Recombinant; Rabbit anti Human HNF-4-alpha Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab108377-10μl | 10μl | In stock | $66.90 | |
| Ab108377-50μl | 50μl | In stock | $199.90 | |
| Ab108377-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab108377-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human HNF-4-alpha Antibody; WB; Unconjugated
| Product Name | Recombinant HNF-4-alpha Antibody - Primary antibody, specific to HNF4A, Rabbit IgG | |
|---|---|---|
| Synonyms | FLJ39654 antibody | FRTS4 antibody | Hepatic nuclear factor 4 alpha antibody | Hepatocyte nuclear factor 4 alpha antibody | Hepatocyte nuclear factor 4 antibody | Hepatocyte nuclear factor 4-alpha antibody | HNF 4 alpha antibody | HNF 4 antibody | HNF-4-a | |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.7 mg/mL | |
| Host species | Rabbit | |
| Specificity | HNF4A | |
| Immunogen | Recombinant protein of human HNF-4-alpha (AA 224-474) | |
| Positive Control | WB: A549 and Hep G2 cell lysates. | |
| Conjugation | Unconjugated | |
| Grade | ExactAb™, Recombinant, Validated | |
| Product Description | Rabbit anti Human HNF-4-alpha Antibody, Recombinant, could be used for WB and so on. Application:
Protein Function Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. Post-translational Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.7 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant HNF-4-alpha Antibody (Ab108377) - Western Blot
All lanes: Recombinant HNF-4-alpha Antibody (Ab108377) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 51 kDa
Exposure time: 10.0 s
Recombinant HNF-4-alpha Antibody (Ab108377) - Western Blot
All lanes: Recombinant HNF-4-alpha Antibody (Ab108377) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 51 kDa
Exposure time: 4.5 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303359 | Certificate of Analysis | Mar 19, 2024 | Ab108377 |
| ZJ24F0303358 | Certificate of Analysis | Mar 19, 2024 | Ab108377 |
| 1. Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J. (2001) The DNA sequence and comparative analysis of human chromosome 20.. Nature, 414 (6866): (865-71). [PMID:11780052] |
| 2. Chartier FL, Bossu JP, Laudet V, Fruchart JC, Laine B. (1994) Cloning and sequencing of cDNAs encoding the human hepatocyte nuclear factor 4 indicate the presence of two isoforms in human liver.. Gene, 147 (2): (269-72). [PMID:7926813] |
| 3. Kritis AA, Argyrokastritis A, Moschonas NK, Power S, Katrakili N, Zannis VI, Cereghini S, Talianidis I. (1996) Isolation and characterization of a third isoform of human hepatocyte nuclear factor 4.. Gene, 173 (2): (275-80). [PMID:8964514] |
| 4. Drewes T, Senkel S, Holewa B, Ryffel GU. (1996) Human hepatocyte nuclear factor 4 isoforms are encoded by distinct and differentially expressed genes.. Mol Cell Biol, 16 (3): (925-31). [PMID:8622695] |
| 5. Hani EH, Suaud L, Boutin P, Chèvre JC, Durand E, Philippi A, Demenais F, Vionnet N, Furuta H, Velho G, Bell GI, Laine B, Froguel P. (1998) A missense mutation in hepatocyte nuclear factor-4 alpha, resulting in a reduced transactivation activity, in human late-onset non-insulin-dependent diabetes mellitus.. J Clin Invest, 101 (3): (521-6). [PMID:9449683] |
| 6. Yuan X, Ta TC, Lin M, Evans JR, Dong Y, Bolotin E, Sherman MA, Forman BM, Sladek FM. (2009) Identification of an endogenous ligand bound to a native orphan nuclear receptor.. PLoS ONE, 4 (5): (e5609). [PMID:19440305] |
| 7. Kiselyuk A, Lee SH, Farber-Katz S, Zhang M, Athavankar S, Cohen T, Pinkerton AB, Ye M, Bushway P, Richardson AD et al.. (2012) HNF4α antagonists discovered by a high-throughput screen for modulators of the human insulin promoter.. Chem Biol, 19 (7): (806-18). [PMID:22840769] |
| 8. Yamagata K, Furuta H, Oda N, Kaisaki PJ, Menzel S, Cox NJ, Fajans SS, Signorini S, Stoffel M, Bell GI. (1996) Mutations in the hepatocyte nuclear factor-4alpha gene in maturity-onset diabetes of the young (MODY1).. Nature, 384 (6608): (458-60). [PMID:8945471] |
| 9. Furuta H, Iwasaki N, Oda N, Hinokio Y, Horikawa Y, Yamagata K, Yano N, Sugahiro J, Ogata M, Ohgawara H et al.. (1997) Organization and partial sequence of the hepatocyte nuclear factor-4 alpha/MODY1 gene and identification of a missense mutation, R127W, in a Japanese family with MODY.. Diabetes, 46 (10): (1652-7). [PMID:9313765] |
| 10. Solt LA, Wang Y, Banerjee S, Hughes T, Kojetin DJ, Lundasen T, Shin Y, Liu J, Cameron MD, Noel R et al.. (2012) Regulation of circadian behaviour and metabolism by synthetic REV-ERB agonists.. Nature, 485 (7396): (62-8). [PMID:22460951] |
| 11. Montanari R, Saccoccia F, Scotti E, Crestani M, Godio C, Gilardi F, Loiodice F, Fracchiolla G, Laghezza A, Tortorella P, Lavecchia A, Novellino E, Mazza F, Aschi M, Pochetti G.. (2008) Crystal structure of the peroxisome proliferator-activated receptor gamma (PPARgamma) ligand binding domain complexed with a novel partial agonist: a new region of the hydrophobic pocket could be exploited for drug design.. J Med Chem, 51 (24): (7768-7776). [PMID:19053776] |
| 12. Le Guével R, Oger F, Lecorgne A, Dudasova Z, Chevance S, Bondon A, Barath P, Simonneaux G, Salbert G.. (2009) Identification of small molecule regulators of the nuclear receptor HNF4alpha based on naphthofuran scaffolds.. Bioorg Med Chem, 17 (19): (7021-7030). [PMID:19729315] |
| 13. Ktistaki, E E, Ktistakis, N T NT, Papadogeorgaki, E E and Talianidis, I I.. (1995) Recruitment of hepatocyte nuclear factor 4 into specific intranuclear compartments depends on tyrosine phosphorylation that affects its DNA-binding and transactivation potential.. Proceedings of the National Academy of Sciences of the United States of America, (10): [PMID:7568236] |
| 14. Bulman, M P MP and 6 more authors.. (1997) A missense mutation in the hepatocyte nuclear factor 4 alpha gene in a UK pedigree with maturity-onset diabetes of the young.. Diabetologia, [PMID:9243109] |
| 15. Møller, A M AM and 8 more authors.. (1997) Studies of the genetic variability of the coding region of the hepatocyte nuclear factor-4alpha in Caucasians with maturity onset NIDDM.. Diabetologia, [PMID:9267996] |
| 16. Navas, M A MA, Munoz-Elias, E J EJ, Kim, J J, Shih, D D and Stoffel, M M.. (1999) Functional characterization of the MODY1 gene mutations HNF4(R127W), HNF4(V255M), and HNF4(E276Q).. Diabetes, [PMID:10389854] |
| 17. Hong, Yu Holly YH, Varanasi, Usha S US, Yang, Wenbo W and Leff, Todd T.. (2003) AMP-activated protein kinase regulates HNF4alpha transcriptional activity by inhibiting dimer formation and decreasing protein stability.. The Journal of biological chemistry, (25): [PMID:12740371] |
| 18. Pearson, Ewan R ER and 8 more authors.. (2007) Macrosomia and hyperinsulinaemic hypoglycaemia in patients with heterozygous mutations in the HNF4A gene.. PLoS medicine, [PMID:17407387] |
| 19. Yokoyama, Atsushi A and 6 more authors.. (2011) Multiple post-translational modifications in hepatocyte nuclear factor 4α.. Biochemical and biophysical research communications, (15): [PMID:21708125] |
| 20. Stanescu, Diana E DE, Hughes, Nkecha N, Kaplan, Bernard B, Stanley, Charles A CA and De León, Diva D DD.. (2012) Novel presentations of congenital hyperinsulinism due to mutations in the MODY genes: HNF1A and HNF4A.. The Journal of clinical endocrinology and metabolism, [PMID:22802087] |
| 21. Hamilton, Alexander J AJ and 13 more authors.. (2014) The HNF4A R76W mutation causes atypical dominant Fanconi syndrome in addition to a β cell phenotype.. Journal of medical genetics, [PMID:24285859] |
| 22. Tsai, Tsung-Yuan TY and 6 more authors.. (2017) RNA helicase DDX3 maintains lipid homeostasis through upregulation of the microsomal triglyceride transfer protein by interacting with HNF4 and SHP.. Scientific reports, (27): [PMID:28128295] |
| 23. Qu, Meng M, Duffy, Tomas T, Hirota, Tsuyoshi T and Kay, Steve A SA.. (2018) Nuclear receptor HNF4A transrepresses CLOCK:BMAL1 and modulates tissue-specific circadian networks.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:30530698] |
| 24. DeLaForest, Ann A and 8 more authors.. (2018) HNF4A Regulates the Formation of Hepatic Progenitor Cells from Human iPSC-Derived Endoderm by Facilitating Efficient Recruitment of RNA Pol II.. Genes, (28): [PMID:30597922] |
