Recombinant Histone H1.0 Antibody - Primary antibody, specific to H1-0, Rabbit IgG

    Application:
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Hamster,Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab107581
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab107581-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$89.90
Ab107581-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$269.90
Ab107581-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$379.90
Ab107581-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Recombinant; Rabbit anti Human Histone H1.0 Antibody; WB, IHC; Unconjugated

Basic Description

Product NameRecombinant Histone H1.0 Antibody - Primary antibody, specific to H1-0, Rabbit IgG
SynonymsHistone H1' | Histone H1(0) | H10_HUMAN | H1-0 | Histone H1' | Histone H1(0) | H1F0 | H1FV | H1.0 linker histone | H10 | H1 histone family member 0 antibody | H1(0) antibody | H10 antibody | H10_HUMAN antibody | h1f0 antibody | H1FV antibody | Histone H1'
Specifications & PurityExactAb™, Validated, Recombinant, 0.3 mg/mL
Host speciesRabbit
SpecificityH1-0
ImmunogenA synthetic peptide derived from human Histone H1.0 (AA 16-30)
Positive ControlWB: U-2 OS and NIH/3T3 cell lysates. IHC: Human Cholangiocarcinoma tissue.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Histone H1.0 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.3 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Histone H1.0 Antibody (Ab107581) - Western Blot
All lanes: Recombinant Histone H1.0 Antibody (Ab107581) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 17, 19, 30 kDa
Exposure time: 4.9 s

Recombinant Histone H1.0 Antibody (Ab107581) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant Histone H1.0 Antibody (Ab107581). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

H1F0 Tchem Histone H1.0 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

IHC1/50 - 1/100

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1/50 - 1/100

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0606683Certificate of AnalysisJun 11, 2024 Ab107581
ZJ24F0606684Certificate of AnalysisJun 11, 2024 Ab107581

Related Documents

References

1. Orsolya Leidecker,Juan José Bonfiglio,Thomas Colby,Qi Zhang,Ilian Atanassov,Roko Zaja,Luca Palazzo,Anna Stockum,Ivan Ahel,Ivan Matic.  (2016-10-25)  Serine is a new target residue for endogenous ADP-ribosylation on histones..  Nature chemical biology,  12  ((12)): (998-1000).  [PMID:27723750]
2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
3. Dunham, I I and 24 more authors..  (1999)  The DNA sequence of human chromosome 22..  Nature,    (2):   [PMID:10591208]
4. Collins, John E JE and 12 more authors..  (2004)  A genome annotation-driven approach to cloning the human ORFeome..  Genome biology,      [PMID:15461802]
5. Van Damme, Petra P and 15 more authors..  (2012)  N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB..  Proceedings of the National Academy of Sciences of the United States of America,    (31):   [PMID:22814378]
6. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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