| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab106394-10μl | 10μl | In stock | $69.90 | |
| Ab106394-50μl | 50μl | In stock | $199.90 | |
| Ab106394-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab106394-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,799.90 |
Recombinant; Rabbit anti Human GRB7 Antibody; WB; Unconjugated
| Product Name | Recombinant GRB7 Antibody - Primary antibody, specific to GRB7, Rabbit IgG | |
|---|---|---|
| Synonyms | B47 | Epidermal growth factor receptor GRB-7 | GRB7 adapter protein | B47 | Epidermal growth factor receptor GRB-7 | GRB7 adapter protein | GRB7 | growth factor receptor-bound protein 7 | B47 antibody | Epidermal growth factor receptor GRB 7 antibody | Ep | |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.6 mg/mL | |
| Host species | Rabbit | |
| Specificity | GRB7 | |
| Immunogen | A synthetic peptide derived from human GRB7 (AA 1-100) | |
| Positive Control | WB: A431, HT-29 and Hep G2 ccell lysates. | |
| Conjugation | Unconjugated | |
| Grade | ExactAb™, Recombinant, Validated | |
| Product Description |
|
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Anti-GRB7 antibody (Ab106394) - Western Blot
All lanes: Anti-GRB7 antibody (Ab106394) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 17.9 s
Anti-GRB7 antibody (Ab106394) - Western Blot
All lanes: Anti-GRB7 antibody (Ab106394) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 3.1 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0405010 | Certificate of Analysis | Apr 29, 2024 | Ab106394 |
| ZJ24F0405009 | Certificate of Analysis | Apr 29, 2024 | Ab106394 |
| 1. Ambaye ND, Gunzburg MJ, Lim RC, Price JT, Wilce MC, Wilce JA.. (2011) Benzopyrazine derivatives: A novel class of growth factor receptor bound protein 7 antagonists.. Bioorg Med Chem, 19 (1): (693-701). [PMID:21134757] [10.1016/j.bmc.2010.10.030] |
| 2. Watson GM, Gunzburg MJ, Ambaye ND, Lucas WA, Traore DA, Kulkarni K, Cergol KM, Payne RJ, Panjikar S, Pero SC, Perlmutter P, Wilce MC, Wilce JA.. (2015) Cyclic Peptides Incorporating Phosphotyrosine Mimetics as Potent and Specific Inhibitors of the Grb7 Breast Cancer Target.. J Med Chem, 58 (19): (7707-7718). [PMID:26359549] [10.1021/acs.jmedchem.5b00609] |
| 3. Tanaka, S S and 6 more authors.. (1997) Coexpression of Grb7 with epidermal growth factor receptor or Her2/erbB2 in human advanced esophageal carcinoma.. Cancer research, (1): [PMID:8988034] |
| 4. Kishi, T T and 7 more authors.. (1997) Molecular cloning of human GRB-7 co-amplified with CAB1 and c-ERBB-2 in primary gastric cancer.. Biochemical and biophysical research communications, (6): [PMID:9125150] |
| 5. Fiddes, R J RJ and 6 more authors.. (1998) Analysis of Grb7 recruitment by heregulin-activated erbB receptors reveals a novel target selectivity for erbB3.. The Journal of biological chemistry, (27): [PMID:9516479] |
| 6. Tanaka, S S and 6 more authors.. (1998) A novel variant of human Grb7 is associated with invasive esophageal carcinoma.. The Journal of clinical investigation, (15): [PMID:9710451] |
| 7. Vayssière, B B and 7 more authors.. (2000) Interaction of the Grb7 adapter protein with Rnd1, a new member of the Rho family.. FEBS letters, (4): [PMID:10664463] |
| 8. Kasus-Jacobi, A A, Béréziat, V V, Perdereau, D D, Girard, J J and Burnol, A F AF.. (2000) Evidence for an interaction between the insulin receptor and Grb7. A role for two of its binding domains, PIR and SH2.. Oncogene, (13): [PMID:10803466] |
| 9. Han, D C DC, Shen, T L TL and Guan, J L JL.. (2000) Role of Grb7 targeting to focal contacts and its phosphorylation by focal adhesion kinase in regulation of cell migration.. The Journal of biological chemistry, (15): [PMID:10893408] |
| 10. Shen, Tang-Long TL, Han, Dong Cho DC and Guan, Jun-Lin JL.. (2002) Association of Grb7 with phosphoinositides and its role in the regulation of cell migration.. The Journal of biological chemistry, (9): [PMID:12021278] |
| 11. Han, Dong Cho DC, Shen, Tang-Long TL, Miao, Hui H, Wang, Bingcheng B and Guan, Jun-Lin JL.. (2002) EphB1 associates with Grb7 and regulates cell migration.. The Journal of biological chemistry, (22): [PMID:12223469] |
| 12. Ivancic, Monika M, Daly, Roger J RJ and Lyons, Barbara A BA.. (2003) Solution structure of the human Grb7-SH2 domain/erbB2 peptide complex and structural basis for Grb7 binding to ErbB2.. Journal of biomolecular NMR, [PMID:12975581] |
| 13. Rönnstrand, L L.. (2004) Signal transduction via the stem cell factor receptor/c-Kit.. Cellular and molecular life sciences : CMLS, [PMID:15526160] |
| 14. Porter, Corrine J CJ, Wilce, Matthew C J MC, Mackay, Joel P JP, Leedman, Peter P and Wilce, Jackie A JA.. (2005) Grb7-SH2 domain dimerisation is affected by a single point mutation.. European biophysics journal : EBJ, [PMID:15841400] |
| 15. Roskoski, Robert R.. (2005) Signaling by Kit protein-tyrosine kinase--the stem cell factor receptor.. Biochemical and biophysical research communications, (11): [PMID:16129412] |
| 16. Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20): [PMID:16625196] |
| 17. Porter, Corrine J CJ and 9 more authors.. (2007) Grb7 SH2 domain structure and interactions with a cyclic peptide inhibitor of cancer cell migration and proliferation.. BMC structural biology, (25): [PMID:17894853] |
| 18. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 19. Siamakpour-Reihani, Sharareh S and 7 more authors.. () The cell migration protein Grb7 associates with transcriptional regulator FHL2 in a Grb7 phosphorylation-dependent manner.. Journal of molecular recognition : JMR, [PMID:18853468] |
| 20. Chu, Pei-Yu PY and 6 more authors.. (2009) Tyrosine phosphorylation of growth factor receptor-bound protein-7 by focal adhesion kinase in the regulation of cell migration, proliferation, and tumorigenesis.. The Journal of biological chemistry, (24): [PMID:19473962] |
| 21. Chu, Pei-Yu PY, Li, Tsai-Kun TK, Ding, Shih-Torng ST, Lai, I-Rue IR and Shen, Tang-Long TL.. (2010) EGF-induced Grb7 recruits and promotes Ras activity essential for the tumorigenicity of Sk-Br3 breast cancer cells.. The Journal of biological chemistry, (17): [PMID:20622016] |
| 22. Siamakpour-Reihani, Sharareh S and 11 more authors.. () Grb7 binds to Hax-1 and undergoes an intramolecular domain association that offers a model for Grb7 regulation.. Journal of molecular recognition : JMR, [PMID:20665473] |
