Recombinant; Rabbit anti Human Histone H1.2 Antibody; WB, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab107590-10μl | 10μl | In stock | $89.90 | |
| Ab107590-50μl | 50μl | In stock | $269.90 | |
| Ab107590-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $379.90 | |
| Ab107590-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Recombinant; Rabbit anti Human Histone H1.2 Antibody; WB, IF, ICC; Unconjugated
| Product Name | Recombinant Histone H1.2 Antibody - Primary antibody, specific to H1-2, Rabbit IgG |
|---|---|
| Synonyms | Histone H1c | Histone H1d | Histone H1s-1 | H1 histone family member 2 | H1.a | H12_HUMAN | H1F2 | H1s-1 | HIST1H1C | Histone 1 H1c | Histone cluster 1 H1c | Histone H1.2 | Histone H1c | Histone H1d | Histone H1s-1 | MGC3992 | H1 histone family member 2 a |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.3 mg/mL |
| Host species | Rabbit |
| Specificity | H1-2 |
| Immunogen | A synthetic peptide derived from human Histone H1.2 (AA 125-138) |
| Positive Control | WB: HeLa, MCF7, K562, NIH/3T3 and C6 cell lysates. ICC/IF: K562 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Histone H1.2 Antibody, Recombinant, could be used for WB, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Histone H1.2 Antibody (Ab107590) - Western Blot
All lanes: Recombinant Histone H1.2 Antibody (Ab107590) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 32 kDa
Exposure time: 15.5 s
Recombinant Histone H1.2 Antibody (Ab107590) - Western Blot
All lanes: Recombinant Histone H1.2 Antibody (Ab107590) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 32 kDa
Exposure time: 4.4 s
Recombinant Histone H1.2 Antibody (Ab107590) - IF/ICC
Immunocytochemistry analysis of Histone H1.2 (green) in K562 using Recombinant Histone H1.2 Antibody (Ab107590), and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303783 | Certificate of Analysis | Mar 27, 2024 | Ab107590 |
| ZJ24F0303782 | Certificate of Analysis | Mar 27, 2024 | Ab107590 |
| 1. Orsolya Leidecker,Juan José Bonfiglio,Thomas Colby,Qi Zhang,Ilian Atanassov,Roko Zaja,Luca Palazzo,Anna Stockum,Ivan Ahel,Ivan Matic. (2016-10-25) Serine is a new target residue for endogenous ADP-ribosylation on histones.. Nature chemical biology, 12 ((12)): (998-1000). [PMID:27723750] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Marzluff, William F WF, Gongidi, Preetam P, Woods, Keith R KR, Jin, Jianping J and Maltais, Lois J LJ.. (2002) The human and mouse replication-dependent histone genes.. Genomics, [PMID:12408966] |
| 4. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 5. Goshima, Naoki N and 54 more authors.. (2008) Human protein factory for converting the transcriptome into an in vitro-expressed proteome,.. Nature methods, [PMID:19054851] |
| 6. Weiss, Thomas and 9 more authors.. (2010) Histone H1 variant-specific lysine methylation by G9a/KMT1C and Glp1/KMT1D.. Epigenetics & chromatin, (24): [PMID:20334638] |
| 7. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 8. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 9. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 10. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 11. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
