Recombinant; Rabbit anti Human GAD67 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab104489-10μl | 10μl | In stock | $59.90 | |
| Ab104489-50μl | 50μl | In stock | $179.90 | |
| Ab104489-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab104489-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,399.90 |
Recombinant; Rabbit anti Human GAD67 Antibody; WB; Unconjugated
| Product Name | Recombinant GAD67 Antibody - Primary antibody, specific to GAD67, Rabbit IgG |
|---|---|
| Synonyms | 67 kDa glutamic acid decarboxylase antibody; CPSQ1 antibody; DCE1 antibody; DCE1_HUMAN antibody; EC 4.1.1.15 antibody; FLJ45882 antibody; GAD 1 antibody; GAD 67 antibody; GAD antibody; GAD-67 antibody; GAD1 antibody; Glutamate decarboxylase 1 (brain, 67kD |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.4 mg/mL |
| Host species | Rabbit |
| Specificity | GAD67 |
| Immunogen | A synthetic peptide derived from human GAD67 (AA 80-93) |
| Positive Control | WB: HeLa, HEK293, MCF7, Jurkat, K562, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human GAD67 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant GAD67 Antibody (Ab104489) - Western Blot
All lanes: Recombinant GAD67 Antibody (Ab104489) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 4.3 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
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| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303041 | Certificate of Analysis | Mar 08, 2024 | Ab104489 |
| ZJ23F1202159 | Certificate of Analysis | Dec 20, 2023 | Ab104489 |
| ZJ23F1202160 | Certificate of Analysis | Dec 20, 2023 | Ab104489 |
| 1. Kelly, C D CD and 7 more authors.. (1992) Nucleotide sequence and chromosomal assignment of a cDNA encoding the large isoform of human glutamate decarboxylase.. Annals of human genetics, [PMID:1339255] |
| 2. Bu, D F DF and 7 more authors.. (1992) Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:1549570] |
| 3. Persson, H H and 7 more authors.. (1990) Expression of the neurotransmitter-synthesizing enzyme glutamic acid decarboxylase in male germ cells.. Molecular and cellular biology, [PMID:1697032] |
| 4. Cram, D S DS, Barnett, L D LD, Joseph, J L JL and Harrison, L C LC.. (1991) Cloning and partial nucleotide sequence of human glutamic acid decarboxylase cDNA from brain and pancreatic islets.. Biochemical and biophysical research communications, (15): [PMID:2039509] |
| 5. Bu, D F DF and Tobin, A J AJ.. (1994) The exon-intron organization of the genes (GAD1 and GAD2) encoding two human glutamate decarboxylases (GAD67 and GAD65) suggests that they derive from a common ancestral GAD.. Genomics, (1): [PMID:8088791] |
| 6. Yamashita, K K, Cram, D S DS and Harrison, L C LC.. (1993) Molecular cloning of full-length glutamic acid decarboxylase 67 from human pancreas and islets.. Biochemical and biophysical research communications, (14): [PMID:8507202] |
| 7. Kawasaki, E E and 9 more authors.. (1993) Cloning and expression of large isoform of glutamic acid decarboxylase from human pancreatic islet.. Biochemical and biophysical research communications, (14): [PMID:8507203] |
| 8. Chessler, S D SD and Lernmark, A A.. (2000) Alternative splicing of GAD67 results in the synthesis of a third form of glutamic-acid decarboxylase in human islets and other non-neural tissues.. The Journal of biological chemistry, (18): [PMID:10671565] |
| 9. Tong, Jonathan C JC and 5 more authors.. (2002) Enzymatic characterization of a recombinant isoform hybrid of glutamic acid decarboxylase (rGAD67/65) expressed in yeast.. Journal of biotechnology, (7): [PMID:12067524] |
| 10. Hwang, I K IK and 9 more authors.. (2004) Changes of pyridoxal kinase expression and activity in the gerbil hippocampus following transient forebrain ischemia.. Neuroscience, [PMID:15381280] |
| 11. Lynex, Clare N CN and 8 more authors.. (2004) Homozygosity for a missense mutation in the 67 kDa isoform of glutamate decarboxylase in a family with autosomal recessive spastic cerebral palsy: parallels with Stiff-Person Syndrome and other movement disorders.. BMC neurology, (30): [PMID:15571623] |
| 12. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 13. Fenalti, Gustavo G and 18 more authors.. (2007) GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop.. Nature structural & molecular biology, [PMID:17384644] |
| 14. Tronci, Elisabetta E and 6 more authors.. (2007) Characterization of the antiparkinsonian effects of the new adenosine A2A receptor antagonist ST1535: acute and subchronic studies in rats.. European journal of pharmacology, (2): [PMID:17445798] |
| 15. Langendorf, Christopher G and 9 more authors.. (2013) Structural characterization of the mechanism through which human glutamic acid decarboxylase auto-activates.. Bioscience reports, (11): [PMID:23126365] |
| 16. Chatron, Nicolas and 30 more authors.. (2020) Bi-allelic GAD1 variants cause a neonatal onset syndromic developmental and epileptic encephalopathy.. Brain : a journal of neurology, (1): [PMID:32282878] |
| 17. Morgan, Neil V and 9 more authors.. (2021) Evidence that autosomal recessive spastic cerebral palsy-1 (CPSQ1) is caused by a missense variant in HPDL.. Brain communications, [PMID:33634263] |
