Recombinant (RR688); Rabbit anti Human GAPDH Antibody; WB, IHC, Flow, IF, ICC, IP; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab104788-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $49.90 | |
| Ab104788-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $147.90 | |
| Ab104788-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $237.90 | |
| Ab104788-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,707.90 |
Recombinant (RR688); Rabbit anti Human GAPDH Antibody; WB, IHC, Flow, IF, ICC, IP; Unconjugated
| Product Name | Recombinant GAPDH Antibody - Primary antibody, specific to GAPDH, Rabbit IgG |
|---|---|
| Synonyms | 38 kDa BFA-dependent ADP-ribosylation substrate antibody; aging associated gene 9 protein antibody; Aging-associated gene 9 protein antibody; BARS-38 antibody; cb609 antibody; EC 1.2.1.12 antibody; Epididymis secretory sperm binding protein Li 162eP antib |
| Specifications & Purity | ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | GAPDH |
| Immunogen | A synthetic peptide corresponding to residues of human GAPDH was used as an immunogen (AA 200-300). |
| Positive Control | WB: A431, MCF-7, A549, HEK293, HeLa, Raji, Jurkat, HUVEC, NIH/3T3 and C6 cell lysates. IHC: Prostate cancer tissue. Flow: HeLa cells. IF/ICC: HeLa cells. IP: HeLa whole cell lysate. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Recombinant, Validated |
| Product Description | Rabbit anti Human GAPDH Antibody, Recombinant (RR688), could be used for WB, IHC, Flow, IF, ICC, IP and so on. Application: WB: 1/5000 - 1/40000 IHC: 1/1600 - 1/3200 Flow: 1/200 - 1/1000 IF/ICC: 1/2000 - 1/10000 IP: 1/50 Protein Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Post-translational S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus. ISGylated. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant GAPDH Antibody (Ab104788) - Western Blot
All lanes: Recombinant GAPDH Antibody (Ab104788) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 1.0 s
Recombinant GAPDH Antibody (Ab104788) - IF
IF analysis of GAPDH (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant GAPDH Antibody (Ab104788) at 1/100 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant GAPDH Antibody (Ab104788) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of prostate cancer tissue labelling GAPDH with Recombinant GAPDH Antibody (Ab104788) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant GAPDH Antibody (Ab104788) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant GAPDH Antibody (Ab104788) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant GAPDH Antibody (Ab104788) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
Recombinant GAPDH Antibody (Ab104788) - IP
GAPDH was immunoprecipitated from 0.1 mg of HeLa whole cell lysate with Recombinant GAPDH Antibody (Ab104788) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant GAPDH Antibody (Ab104788) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant GAPDH Antibody (Ab104788) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 2 μg (input).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IP |
1/50
|
| Flow |
1/200 - 1/1000
|
| IF/ICC | 1/2000 - 1/10000 |
| WB | 1/5000 - 1/40000 |
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| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505915 | Certificate of Analysis | May 21, 2024 | Ab104788 |
| ZJ23F0901096 | Certificate of Analysis | Sep 18, 2023 | Ab104788 |
| ZJ23F0901097 | Certificate of Analysis | Sep 18, 2023 | Ab104788 |
