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Recombinant Frizzled 8 Antibody - Primary antibody, specific to FZD8, Rabbit IgG

Recombinant

Validated

ExactAb™

1.0 mg/mL

Application:

Features and benefits

Host species: Rabbit
Species reactivity(Reacts with): Human,Mouse,Rat
Isotype: Rabbit IgG
Conjugation: Unconjugated

Item Number

Ab104134

Grouped product items
SKU	Size	Availability	Price
Ab104134-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$76.90
Ab104134-50μl	50μl	In stock	$229.90
Ab104134-100μl	100μl	In stock	$329.90
Ab104134-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,699.90

Recombinant; Rabbit anti Human Frizzled 8 Antibody; WB; Unconjugated

View related series

Click to view target information: FZD8

Accession#:Q9H461 Frizzled 8 FZD8 Gene ID:8325

Basic Description

Product Name	Recombinant Frizzled 8 Antibody - Primary antibody, specific to FZD8, Rabbit IgG
Synonyms	Frizzled-8 \| FZ8 \| FZD8 \| hFz8 \| Frizzled 8 seven transmembrane spanning receptor antibody \| frizzled 8, seven transmembrane spanning receptor antibody \| Frizzled family receptor 8 antibody \| frizzled homolog 8 (Drosophila) antibody \| Frizzled homolog 8 a
Specifications & Purity	ExactAb™, Validated, Recombinant, 1.0 mg/mL
Host species	Rabbit
Specificity	FZD8
Immunogen	A synthetic peptide derived from human Frizzled 8 (AA 640-694).
Epitope	HeLa and Jurkat lysates; Human endometrial carcinoma tissue.
Positive Control	WB：Jurkat lysates
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human Frizzled 8 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Frizzled 8 Antibody (Ab104134) - Western Blot
All lanes: Recombinant Frizzled 8 Antibody (Ab104134) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 73 kDa
Observed band size: 66,42 kDa
Exposure time: 5.6 s

Associated Targets(Human)

FZD8 Tbio Frizzled-8 (0 Activities)

Discover Target: FZD8

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

FZD8 Tbio Frizzled-8 (3 Activities)

Discover Target: FZD8

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Application

Dilution info

1/500-1/1000

Certificates

Certificate of Analysis(COA)

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Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F1101533	Certificate of Analysis	Nov 02, 2023	Ab104134
ZJ23F1101534	Certificate of Analysis	Nov 02, 2023	Ab104134

References

1. Saitoh T, Hirai M, Katoh M. (2001) Molecular cloning and characterization of human Frizzled-8 gene on chromosome 10p11.2.. Int J Oncol, 18 (5): (991-6). [PMID:11295046]
2. Zhao Y, Ren J, Hillier J, Lu W, Jones EY. (2020) Antiepileptic Drug Carbamazepine Binds to a Novel Pocket on the Wnt Receptor Frizzled-8.. J Med Chem, 63 (6): (3252-3260). [PMID:32049522]
3. Semënov, M V MV and 5 more authors.. (2001) Head inducer Dickkopf-1 is a ligand for Wnt coreceptor LRP6.. Current biology : CB, (26): [PMID:11448771]
4. Deloukas, P P and 135 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 10.. Nature, (27): [PMID:15164054]
5. Li, Xiang X, Zhang, Jiahai J, Cao, Zanxia Z, Wu, Jihui J and Shi, Yunyu Y.. (2006) Solution structure of GOPC PDZ domain and its interaction with the C-terminal motif of neuroligin.. Protein science : a publication of the Protein Society, [PMID:16882988]
6. Hao, Huai-Xiang HX and 18 more authors.. (2012) ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner.. Nature, (29): [PMID:22575959]
7. Capurro, Mariana M, Martin, Tonya T, Shi, Wen W and Filmus, Jorge J.. (2014) Glypican-3 binds to Frizzled and plays a direct role in the stimulation of canonical Wnt signaling.. Journal of cell science, (1): [PMID:24496449]

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