Recombinant; Rabbit anti Human HJURP Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab108136-10μl | 10μl | In stock | $69.90 | |
| Ab108136-50μl | 50μl | In stock | $199.90 | |
| Ab108136-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab108136-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human HJURP Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant HJURP Antibody - Primary antibody, specific to HJURP, Rabbit IgG | |
|---|---|---|
| Synonyms | 14-3-3-associated AKT substrate antibody | FAKTS antibody | fetal liver expressing gene 1 antibody | Fetal liver-expressing gene 1 protein antibody | FLEG1 antibody | hFLEG1 antibody | HJURP antibody | Holliday junction recognition protein antibody | Up-r | |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.7 mg/mL | |
| Host species | Rabbit | |
| Specificity | HJURP | |
| Immunogen | Recombinant protein of human HJURP (AA 11-165) | |
| Positive Control | WB: MDA-MB-231, HeLa, A549 and MCF7 cell lysates. IHC: Human lung cancer tissue. | |
| Conjugation | Unconjugated | |
| Grade | ExactAb™, Recombinant, Validated | |
| Product Description | Rabbit anti Human HJURP Antibody, Recombinant, could be used for WB, IHC and so on. Application:
Relevance Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.7 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant HJURP Antibody (Ab108136) - Western Blot
All lanes: Recombinant HJURP Antibody (Ab108136) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 84 kDa
Observed band size: 90 kDa
Exposure time: 11.4 s
Recombinant HJURP Antibody (Ab108136) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant HJURP Antibody (Ab108136). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303880 | Certificate of Analysis | Apr 02, 2024 | Ab108136 |
| ZJ24F0404052 | Certificate of Analysis | Apr 02, 2024 | Ab108136 |
| 1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 2. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 3. Foltz, Daniel R DR and 5 more authors.. (2006) The human CENP-A centromeric nucleosome-associated complex.. Nature cell biology, [PMID:16622419] |
| 4. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243] |
| 5. Kato, Tatsuya T and 8 more authors.. (2007) Activation of Holliday junction recognizing protein involved in the chromosomal stability and immortality of cancer cells.. Cancer research, (15): [PMID:17823411] |
| 6. Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31): [PMID:17974005] |
| 7. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 8. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 9. Foltz, Daniel R DR and 7 more authors.. (2009) Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP.. Cell, (1): [PMID:19410544] |
| 10. Dunleavy, Elaine M EM and 8 more authors.. (2009) HJURP is a cell-cycle-dependent maintenance and deposition factor of CENP-A at centromeres.. Cell, (1): [PMID:19410545] |
| 11. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 12. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 13. Hu, Hao H and 11 more authors.. (2011) Structure of a CENP-A-histone H4 heterodimer in complex with chaperone HJURP.. Genes & development, (1): [PMID:21478274] |
| 14. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 15. Roulland, Yohan Y and 19 more authors.. (2016) The Flexible Ends of CENP-A Nucleosome Are Required for Mitotic Fidelity.. Molecular cell, (18): [PMID:27499292] |
