Recombinant (RR672); Rabbit anti Human HDAC1 Antibody; WB, IHC, Flow, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab107012-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab107012-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $177.90 | |
| Ab107012-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $267.90 | |
| Ab107012-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,007.90 |
Recombinant (RR672); Rabbit anti Human HDAC1 Antibody; WB, IHC, Flow, IF, ICC; Unconjugated
| Product Name | Recombinant HDAC1 Antibody - Primary antibody, specific to HDAC1, Rabbit IgG |
|---|---|
| Synonyms | DKFZp686H12203 antibody; GON 10 antibody; HD1 antibody; HDAC 1 antibody; HDAC1 antibody; HDAC1_HUMAN antibody; Histone deacetylase 1 antibody; Reduced potassium dependency yeast homolog like 1 antibody; RPD3 antibody; RPD3L1 antibody; HDAC1; GON-10; HD1; |
| Specifications & Purity | ExactAb™, Validated, Recombinant, High performance, 0.125 mg/mL |
| Host species | Rabbit |
| Specificity | HDAC1 |
| Immunogen | A synthetic peptide corresponding to the C-term of HDAC1 was used as an immunogen. |
| Positive Control | WB: Jurkat, K562, MOLT-4, HeLa, THP-1, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human colon tissue. Flow: HeLa cells. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Recombinant, Validated |
| Product Description | Rabbit anti Human HDAC1 Antibody, Recombinant (RR672), could be used for WB, IHC, Flow, IF, ICC and so on. Application: WB: 1/1000 - 1/2000 IHC: 1/100 - 1/200 Flow: 1/50 - 1/200 IF/ICC: 1/200 - 1/500 Protein Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Post-translational Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1. Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes. Ubiquitinated by CHFR, leading to its degradation by the proteasome. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.125 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant HDAC1 Antibody (Ab107012) - Western Blot
All lanes: Recombinant HDAC1 Antibody (Ab107012) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 61 kDa
Exposure time: 10.0 s
Recombinant HDAC1 Antibody (Ab107012) - Western Blot
All lanes: Recombinant HDAC1 Antibody (Ab107012) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 61 kDa
Exposure time: 19.5 s
Recombinant HDAC1 Antibody (Ab107012) - IF/ICC
IF analysis of HDAC1 (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant HDAC1 Antibody (Ab107012) at 0.25 μg/mL in blocking buffer overnight at 4 °C, and then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant HDAC1 Antibody (Ab107012) - IF/ICC
Recombinant HDAC1 Antibody (Ab107012) staining HDAC1 in Hela cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were
fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant HDAC1 Antibody (Ab107012) (1/500) at 4°C. An Alexa Fluor@ 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant HDAC1 Antibody (Ab107012) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human colon tissue labelling HDAC1 with Recombinant HDAC1 Antibody (Ab107012) at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant HDAC1 Antibody (Ab107012) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant HDAC1 Antibody (Ab107012) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant HDAC1 Antibody (Ab107012) (1/200 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 1/100 - 1/200 |
| WB |
1/1000 - 1/2000
|
| Flow | 1/50 - 1/200 |
| WB |
1/1,000-1/20,000
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1001410 | Certificate of Analysis | Oct 26, 2023 | Ab107012 |
| ZJ23F1001411 | Certificate of Analysis | Oct 26, 2023 | Ab107012 |
