Recombinant FOXA2 Antibody - Primary antibody, specific to FOXA2, Rabbit IgG

Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human FOXA2 Antibody; WB; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse
  • Isotype: Rabbit IgG
    Application:
  • WB
Item Number
Ab103920
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab103920-10μl
10μl
In stock
$66.90
Ab103920-50μl
50μl
In stock
$199.90
Ab103920-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$329.90
Ab103920-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

Recombinant; Rabbit anti Human FOXA2 Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant FOXA2 Antibody - Primary antibody, specific to FOXA2, Rabbit IgG
SynonymsForkhead box A2 antibody; Forkhead box protein A2 antibody; FOX A2 antibody; foxa2 antibody; FOXA2_HUMAN antibody; Hepatic nuclear factor 3 beta antibody; Hepatocyte nuclear factor 3 antibody; Hepatocyte nuclear factor 3 beta antibody; Hepatocyte nuclear
Specifications & PurityExactAb™, Validated, Recombinant, 1.5 mg/mL
Host speciesRabbit
SpecificityFOXA2
ImmunogenA synthetic peptide derived from human FOXA2 (AA 1-53)
Positive ControlWB: Mouse liver and Hep G2 lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human FOXA2 Antibody, Recombinant, could be used for WB and so on.
Application
WB: 1/500 – 1/2000
Protein Function
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration1.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant FOXA2 Antibody (Ab103920) - Western Blot
All lanes: Recombinant FOXA2 Antibody (Ab103920) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 50, 60 kDa
Exposure time: 10.1 s

Associated Targets(Human)

FOXA2 Tbio Hepatocyte nuclear factor 3-beta (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/2000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0202828Certificate of AnalysisFeb 23, 2024 Ab103920
ZJ24F0202829Certificate of AnalysisFeb 23, 2024 Ab103920

Related Documents

References

1. Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J.  (2001)  The DNA sequence and comparative analysis of human chromosome 20..  Nature,  414  (6866): (865-71).  [PMID:11780052]
2. Yamada, S S and 15 more authors..  (2000)  Cloning of cDNA and the gene encoding human hepatocyte nuclear factor (HNF)-3 beta and mutation screening in Japanese subjects with maturity-onset diabetes of the young..  Diabetologia,      [PMID:10672453]
3. Navas, M A MA and 5 more authors..  ()  The human HNF-3 genes: cloning, partial sequence and mutation screening in patients with impaired glucose homeostasis..  Human heredity,      [PMID:10899756]
4. Wolfrum, Christian C, Besser, Daniel D, Luca, Edlira E and Stoffel, Markus M..  (2003)  Insulin regulates the activity of forkhead transcription factor Hnf-3beta/Foxa-2 by Akt-mediated phosphorylation and nuclear/cytosolic localization..  Proceedings of the National Academy of Sciences of the United States of America,    (30):   [PMID:14500912]
5. Verschuur, Maartje M, de Jong, Maureen M, Felida, Lamberto L, de Maat, Moniek P M MP and Vos, Hans L HL..  (2005)  A hepatocyte nuclear factor-3 site in the fibrinogen beta promoter is important for interleukin 6-induced expression, and its activity is influenced by the adjacent -148C/T polymorphism..  The Journal of biological chemistry,    (29):   [PMID:15737987]
6. Motallebipour, Mehdi M and 9 more authors..  (2009)  Differential binding and co-binding pattern of FOXA1 and FOXA3 and their relation to H3K4me3 in HepG2 cells revealed by ChIP-seq..  Genome biology,      [PMID:19919681]

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