A synthetic peptide derived from human Histone H1.0 (AA 16-30)
Positive Control
WB: U-2 OS and NIH/3T3 cell lysates. IHC: Human Cholangiocarcinoma tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Histone H1.0 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.3 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Histone H1.0 Antibody (Ab107581) - Western Blot All lanes: Recombinant Histone H1.0 Antibody (Ab107581) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 17, 19, 30 kDa Exposure time: 4.9 s
Recombinant Histone H1.0 Antibody (Ab107581) - IHC Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant Histone H1.0 Antibody (Ab107581). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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