Basic Description

Product Name

Recombinant HNF-4-alpha Antibody - Primary antibody, specific to HNF4A, Rabbit IgG

Synonyms

Specifications & Purity

ExactAb™, Validated, Recombinant, 0.7 mg/mL

Host species

Rabbit

Specificity

HNF4A

Immunogen

Recombinant protein of human HNF-4-alpha (AA 224-474)

Positive Control

WB: A549 and Hep G2 cell lysates.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Product Description

Rabbit anti Human HNF-4-alpha Antibody, Recombinant, could be used for WB and so on.

Application:

WB: 1/500-1/1000

Protein Function

Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.

Post-translational

Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	0.7 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant HNF-4-alpha Antibody (Ab108377) - Western Blot
All lanes: Recombinant HNF-4-alpha Antibody (Ab108377) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 53 kDa
Observed band size: 51 kDa
Exposure time: 10.0 s

Recombinant HNF-4-alpha Antibody (Ab108377) - Western Blot
All lanes: Recombinant HNF-4-alpha Antibody (Ab108377) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 53 kDa
Observed band size: 51 kDa
Exposure time: 4.5 s

Associated Targets(Human)

HNF4A Tchem Hepatocyte nuclear factor 4-alpha (0 Activities)

Discover Target: HNF4A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Application

Application	Dilution info
WB	1/500-1/1000 "> 1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0303359	Certificate of Analysis	Mar 19, 2024	Ab108377
ZJ24F0303358	Certificate of Analysis	Mar 19, 2024	Ab108377

FAQs and Articles

FAQ

Protocol of Western Blotting For High Molecular Weights

Categories: Protocols

Tags:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

References

1. Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J. (2001) The DNA sequence and comparative analysis of human chromosome 20.. Nature, 414 (6866): (865-71). [PMID:11780052] [10.1021/op500134e]
2. Chartier FL, Bossu JP, Laudet V, Fruchart JC, Laine B. (1994) Cloning and sequencing of cDNAs encoding the human hepatocyte nuclear factor 4 indicate the presence of two isoforms in human liver.. Gene, 147 (2): (269-72). [PMID:7926813] [10.1021/op500134e]
3. Kritis AA, Argyrokastritis A, Moschonas NK, Power S, Katrakili N, Zannis VI, Cereghini S, Talianidis I. (1996) Isolation and characterization of a third isoform of human hepatocyte nuclear factor 4.. Gene, 173 (2): (275-80). [PMID:8964514] [10.1021/op500134e]
4. Drewes T, Senkel S, Holewa B, Ryffel GU. (1996) Human hepatocyte nuclear factor 4 isoforms are encoded by distinct and differentially expressed genes.. Mol Cell Biol, 16 (3): (925-31). [PMID:8622695] [10.1021/op500134e]
5. Hani EH, Suaud L, Boutin P, Chèvre JC, Durand E, Philippi A, Demenais F, Vionnet N, Furuta H, Velho G, Bell GI, Laine B, Froguel P. (1998) A missense mutation in hepatocyte nuclear factor-4 alpha, resulting in a reduced transactivation activity, in human late-onset non-insulin-dependent diabetes mellitus.. J Clin Invest, 101 (3): (521-6). [PMID:9449683] [10.1021/op500134e]
6. Yuan X, Ta TC, Lin M, Evans JR, Dong Y, Bolotin E, Sherman MA, Forman BM, Sladek FM. (2009) Identification of an endogenous ligand bound to a native orphan nuclear receptor.. PLoS ONE, 4 (5): (e5609). [PMID:19440305] [10.1021/op500134e]
7. Kiselyuk A, Lee SH, Farber-Katz S, Zhang M, Athavankar S, Cohen T, Pinkerton AB, Ye M, Bushway P, Richardson AD et al.. (2012) HNF4α antagonists discovered by a high-throughput screen for modulators of the human insulin promoter.. Chem Biol, 19 (7): (806-18). [PMID:22840769] [10.1021/op500134e]
8. Yamagata K, Furuta H, Oda N, Kaisaki PJ, Menzel S, Cox NJ, Fajans SS, Signorini S, Stoffel M, Bell GI. (1996) Mutations in the hepatocyte nuclear factor-4alpha gene in maturity-onset diabetes of the young (MODY1).. Nature, 384 (6608): (458-60). [PMID:8945471] [10.1021/op500134e]
9. Furuta H, Iwasaki N, Oda N, Hinokio Y, Horikawa Y, Yamagata K, Yano N, Sugahiro J, Ogata M, Ohgawara H et al.. (1997) Organization and partial sequence of the hepatocyte nuclear factor-4 alpha/MODY1 gene and identification of a missense mutation, R127W, in a Japanese family with MODY.. Diabetes, 46 (10): (1652-7). [PMID:9313765] [10.1021/op500134e]
10. Solt LA, Wang Y, Banerjee S, Hughes T, Kojetin DJ, Lundasen T, Shin Y, Liu J, Cameron MD, Noel R et al.. (2012) Regulation of circadian behaviour and metabolism by synthetic REV-ERB agonists.. Nature, 485 (7396): (62-8). [PMID:22460951] [10.1021/op500134e]
11. Montanari R, Saccoccia F, Scotti E, Crestani M, Godio C, Gilardi F, Loiodice F, Fracchiolla G, Laghezza A, Tortorella P, Lavecchia A, Novellino E, Mazza F, Aschi M, Pochetti G.. (2008) Crystal structure of the peroxisome proliferator-activated receptor gamma (PPARgamma) ligand binding domain complexed with a novel partial agonist: a new region of the hydrophobic pocket could be exploited for drug design.. J Med Chem, 51 (24): (7768-7776). [PMID:19053776] [10.1021/jm800733h]
12. Le Guével R, Oger F, Lecorgne A, Dudasova Z, Chevance S, Bondon A, Barath P, Simonneaux G, Salbert G.. (2009) Identification of small molecule regulators of the nuclear receptor HNF4alpha based on naphthofuran scaffolds.. Bioorg Med Chem, 17 (19): (7021-7030). [PMID:19729315] [10.1016/j.bmc.2009.07.079]
13. Ktistaki, E E, Ktistakis, N T NT, Papadogeorgaki, E E and Talianidis, I I.. (1995) Recruitment of hepatocyte nuclear factor 4 into specific intranuclear compartments depends on tyrosine phosphorylation that affects its DNA-binding and transactivation potential.. Proceedings of the National Academy of Sciences of the United States of America, (10): [PMID:7568236]
14. Bulman, M P MP and 6 more authors.. (1997) A missense mutation in the hepatocyte nuclear factor 4 alpha gene in a UK pedigree with maturity-onset diabetes of the young.. Diabetologia, [PMID:9243109]
15. Møller, A M AM and 8 more authors.. (1997) Studies of the genetic variability of the coding region of the hepatocyte nuclear factor-4alpha in Caucasians with maturity onset NIDDM.. Diabetologia, [PMID:9267996]
16. Navas, M A MA, Munoz-Elias, E J EJ, Kim, J J, Shih, D D and Stoffel, M M.. (1999) Functional characterization of the MODY1 gene mutations HNF4(R127W), HNF4(V255M), and HNF4(E276Q).. Diabetes, [PMID:10389854]
17. Hong, Yu Holly YH, Varanasi, Usha S US, Yang, Wenbo W and Leff, Todd T.. (2003) AMP-activated protein kinase regulates HNF4alpha transcriptional activity by inhibiting dimer formation and decreasing protein stability.. The Journal of biological chemistry, (25): [PMID:12740371]
18. Pearson, Ewan R ER and 8 more authors.. (2007) Macrosomia and hyperinsulinaemic hypoglycaemia in patients with heterozygous mutations in the HNF4A gene.. PLoS medicine, [PMID:17407387]
19. Yokoyama, Atsushi A and 6 more authors.. (2011) Multiple post-translational modifications in hepatocyte nuclear factor 4α.. Biochemical and biophysical research communications, (15): [PMID:21708125]
20. Stanescu, Diana E DE, Hughes, Nkecha N, Kaplan, Bernard B, Stanley, Charles A CA and De León, Diva D DD.. (2012) Novel presentations of congenital hyperinsulinism due to mutations in the MODY genes: HNF1A and HNF4A.. The Journal of clinical endocrinology and metabolism, [PMID:22802087]
21. Hamilton, Alexander J AJ and 13 more authors.. (2014) The HNF4A R76W mutation causes atypical dominant Fanconi syndrome in addition to a β cell phenotype.. Journal of medical genetics, [PMID:24285859]
22. Tsai, Tsung-Yuan TY and 6 more authors.. (2017) RNA helicase DDX3 maintains lipid homeostasis through upregulation of the microsomal triglyceride transfer protein by interacting with HNF4 and SHP.. Scientific reports, (27): [PMID:28128295]
23. Qu, Meng M, Duffy, Tomas T, Hirota, Tsuyoshi T and Kay, Steve A SA.. (2018) Nuclear receptor HNF4A transrepresses CLOCK:BMAL1 and modulates tissue-specific circadian networks.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:30530698]
24. DeLaForest, Ann A and 8 more authors.. (2018) HNF4A Regulates the Formation of Hepatic Progenitor Cells from Human iPSC-Derived Endoderm by Facilitating Efficient Recruitment of RNA Pol II.. Genes, (28): [PMID:30597922]

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

SKU	Size	Availability	Price
Ab108377-10μl	10μl	In stock	$66.90
Ab108377-50μl	50μl	In stock	$199.90
Ab108377-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab108377-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,599.90