PMID:4019516] |
| 21. Kornblihtt, A R AR, Vibe-Pedersen, K K and Baralle, F E FE.. (1984) Human fibronectin: molecular cloning evidence for two mRNA species differing by an internal segment coding for a structural domain.. The EMBO journal, [PMID:6200322] |
| 22. Kornblihtt, A R AR, Vibe-Pedersen, K K and Baralle, F E FE.. (1983) Isolation and characterization of cDNA clones for human and bovine fibronectins.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6304699] |
| 23. Kornblihtt, A R AR, Vibe-Pedersen, K K and Baralle, F E FE.. (1984) Human fibronectin: cell specific alternative mRNA splicing generates polypeptide chains differing in the number of internal repeats.. Nucleic acids research, (25): [PMID:6462919] |
| 24. Garcia-Pardo, A A, Pearlstein, E E and Frangione, B B.. (1983) Primary structure of human plasma fibronectin. The 29,000-dalton NH2-terminal domain.. The Journal of biological chemistry, (25): [PMID:6630202] |
| 25. Oldberg, A A, Linney, E E and Ruoslahti, E E.. (1983) Molecular cloning and nucleotide sequence of a cDNA clone coding for the cell attachment domain in human fibronectin.. The Journal of biological chemistry, (10): [PMID:6688418] |
| 26. Pierschbacher, M D MD, Ruoslahti, E E, Sundelin, J J, Lind, P P and Peterson, P A PA.. (1982) The cell attachment domain of fibronectin. Determination of the primary structure.. The Journal of biological chemistry, (25): [PMID:7050098] |
| 27. Potts, J R JR, Phan, I I, Williams, M J MJ and Campbell, I D ID.. (1995) High-resolution structural studies of the factor XIIIa crosslinking site and the first type 1 module of fibronectin.. Nature structural biology, [PMID:7583666] |
| 28. Rostagno, A A, Williams, M J MJ, Baron, M M, Campbell, I D ID and Gold, L I LI.. (1994) Further characterization of the NH2-terminal fibrin-binding site on fibronectin.. The Journal of biological chemistry, (16): [PMID:7989369] |
| 29. Dickinson, C D CD and 6 more authors.. (1994) Crystal structure of the tenth type III cell adhesion module of human fibronectin.. Journal of molecular biology, (4): [PMID:8120888] |
| 30. Williams, M J MJ and 5 more authors.. (1994) Solution structure of a pair of fibronectin type 1 modules with fibrin binding activity.. Journal of molecular biology, (28): [PMID:8308892] |
| 31. Leahy, D J DJ, Aukhil, I I and Erickson, H P HP.. (1996) 2.0 A crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region.. Cell, (12): [PMID:8548820] |
| 32. Sasaki, T T, Brakebusch, C C, Engel, J J and Timpl, R R.. (1998) Mac-2 binding protein is a cell-adhesive protein of the extracellular matrix which self-assembles into ring-like structures and binds beta1 integrins, collagens and fibronectin.. The EMBO journal, (16): [PMID:9501082] |
| 33. Sticht, H H, Pickford, A R AR, Potts, J R JR and Campbell, I D ID.. (1998) Solution structure of the glycosylated second type 2 module of fibronectin.. Journal of molecular biology, (13): [PMID:9514732] |
| 34. Sharma, A A, Askari, J A JA, Humphries, M J MJ, Jones, E Y EY and Stuart, D I DI.. (1999) Crystal structure of a heparin- and integrin-binding segment of human fibronectin.. The EMBO journal, (15): [PMID:10075919] |
| 35. Fattorusso, R R and 5 more authors.. (1999) NMR structure of the human oncofoetal fibronectin ED-B domain, a specific marker for angiogenesis.. Structure (London, England : 1993), (15): [PMID:10196121] |
| 36. Bocquier, A A AA, Potts, J R JR, Pickford, A R AR and Campbell, I D ID.. (1999) Solution structure of a pair of modules from the gelatin-binding domain of fibronectin.. Structure (London, England : 1993), (15): [PMID:10647176] |
| 37. Yi, M M and Ruoslahti, E E.. (2001) A fibronectin fragment inhibits tumor growth, angiogenesis, and metastasis.. Proceedings of the National Academy of Sciences of the United States of America, (16): [PMID:11209058] |
| 38. Pickford, A R AR, Smith, S P SP, Staunton, D D, Boyd, J J and Campbell, I D ID.. (2001) The hairpin structure of the (6)F1(1)F2(2)F2 fragment from human fibronectin enhances gelatin binding.. The EMBO journal, (2): [PMID:11285216] |
| 39. Holmes, A R AR and 6 more authors.. (2001) The pavA gene of Streptococcus pneumoniae encodes a fibronectin-binding protein that is essential for virulence.. Molecular microbiology, [PMID:11580843] |
| 40. Schor, S L SL and Schor, A M AM.. (2001) Phenotypic and genetic alterations in mammary stroma: implications for tumour progression.. Breast cancer research : BCR, [PMID:11737888] |
| 41. Filla, Mark S MS and 6 more authors.. (2002) In vitro localization of TIGR/MYOC in trabecular meshwork extracellular matrix and binding to fibronectin.. Investigative ophthalmology & visual science, [PMID:11773026] |
| 42. Niimi, T T and 7 more authors.. (2001) NMR structure of human fibronectin EDA.. Journal of biomolecular NMR, [PMID:11775745] |
| 43. Tu, Hongmin H and 6 more authors.. (2002) The type XIII collagen ectodomain is a 150-nm rod and capable of binding to fibronectin, nidogen-2, perlecan, and heparin.. The Journal of biological chemistry, (21): [PMID:11956183] |
| 44. Parker, A E AE, Boutell, J J, Carr, A A and Maciewicz, R A RA.. (2002) Novel cartilage-specific splice variants of fibronectin.. Osteoarthritis and cartilage, [PMID:12127832] |
| 45. Di Cesare, Paul E PE and 6 more authors.. (2002) Matrix-matrix interaction of cartilage oligomeric matrix protein and fibronectin.. Matrix biology : journal of the International Society for Matrix Biology, [PMID:12225811] |
| 46. Schwarz-Linek, Ulrich U and 10 more authors.. (2003) Pathogenic bacteria attach to human fibronectin through a tandem beta-zipper.. Nature, (8): [PMID:12736686] |
| 47. Gao, Mu M and 5 more authors.. (2003) Structure and functional significance of mechanically unfolded fibronectin type III1 intermediates.. Proceedings of the National Academy of Sciences of the United States of America, (9): [PMID:14657397] |
| 48. Bunkenborg, Jakob J, Pilch, Bartosz J BJ, Podtelejnikov, Alexandre V AV and Wiśniewski, Jacek R JR.. (2004) Screening for N-glycosylated proteins by liquid chromatography mass spectrometry.. Proteomics, [PMID:14760718] |
| 49. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 50. Kato, Seishi S, Ohtoko, Kuniyo K, Ohtake, Hideki H and Kimura, Tomoko T.. (2005) Vector-capping: a simple method for preparing a high-quality full-length cDNA library.. DNA research : an international journal for rapid publication of reports on genes and genomes, (28): [PMID:16106752] |
| 51. Kay, Richard A RA and 6 more authors.. (2005) The expression of migration stimulating factor, a potent oncofetal cytokine, is uniquely controlled by 3'-untranslated region-dependent nuclear sequestration of its precursor messenger RNA.. Cancer research, (1): [PMID:16322219] |
| 52. Liu, Tao T and 6 more authors.. () Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952] |
| 53. Maguer-Satta, Véronique V and 6 more authors.. (2006) A novel role for fibronectin type I domain in the regulation of human hematopoietic cell adhesiveness through binding to follistatin domains of FLRG and follistatin.. Experimental cell research, (15): [PMID:16336961] |
| 54. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974] |
| 55. Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31): [PMID:17974005] |
| 56. Kuznetsova, Svetlana A SA and 9 more authors.. (2008) TSG-6 binds via its CUB_C domain to the cell-binding domain of fibronectin and increases fibronectin matrix assembly.. Matrix biology : journal of the International Society for Matrix Biology, [PMID:18042364] |
| 57. Castelletti, Federica F and 14 more authors.. (2008) Mutations in FN1 cause glomerulopathy with fibronectin deposits.. Proceedings of the National Academy of Sciences of the United States of America, (19): [PMID:18268355] |
| 58. Han, Guanghui G and 9 more authors.. (2008) Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography.. Proteomics, [PMID:18318008] |
| 59. Bingham, Richard J RJ and 7 more authors.. (2008) Crystal structures of fibronectin-binding sites from Staphylococcus aureus FnBPA in complex with fibronectin domains.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:18713862] |
| 60. Erat, Michèle C MC and 6 more authors.. (2009) Identification and structural analysis of type I collagen sites in complex with fibronectin fragments.. Proceedings of the National Academy of Sciences of the United States of America, (17): [PMID:19251642] |
| 61. You, Ran; Klein, R Matthew; Zheng, Mingzhe and McKeown-Longo, Paula J.. (2009) Regulation of p38 MAP kinase by anastellin is independent of anastellin's effect on matrix fibronectin.. Matrix biology : journal of the International Society for Matrix Biology, [PMID:19379667] |
| 62. Zhou, Tianjun T and 8 more authors.. (2010) Structural analysis of DFG-in and DFG-out dual Src-Abl inhibitors sharing a common vinyl purine template.. Chemical biology & drug design, [PMID:19895503] |
| 63. Graille, Marc M, Pagano, Maurice M, Rose, Thierry T, Ravaux, Michèle Reboud MR and van Tilbeurgh, Herman H.. (2010) Zinc induces structural reorganization of gelatin binding domain from human fibronectin and affects collagen binding.. Structure (London, England : 1993), (9): [PMID:20541508] |
| 64. Rosenow, Anja A and 6 more authors.. (2012) Resveratrol-induced changes of the human adipocyte secretion profile.. Journal of proteome research, (7): [PMID:22905912] |
| 65. Tsui, Irena I, Pan, Carolyn K CK, Rahimy, Ehsan E and Schwartz, Steven D SD.. (2012) Ocriplasmin for vitreoretinal diseases.. Journal of biomedicine & biotechnology, [PMID:23193358] |
| 66. Tagliabracci, Vincent S VS and 14 more authors.. (2015) A Single Kinase Generates the Majority of the Secreted Phosphoproteome.. Cell, (18): [PMID:26091039] |
| 67. Lee, Chae Syng CS and 33 more authors.. (2017) Mutations in Fibronectin Cause a Subtype of Spondylometaphyseal Dysplasia with "Corner Fractures".. American journal of human genetics, (2): [PMID:29100092] |
| 68. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
