Recombinant; Rabbit anti Human HCE Antibody; WB, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab106948-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $89.90 | |
| Ab106948-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $269.90 | |
| Ab106948-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $379.90 | |
| Ab106948-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Recombinant; Rabbit anti Human HCE Antibody; WB, IF, ICC; Unconjugated
| Product Name | Recombinant HCE Antibody - Primary antibody, specific to RNGTT, Rabbit IgG |
|---|---|
| Synonyms | HCAP1 | HCE | RNGTT | CAP1A | GTase | GTP--RNA guanylyltransferase | hCAP | HCAP1 | HcE | HCE1 | MCE1 | MCE1_HUMAN | mRNA 5''-triphosphatase | mRNA capping enzyme | mRNA guanyltransferase | mRNA guanylyltransferase | polynucleotide 5' trisphosphatase | RN |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.8 mg/mL |
| Host species | Rabbit |
| Specificity | RNGTT |
| Immunogen | Recombinant protein of human HCE (AA 1-212) |
| Positive Control | WB: HeLa, A549, U-2 OS and C6 cell lysates. ICC/IF: MCF7 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human HCE Antibody, Recombinant, could be used for WB, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant HCE Antibody (Ab106948) - Western Blot
All lanes: Recombinant HCE Antibody (Ab106948) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 70 kDa
Exposure time: 2.3 s
Recombinant HCE Antibody (Ab106948) - IF/ICC
Immunocytochemistry analysis of HCE (green) in MCF7 using Recombinant HCE Antibody (Ab106948) and DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0607052 | Certificate of Analysis | Jun 19, 2024 | Ab106948 |
| ZJ24F0607051 | Certificate of Analysis | Jun 19, 2024 | Ab106948 |
| 1. Yue, Z Z and 5 more authors.. (1997) Mammalian capping enzyme complements mutant Saccharomyces cerevisiae lacking mRNA guanylyltransferase and selectively binds the elongating form of RNA polymerase II.. Proceedings of the National Academy of Sciences of the United States of America, (25): [PMID:9371772] |
| 2. Tsukamoto, T T and 6 more authors.. (1998) Cloning and characterization of two human cDNAs encoding the mRNA capping enzyme.. Biochemical and biophysical research communications, (4): [PMID:9473487] |
| 3. Yamada-Okabe, T T, Doi, R R, Shimmi, O O, Arisawa, M M and Yamada-Okabe, H H.. (1998) Isolation and characterization of a human cDNA for mRNA 5'-capping enzyme.. Nucleic acids research, (1): [PMID:9512541] |
| 4. Pillutla, R C RC, Yue, Z Z, Maldonado, E E and Shatkin, A J AJ.. (1998) Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes.. The Journal of biological chemistry, (21): [PMID:9705270] |
| 5. Wen, Y Y and Shatkin, A J AJ.. (1999) Transcription elongation factor hSPT5 stimulates mRNA capping.. Genes & development, (15): [PMID:10421630] |
| 6. Chiu, Y L YL, Coronel, E E, Ho, C K CK, Shuman, S S and Rana, T M TM.. (2001) HIV-1 Tat protein interacts with mammalian capping enzyme and stimulates capping of TAR RNA.. The Journal of biological chemistry, (20): [PMID:11278368] |
| 7. Chu, Chun C and 8 more authors.. (2011) Structure of the guanylyltransferase domain of human mRNA capping enzyme.. Proceedings of the National Academy of Sciences of the United States of America, (21): [PMID:21636784] |
