A synthetic peptide derived from human GATA3 (AA 1-220).
Positive Control
WB: Jurkat, HEK293 and K562 cell lysates. IHC: Human bladder cancer tissues.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human GATA3 Antibody, Recombinant, could be used for WB and IHC. Application: WB: 1/500 - 1/1000 IHC: 1/50 - 1/100 Protein Function Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
Product Properties
Antibody Type
Primary antibody
Clonality
Recombinant
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Source
Cell supernatant
Form
Liquid
Concentration
1.2mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant GATA3 Antibody (Ab104881) - Western Blot All lanes: Recombinant GATA3 Antibody (Ab104881) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 48 kDa Observed band size: 48&37 kDa Specific bands were detected for GATA3 full length at approximately 48kDa and the splice form at approximately 37kDa. Exposure time: 2.3 s
Recombinant GATA3 Antibody (Ab104881) - Western Blot All lanes: Recombinant GATA3 Antibody (Ab104881) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 48 kDa Observed band size: 48&37 kDa Specific bands were detected for GATA3 full length at approximately 48kDa and the splice form at approximately 37kDa. Exposure time: 4.9 s
Recombinant GATA3 Antibody (Ab104881) - IHC Immunohistochemistry analysis of paraffin-embedded Human bladder cancer using Recombinant GATA3 Antibody (Ab104881)(1/50). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
ZJ23F0800802