Recombinant FMRP Antibody - Primary antibody, specific to FMRP, Rabbit IgG
Synonyms
FMR 1 antibody | Fmr1 antibody | Fmr1 gene antibody | FMR1_HUMAN antibody | FMRP antibody | Fragile X mental retardation 1 antibody | Fragile X mental retardation 1 protein antibody | Fragile X mental retardation protein 1 antibody | Fragile X mental reta
Specifications & Purity
ExactAb™, Validated, Recombinant, 1.0 mg/mL
Host species
Rabbit
Specificity
FMRP
Immunogen
A synthetic peptide derived from human FMRP (AA 31-47)
Positive Control
WB: HEK293, U-2 OS, Jurkat, K562, HeLa, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human FMRP Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant FMRP Antibody (Ab103843) - Western Blot All lanes: Recombinant FMRP Antibody (Ab103843) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 71 kDa Observed band size: 64-80 kDa Exposure time: 22.8 s
Recombinant FMRP Antibody (Ab103843) - IF/ICC Immunocytochemistry analysis of FMRP (green) in HeLa using Recombinant FMRP Antibody (Ab103843), and cells were counterstained with DAPI (blue).
Recombinant FMRP Antibody (Ab103843) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant FMRP Antibody (Ab103843). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.