| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab107594-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $99.90 | |
| Ab107594-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab107594-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $439.90 | |
| Ab107594-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Recombinant; Rabbit anti Human Histone H1.3 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Recombinant Histone H1.3 Antibody - Primary antibody, specific to H1-3, Rabbit IgG |
|---|---|
| Synonyms | Histone H1c | Histone H1s-2 | H1 histone family member 3 | H1.3 | H13_HUMAN | H1F3 | HIST1 H1D | HIST1H1D | Histone 1 H1d | Histone cluster 1 H1d | Histone H1.3 | Histone H1c | MGC138176 | H1 histone family member 3 antibody | H1.3 antibody | H13_HUMAN an |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.2 mg/mL |
| Host species | Rabbit |
| Specificity | H1-3 |
| Immunogen | A synthetic peptide derived from human Histone H1.3 (AA 5-17) |
| Positive Control | WB: HeLa and K562 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Histone H1.3 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Histone H1.3 Antibody (Ab107594) - Western Blot
All lanes: Recombinant Histone H1.3 Antibody (Ab107594) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 32 kDa
Exposure time: 12.4 s
Recombinant Histone H1.3 Antibody (Ab107594) - IF/ICC
Immunocytochemistry analysis of Histone H1.3 (green) in HeLa using Recombinant Histone H1.3 Antibody (Ab107594), and cells were counterstained with DAPI (blue).
Recombinant Histone H1.3 Antibody (Ab107594) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Histone H1.3 Antibody (Ab107594). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0606412 | Certificate of Analysis | Jun 03, 2024 | Ab107594 |
| ZJ24F0606411 | Certificate of Analysis | Jun 03, 2024 | Ab107594 |
| 1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 2. Albig, W W, Kardalinou, E E, Drabent, B B, Zimmer, A A and Doenecke, D D.. (1991) Isolation and characterization of two human H1 histone genes within clusters of core histone genes.. Genomics, [PMID:1916825] |
| 3. Marzluff, William F WF, Gongidi, Preetam P, Woods, Keith R KR, Jin, Jianping J and Maltais, Lois J LJ.. (2002) The human and mouse replication-dependent histone genes.. Genomics, [PMID:12408966] |
| 4. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 5. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 6. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 7. Xie, Zhongyu and 26 more authors.. (2016) Metabolic Regulation of Gene Expression by Histone Lysine β-Hydroxybutyrylation.. Molecular cell, (21): [PMID:27105115] |
| 8. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
